Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01570 and ABZR87_RS01575 overlap by 4 nucleotides ABZR87_RS01575 and ABZR87_RS01580 are separated by 32 nucleotides ABZR87_RS01580 and ABZR87_RS01585 are separated by 1 nucleotides ABZR87_RS01585 and ABZR87_RS01590 are separated by 39 nucleotides
ABZR87_RS01570: ABZR87_RS01570 - thiamine-phosphate kinase, at 331,793 to 332,878
_RS01570
ABZR87_RS01575: ABZR87_RS01575 - phosphatidylglycerophosphatase A, at 332,875 to 333,453
_RS01575
ABZR87_RS01580: ABZR87_RS01580 - CinA family protein, at 333,486 to 333,986
_RS01580
ABZR87_RS01585: ABZR87_RS01585 - hypothetical protein, at 333,988 to 334,206
_RS01585
ABZR87_RS01590: ABZR87_RS01590 - orotidine-5'-phosphate decarboxylase, at 334,246 to 335,061
_RS01590
Position (kb) 333
334 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 333.480 kb on - strand at 333.480 kb on - strand at 333.613 kb on + strand, within ABZR87_RS01580 at 333.614 kb on - strand, within ABZR87_RS01580 at 333.614 kb on - strand, within ABZR87_RS01580 at 333.794 kb on + strand, within ABZR87_RS01580 at 333.989 kb on + strand at 333.989 kb on + strand at 333.990 kb on - strand at 333.990 kb on - strand at 334.534 kb on + strand, within ABZR87_RS01590 at 334.534 kb on + strand, within ABZR87_RS01590 at 334.535 kb on - strand, within ABZR87_RS01590
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 333,480 - +1.4 333,480 - -0.2 333,613 + ABZR87_RS01580 0.25 -2.2 333,614 - ABZR87_RS01580 0.26 -0.4 333,614 - ABZR87_RS01580 0.26 -0.3 333,794 + ABZR87_RS01580 0.61 -0.0 333,989 + +1.9 333,989 + -0.1 333,990 - +3.1 333,990 - -1.5 334,534 + ABZR87_RS01590 0.35 +0.5 334,534 + ABZR87_RS01590 0.35 -0.5 334,535 - ABZR87_RS01590 0.35 +2.3
Or see this region's nucleotide sequence