Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10665 and ABZR87_RS10670 are separated by 149 nucleotides ABZR87_RS10670 and ABZR87_RS10675 are separated by 192 nucleotides ABZR87_RS10675 and ABZR87_RS10680 are separated by 308 nucleotides
ABZR87_RS10665: ABZR87_RS10665 - aspartate kinase, at 2,256,965 to 2,258,215
_RS10665
ABZR87_RS10670: ABZR87_RS10670 - tRNA-Ser, at 2,258,365 to 2,258,458
_RS10670
ABZR87_RS10675: ABZR87_RS10675 - enoyl-ACP reductase FabI, at 2,258,651 to 2,259,445
_RS10675
ABZR87_RS10680: ABZR87_RS10680 - extracellular solute-binding protein, at 2,259,754 to 2,261,640
_RS10680
Position (kb)
2258
2259
2260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2257.711 kb on + strand, within ABZR87_RS10665 at 2258.213 kb on + strand at 2258.213 kb on + strand at 2258.214 kb on - strand at 2258.214 kb on - strand at 2258.214 kb on - strand at 2258.487 kb on + strand at 2258.487 kb on + strand at 2258.488 kb on - strand at 2258.595 kb on - strand at 2258.647 kb on + strand at 2258.647 kb on + strand at 2258.647 kb on + strand at 2258.647 kb on + strand at 2258.647 kb on + strand at 2259.457 kb on - strand at 2259.484 kb on - strand at 2259.484 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.547 kb on - strand at 2259.562 kb on + strand at 2259.563 kb on - strand at 2259.676 kb on + strand at 2259.677 kb on - strand at 2259.711 kb on + strand at 2259.857 kb on - strand at 2259.872 kb on - strand at 2259.872 kb on - strand at 2259.898 kb on + strand at 2260.044 kb on + strand, within ABZR87_RS10680 at 2260.044 kb on + strand, within ABZR87_RS10680 at 2260.045 kb on - strand, within ABZR87_RS10680 at 2260.189 kb on + strand, within ABZR87_RS10680 at 2260.189 kb on + strand, within ABZR87_RS10680 at 2260.189 kb on + strand, within ABZR87_RS10680 at 2260.190 kb on - strand, within ABZR87_RS10680 at 2260.190 kb on - strand, within ABZR87_RS10680 at 2260.196 kb on - strand, within ABZR87_RS10680 at 2260.196 kb on - strand, within ABZR87_RS10680 at 2260.231 kb on + strand, within ABZR87_RS10680
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 2,257,711 + ABZR87_RS10665 0.60 -2.5 2,258,213 + +0.4 2,258,213 + -0.9 2,258,214 - +0.3 2,258,214 - -0.4 2,258,214 - +0.2 2,258,487 + +0.2 2,258,487 + -1.0 2,258,488 - -0.9 2,258,595 - +0.0 2,258,647 + +0.3 2,258,647 + -1.4 2,258,647 + -0.3 2,258,647 + +0.2 2,258,647 + -0.7 2,259,457 - +2.5 2,259,484 - +1.7 2,259,484 - +0.8 2,259,547 - -0.1 2,259,547 - -0.7 2,259,547 - -0.6 2,259,547 - -0.4 2,259,547 - -0.3 2,259,547 - -0.2 2,259,547 - +0.2 2,259,562 + +0.4 2,259,563 - +0.2 2,259,676 + -0.1 2,259,677 - +2.1 2,259,711 + +0.5 2,259,857 - -0.6 2,259,872 - -0.9 2,259,872 - -3.4 2,259,898 + -0.0 2,260,044 + ABZR87_RS10680 0.15 -0.8 2,260,044 + ABZR87_RS10680 0.15 -0.1 2,260,045 - ABZR87_RS10680 0.15 +0.7 2,260,189 + ABZR87_RS10680 0.23 -0.2 2,260,189 + ABZR87_RS10680 0.23 -0.2 2,260,189 + ABZR87_RS10680 0.23 -0.3 2,260,190 - ABZR87_RS10680 0.23 -2.5 2,260,190 - ABZR87_RS10680 0.23 -0.7 2,260,196 - ABZR87_RS10680 0.23 -0.7 2,260,196 - ABZR87_RS10680 0.23 -2.5 2,260,231 + ABZR87_RS10680 0.25 -1.0
Or see this region's nucleotide sequence