Experiment: root EcoFAB sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00935 and ABZR87_RS00940 overlap by 4 nucleotides ABZR87_RS00940 and ABZR87_RS00945 are separated by 29 nucleotides ABZR87_RS00945 and ABZR87_RS00950 are separated by 60 nucleotides ABZR87_RS00950 and ABZR87_RS00955 are separated by 11 nucleotides
ABZR87_RS00935: ABZR87_RS00935 - polynucleotide adenylyltransferase PcnB, at 185,894 to 187,456
_RS00935
ABZR87_RS00940: ABZR87_RS00940 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 187,453 to 187,968
_RS00940
ABZR87_RS00945: ABZR87_RS00945 - deoxynucleoside kinase, at 187,998 to 188,642
_RS00945
ABZR87_RS00950: ABZR87_RS00950 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 188,703 to 189,521
_RS00950
ABZR87_RS00955: ABZR87_RS00955 - ABC transporter permease, at 189,533 to 190,240
_RS00955
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 187.048 kb on + strand, within ABZR87_RS00935 at 187.049 kb on - strand, within ABZR87_RS00935 at 187.049 kb on - strand, within ABZR87_RS00935 at 187.211 kb on + strand, within ABZR87_RS00935 at 187.211 kb on + strand, within ABZR87_RS00935 at 187.211 kb on + strand, within ABZR87_RS00935 at 187.212 kb on - strand, within ABZR87_RS00935 at 187.468 kb on + strand at 188.142 kb on + strand, within ABZR87_RS00945 at 188.142 kb on + strand, within ABZR87_RS00945 at 188.143 kb on - strand, within ABZR87_RS00945 at 188.143 kb on - strand, within ABZR87_RS00945 at 188.160 kb on + strand, within ABZR87_RS00945 at 188.322 kb on + strand, within ABZR87_RS00945 at 188.322 kb on + strand, within ABZR87_RS00945 at 188.322 kb on + strand, within ABZR87_RS00945 at 188.322 kb on + strand, within ABZR87_RS00945 at 188.322 kb on + strand, within ABZR87_RS00945 at 188.323 kb on - strand, within ABZR87_RS00945 at 188.323 kb on - strand, within ABZR87_RS00945 at 188.436 kb on + strand, within ABZR87_RS00945 at 188.436 kb on + strand, within ABZR87_RS00945 at 188.436 kb on + strand, within ABZR87_RS00945 at 188.460 kb on + strand, within ABZR87_RS00945 at 188.460 kb on + strand, within ABZR87_RS00945 at 188.461 kb on - strand, within ABZR87_RS00945 at 188.461 kb on - strand, within ABZR87_RS00945 at 188.502 kb on + strand, within ABZR87_RS00945 at 188.502 kb on + strand, within ABZR87_RS00945 at 188.502 kb on + strand, within ABZR87_RS00945 at 188.503 kb on - strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.505 kb on + strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.506 kb on - strand, within ABZR87_RS00945 at 188.709 kb on + strand at 188.709 kb on + strand at 188.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 1; outgrowth in LB remove 187,048 + ABZR87_RS00935 0.74 -2.3 187,049 - ABZR87_RS00935 0.74 -1.2 187,049 - ABZR87_RS00935 0.74 -0.5 187,211 + ABZR87_RS00935 0.84 -1.2 187,211 + ABZR87_RS00935 0.84 -0.2 187,211 + ABZR87_RS00935 0.84 -2.1 187,212 - ABZR87_RS00935 0.84 -0.9 187,468 + -0.3 188,142 + ABZR87_RS00945 0.22 +0.4 188,142 + ABZR87_RS00945 0.22 +0.6 188,143 - ABZR87_RS00945 0.22 -0.3 188,143 - ABZR87_RS00945 0.22 -0.4 188,160 + ABZR87_RS00945 0.25 -0.1 188,322 + ABZR87_RS00945 0.50 -1.6 188,322 + ABZR87_RS00945 0.50 +0.3 188,322 + ABZR87_RS00945 0.50 -2.8 188,322 + ABZR87_RS00945 0.50 -0.5 188,322 + ABZR87_RS00945 0.50 +0.4 188,323 - ABZR87_RS00945 0.50 +1.2 188,323 - ABZR87_RS00945 0.50 -0.0 188,436 + ABZR87_RS00945 0.68 +0.9 188,436 + ABZR87_RS00945 0.68 +0.0 188,436 + ABZR87_RS00945 0.68 +0.6 188,460 + ABZR87_RS00945 0.72 +0.4 188,460 + ABZR87_RS00945 0.72 +2.3 188,461 - ABZR87_RS00945 0.72 -0.8 188,461 - ABZR87_RS00945 0.72 +0.1 188,502 + ABZR87_RS00945 0.78 +2.3 188,502 + ABZR87_RS00945 0.78 -0.1 188,502 + ABZR87_RS00945 0.78 +0.2 188,503 - ABZR87_RS00945 0.78 +1.2 188,505 + ABZR87_RS00945 0.79 +0.1 188,505 + ABZR87_RS00945 0.79 +0.4 188,505 + ABZR87_RS00945 0.79 +0.8 188,505 + ABZR87_RS00945 0.79 -1.3 188,505 + ABZR87_RS00945 0.79 +0.0 188,505 + ABZR87_RS00945 0.79 +0.5 188,506 - ABZR87_RS00945 0.79 +0.5 188,506 - ABZR87_RS00945 0.79 +0.9 188,506 - ABZR87_RS00945 0.79 -1.3 188,506 - ABZR87_RS00945 0.79 +0.0 188,506 - ABZR87_RS00945 0.79 -1.3 188,506 - ABZR87_RS00945 0.79 -1.4 188,709 + -1.8 188,709 + -0.2 188,709 + +0.3
Or see this region's nucleotide sequence