Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS12410 and ABZR87_RS12415 are separated by 142 nucleotides ABZR87_RS12415 and ABZR87_RS12420 overlap by 4 nucleotides ABZR87_RS12420 and ABZR87_RS12425 are separated by 5 nucleotides
ABZR87_RS12410: ABZR87_RS12410 - LysR substrate-binding domain-containing protein, at 2,630,624 to 2,631,553
_RS12410
ABZR87_RS12415: ABZR87_RS12415 - NAD(P)H-dependent oxidoreductase, at 2,631,696 to 2,632,379
_RS12415
ABZR87_RS12420: ABZR87_RS12420 - phosphodiesterase, at 2,632,376 to 2,633,224
_RS12420
ABZR87_RS12425: ABZR87_RS12425 - ABC transporter ATP-binding protein, at 2,633,230 to 2,634,291
_RS12425
Position (kb)
2631
2632
2633 Strain fitness (log2 ratio)
-2
-1
0
1 at 2630.716 kb on + strand at 2630.716 kb on + strand at 2630.716 kb on + strand at 2630.717 kb on - strand, within ABZR87_RS12410 at 2630.717 kb on - strand, within ABZR87_RS12410 at 2630.717 kb on - strand, within ABZR87_RS12410 at 2630.742 kb on + strand, within ABZR87_RS12410 at 2630.742 kb on + strand, within ABZR87_RS12410 at 2630.743 kb on - strand, within ABZR87_RS12410 at 2631.048 kb on + strand, within ABZR87_RS12410 at 2631.048 kb on + strand, within ABZR87_RS12410 at 2631.049 kb on - strand, within ABZR87_RS12410 at 2631.661 kb on - strand at 2631.710 kb on - strand at 2631.710 kb on - strand at 2631.710 kb on - strand at 2632.005 kb on + strand, within ABZR87_RS12415 at 2632.006 kb on - strand, within ABZR87_RS12415 at 2632.006 kb on - strand, within ABZR87_RS12415 at 2632.038 kb on + strand, within ABZR87_RS12415 at 2632.038 kb on + strand, within ABZR87_RS12415 at 2632.039 kb on - strand, within ABZR87_RS12415 at 2632.039 kb on - strand, within ABZR87_RS12415 at 2632.039 kb on - strand, within ABZR87_RS12415 at 2632.377 kb on + strand at 2632.378 kb on - strand at 2632.437 kb on + strand at 2632.437 kb on + strand at 2632.438 kb on - strand at 2632.488 kb on + strand, within ABZR87_RS12420 at 2632.888 kb on - strand, within ABZR87_RS12420 at 2633.046 kb on + strand, within ABZR87_RS12420 at 2633.046 kb on + strand, within ABZR87_RS12420 at 2633.122 kb on + strand, within ABZR87_RS12420 at 2633.123 kb on - strand, within ABZR87_RS12420 at 2633.146 kb on - strand at 2633.146 kb on - strand at 2633.194 kb on + strand at 2633.194 kb on + strand at 2633.195 kb on - strand at 2633.195 kb on - strand at 2633.318 kb on - strand at 2633.319 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 2,630,716 + +1.1 2,630,716 + -0.3 2,630,716 + -1.2 2,630,717 - ABZR87_RS12410 0.10 -1.5 2,630,717 - ABZR87_RS12410 0.10 +0.8 2,630,717 - ABZR87_RS12410 0.10 +0.2 2,630,742 + ABZR87_RS12410 0.13 +0.5 2,630,742 + ABZR87_RS12410 0.13 +0.2 2,630,743 - ABZR87_RS12410 0.13 +0.5 2,631,048 + ABZR87_RS12410 0.46 -0.9 2,631,048 + ABZR87_RS12410 0.46 -2.0 2,631,049 - ABZR87_RS12410 0.46 +0.3 2,631,661 - -0.2 2,631,710 - +0.8 2,631,710 - +0.5 2,631,710 - -0.5 2,632,005 + ABZR87_RS12415 0.45 -1.3 2,632,006 - ABZR87_RS12415 0.45 -0.3 2,632,006 - ABZR87_RS12415 0.45 -0.1 2,632,038 + ABZR87_RS12415 0.50 +0.2 2,632,038 + ABZR87_RS12415 0.50 -0.5 2,632,039 - ABZR87_RS12415 0.50 -1.0 2,632,039 - ABZR87_RS12415 0.50 +0.3 2,632,039 - ABZR87_RS12415 0.50 +1.2 2,632,377 + -0.7 2,632,378 - -0.2 2,632,437 + +0.2 2,632,437 + -0.7 2,632,438 - -0.9 2,632,488 + ABZR87_RS12420 0.13 +0.5 2,632,888 - ABZR87_RS12420 0.60 +0.3 2,633,046 + ABZR87_RS12420 0.79 +0.2 2,633,046 + ABZR87_RS12420 0.79 +0.6 2,633,122 + ABZR87_RS12420 0.88 -1.4 2,633,123 - ABZR87_RS12420 0.88 -0.6 2,633,146 - -1.5 2,633,146 - -0.1 2,633,194 + +0.1 2,633,194 + +0.8 2,633,195 - -0.4 2,633,195 - -2.0 2,633,318 - -1.2 2,633,319 + +0.9
Or see this region's nucleotide sequence