Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS01080

Experiment: root EcoFAB sample 1; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS01075 and ABZR87_RS01080 are separated by 224 nucleotidesABZR87_RS01080 and ABZR87_RS01085 are separated by 94 nucleotidesABZR87_RS01085 and ABZR87_RS01090 overlap by 4 nucleotides ABZR87_RS01075: ABZR87_RS01075 - MprA protease, GlyGly-CTERM protein-sorting domain-containing form, at 215,473 to 217,122 _RS01075 ABZR87_RS01080: ABZR87_RS01080 - S8 family serine peptidase, at 217,347 to 219,401 _RS01080 ABZR87_RS01085: ABZR87_RS01085 - rhombosortase, at 219,496 to 220,116 _RS01085 ABZR87_RS01090: ABZR87_RS01090 - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 220,113 to 222,956 _RS01090 Position (kb) 217 218 219 220Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 216.584 kb on - strand, within ABZR87_RS01075at 216.747 kb on + strand, within ABZR87_RS01075at 216.748 kb on - strand, within ABZR87_RS01075at 216.937 kb on + strand, within ABZR87_RS01075at 216.937 kb on + strand, within ABZR87_RS01075at 216.937 kb on + strand, within ABZR87_RS01075at 216.937 kb on + strand, within ABZR87_RS01075at 216.938 kb on - strand, within ABZR87_RS01075at 216.938 kb on - strand, within ABZR87_RS01075at 216.938 kb on - strand, within ABZR87_RS01075at 217.014 kb on + strandat 217.088 kb on + strandat 217.131 kb on + strandat 217.131 kb on + strandat 217.131 kb on + strandat 217.132 kb on - strandat 217.132 kb on - strandat 217.132 kb on - strandat 217.132 kb on - strandat 217.221 kb on + strandat 217.222 kb on - strandat 217.222 kb on - strandat 217.243 kb on + strandat 217.244 kb on - strandat 217.244 kb on - strandat 217.252 kb on + strandat 217.252 kb on + strandat 217.253 kb on - strandat 217.256 kb on + strandat 217.407 kb on + strandat 217.407 kb on + strandat 217.407 kb on + strandat 217.408 kb on - strandat 217.408 kb on - strandat 217.408 kb on - strandat 217.487 kb on + strandat 217.487 kb on + strandat 217.602 kb on + strand, within ABZR87_RS01080at 217.602 kb on + strand, within ABZR87_RS01080at 217.602 kb on + strand, within ABZR87_RS01080at 217.602 kb on + strand, within ABZR87_RS01080at 217.602 kb on + strand, within ABZR87_RS01080at 217.603 kb on - strand, within ABZR87_RS01080at 217.603 kb on - strand, within ABZR87_RS01080at 217.646 kb on + strand, within ABZR87_RS01080at 217.713 kb on + strand, within ABZR87_RS01080at 217.713 kb on + strand, within ABZR87_RS01080at 217.714 kb on - strand, within ABZR87_RS01080at 217.714 kb on - strand, within ABZR87_RS01080at 217.714 kb on - strand, within ABZR87_RS01080at 217.714 kb on - strand, within ABZR87_RS01080at 217.844 kb on + strand, within ABZR87_RS01080at 217.845 kb on - strand, within ABZR87_RS01080at 217.845 kb on - strand, within ABZR87_RS01080at 217.862 kb on + strand, within ABZR87_RS01080at 217.862 kb on + strand, within ABZR87_RS01080at 217.862 kb on + strand, within ABZR87_RS01080at 217.947 kb on - strand, within ABZR87_RS01080at 217.984 kb on - strand, within ABZR87_RS01080at 218.075 kb on + strand, within ABZR87_RS01080at 218.075 kb on + strand, within ABZR87_RS01080at 218.076 kb on - strand, within ABZR87_RS01080at 218.076 kb on - strand, within ABZR87_RS01080at 218.076 kb on - strand, within ABZR87_RS01080at 218.125 kb on - strand, within ABZR87_RS01080at 218.133 kb on + strand, within ABZR87_RS01080at 218.134 kb on - strand, within ABZR87_RS01080at 218.134 kb on - strand, within ABZR87_RS01080at 218.165 kb on + strand, within ABZR87_RS01080at 218.165 kb on + strand, within ABZR87_RS01080at 218.165 kb on + strand, within ABZR87_RS01080at 218.165 kb on + strand, within ABZR87_RS01080at 218.165 kb on + strand, within ABZR87_RS01080at 218.166 kb on - strand, within ABZR87_RS01080at 218.166 kb on - strand, within ABZR87_RS01080at 218.166 kb on - strand, within ABZR87_RS01080at 218.166 kb on - strand, within ABZR87_RS01080at 218.166 kb on - strand, within ABZR87_RS01080at 218.583 kb on + strand, within ABZR87_RS01080at 218.603 kb on + strand, within ABZR87_RS01080at 218.604 kb on - strand, within ABZR87_RS01080at 218.604 kb on - strand, within ABZR87_RS01080at 218.715 kb on + strand, within ABZR87_RS01080at 218.715 kb on + strand, within ABZR87_RS01080at 218.715 kb on + strand, within ABZR87_RS01080at 218.715 kb on + strand, within ABZR87_RS01080at 218.716 kb on - strand, within ABZR87_RS01080at 218.716 kb on - strand, within ABZR87_RS01080at 218.716 kb on - strand, within ABZR87_RS01080at 218.716 kb on - strand, within ABZR87_RS01080at 218.718 kb on + strand, within ABZR87_RS01080at 218.718 kb on + strand, within ABZR87_RS01080at 218.718 kb on + strand, within ABZR87_RS01080at 218.718 kb on + strand, within ABZR87_RS01080at 218.718 kb on + strand, within ABZR87_RS01080at 218.719 kb on - strand, within ABZR87_RS01080at 218.719 kb on - strand, within ABZR87_RS01080at 218.719 kb on - strand, within ABZR87_RS01080at 218.719 kb on - strand, within ABZR87_RS01080at 218.859 kb on - strand, within ABZR87_RS01080at 218.977 kb on + strand, within ABZR87_RS01080at 218.999 kb on + strand, within ABZR87_RS01080at 219.000 kb on - strand, within ABZR87_RS01080at 219.026 kb on + strand, within ABZR87_RS01080at 219.090 kb on - strand, within ABZR87_RS01080at 219.117 kb on + strand, within ABZR87_RS01080at 219.118 kb on - strand, within ABZR87_RS01080at 219.118 kb on - strand, within ABZR87_RS01080at 219.118 kb on - strand, within ABZR87_RS01080at 219.118 kb on - strand, within ABZR87_RS01080at 219.153 kb on + strand, within ABZR87_RS01080at 219.153 kb on + strand, within ABZR87_RS01080at 219.153 kb on + strand, within ABZR87_RS01080at 219.154 kb on - strand, within ABZR87_RS01080at 219.408 kb on - strandat 219.408 kb on - strandat 219.589 kb on + strand, within ABZR87_RS01085at 219.589 kb on + strand, within ABZR87_RS01085at 219.589 kb on + strand, within ABZR87_RS01085at 219.590 kb on - strand, within ABZR87_RS01085at 220.011 kb on - strand, within ABZR87_RS01085at 220.011 kb on - strand, within ABZR87_RS01085at 220.026 kb on - strand, within ABZR87_RS01085at 220.178 kb on + strandat 220.178 kb on + strandat 220.179 kb on - strandat 220.179 kb on - strandat 220.250 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB
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216,584 - ABZR87_RS01075 0.67 -0.5
216,747 + ABZR87_RS01075 0.77 +0.9
216,748 - ABZR87_RS01075 0.77 -1.1
216,937 + ABZR87_RS01075 0.89 +0.6
216,937 + ABZR87_RS01075 0.89 +1.2
216,937 + ABZR87_RS01075 0.89 +0.4
216,937 + ABZR87_RS01075 0.89 +1.5
216,938 - ABZR87_RS01075 0.89 +0.1
216,938 - ABZR87_RS01075 0.89 +1.1
216,938 - ABZR87_RS01075 0.89 -0.8
217,014 + +2.2
217,088 + +0.5
217,131 + -0.5
217,131 + -0.4
217,131 + +0.2
217,132 - +0.3
217,132 - -0.7
217,132 - +0.2
217,132 - +0.9
217,221 + +0.9
217,222 - +0.8
217,222 - -1.3
217,243 + -1.2
217,244 - +2.6
217,244 - +0.8
217,252 + +0.2
217,252 + -0.4
217,253 - +0.3
217,256 + -2.5
217,407 + +0.6
217,407 + -0.1
217,407 + -0.0
217,408 - +0.0
217,408 - -0.3
217,408 - -1.4
217,487 + -0.4
217,487 + -0.5
217,602 + ABZR87_RS01080 0.12 +0.5
217,602 + ABZR87_RS01080 0.12 +0.3
217,602 + ABZR87_RS01080 0.12 +1.6
217,602 + ABZR87_RS01080 0.12 -1.6
217,602 + ABZR87_RS01080 0.12 +0.4
217,603 - ABZR87_RS01080 0.12 -0.1
217,603 - ABZR87_RS01080 0.12 -1.2
217,646 + ABZR87_RS01080 0.15 +2.3
217,713 + ABZR87_RS01080 0.18 +0.6
217,713 + ABZR87_RS01080 0.18 +1.1
217,714 - ABZR87_RS01080 0.18 +0.4
217,714 - ABZR87_RS01080 0.18 +1.4
217,714 - ABZR87_RS01080 0.18 +1.2
217,714 - ABZR87_RS01080 0.18 +0.3
217,844 + ABZR87_RS01080 0.24 -0.7
217,845 - ABZR87_RS01080 0.24 -0.4
217,845 - ABZR87_RS01080 0.24 +0.9
217,862 + ABZR87_RS01080 0.25 +0.5
217,862 + ABZR87_RS01080 0.25 +2.3
217,862 + ABZR87_RS01080 0.25 +0.5
217,947 - ABZR87_RS01080 0.29 +0.1
217,984 - ABZR87_RS01080 0.31 -2.6
218,075 + ABZR87_RS01080 0.35 -2.5
218,075 + ABZR87_RS01080 0.35 +0.1
218,076 - ABZR87_RS01080 0.35 +1.3
218,076 - ABZR87_RS01080 0.35 +1.2
218,076 - ABZR87_RS01080 0.35 -0.2
218,125 - ABZR87_RS01080 0.38 -2.6
218,133 + ABZR87_RS01080 0.38 +0.2
218,134 - ABZR87_RS01080 0.38 -0.8
218,134 - ABZR87_RS01080 0.38 -1.1
218,165 + ABZR87_RS01080 0.40 +0.0
218,165 + ABZR87_RS01080 0.40 +0.8
218,165 + ABZR87_RS01080 0.40 +0.5
218,165 + ABZR87_RS01080 0.40 -0.2
218,165 + ABZR87_RS01080 0.40 +0.6
218,166 - ABZR87_RS01080 0.40 +1.5
218,166 - ABZR87_RS01080 0.40 -0.3
218,166 - ABZR87_RS01080 0.40 -0.1
218,166 - ABZR87_RS01080 0.40 +0.0
218,166 - ABZR87_RS01080 0.40 -1.1
218,583 + ABZR87_RS01080 0.60 +1.2
218,603 + ABZR87_RS01080 0.61 +1.1
218,604 - ABZR87_RS01080 0.61 +0.1
218,604 - ABZR87_RS01080 0.61 +0.2
218,715 + ABZR87_RS01080 0.67 +1.0
218,715 + ABZR87_RS01080 0.67 +0.7
218,715 + ABZR87_RS01080 0.67 +0.5
218,715 + ABZR87_RS01080 0.67 +0.2
218,716 - ABZR87_RS01080 0.67 -1.8
218,716 - ABZR87_RS01080 0.67 +0.9
218,716 - ABZR87_RS01080 0.67 +1.0
218,716 - ABZR87_RS01080 0.67 -1.5
218,718 + ABZR87_RS01080 0.67 -1.8
218,718 + ABZR87_RS01080 0.67 -0.0
218,718 + ABZR87_RS01080 0.67 -2.0
218,718 + ABZR87_RS01080 0.67 -0.9
218,718 + ABZR87_RS01080 0.67 -0.3
218,719 - ABZR87_RS01080 0.67 +1.0
218,719 - ABZR87_RS01080 0.67 +1.6
218,719 - ABZR87_RS01080 0.67 -0.4
218,719 - ABZR87_RS01080 0.67 -0.3
218,859 - ABZR87_RS01080 0.74 -0.5
218,977 + ABZR87_RS01080 0.79 +0.9
218,999 + ABZR87_RS01080 0.80 -0.3
219,000 - ABZR87_RS01080 0.80 -0.1
219,026 + ABZR87_RS01080 0.82 +0.5
219,090 - ABZR87_RS01080 0.85 -1.0
219,117 + ABZR87_RS01080 0.86 +0.8
219,118 - ABZR87_RS01080 0.86 -0.6
219,118 - ABZR87_RS01080 0.86 +0.1
219,118 - ABZR87_RS01080 0.86 +0.6
219,118 - ABZR87_RS01080 0.86 +0.1
219,153 + ABZR87_RS01080 0.88 +1.0
219,153 + ABZR87_RS01080 0.88 +1.6
219,153 + ABZR87_RS01080 0.88 -1.1
219,154 - ABZR87_RS01080 0.88 -2.3
219,408 - +0.5
219,408 - +0.4
219,589 + ABZR87_RS01085 0.15 -1.2
219,589 + ABZR87_RS01085 0.15 -2.2
219,589 + ABZR87_RS01085 0.15 -1.2
219,590 - ABZR87_RS01085 0.15 -3.4
220,011 - ABZR87_RS01085 0.83 -4.2
220,011 - ABZR87_RS01085 0.83 -1.8
220,026 - ABZR87_RS01085 0.85 -3.3
220,178 + -3.6
220,178 + -0.6
220,179 - -1.1
220,179 - +0.9
220,250 - -1.8

Or see this region's nucleotide sequence