Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1800 and Shew_1801 are separated by 269 nucleotides Shew_1801 and Shew_1802 are separated by 31 nucleotides Shew_1802 and Shew_1803 are separated by 72 nucleotides
Shew_1800: Shew_1800 - putative GAF sensor protein (RefSeq), at 2,060,893 to 2,061,351
_1800
Shew_1801: Shew_1801 - putative solute/DNA competence effector (RefSeq), at 2,061,621 to 2,062,268
_1801
Shew_1802: Shew_1802 - carboxy-terminal protease (RefSeq), at 2,062,300 to 2,064,345
_1802
Shew_1803: Shew_1803 - aminopeptidase N (RefSeq), at 2,064,418 to 2,066,979
_1803
Position (kb)
2062
2063
2064
2065 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2061.497 kb on + strand at 2061.591 kb on + strand at 2061.840 kb on - strand, within Shew_1801 at 2061.842 kb on + strand, within Shew_1801 at 2062.525 kb on - strand, within Shew_1802 at 2062.678 kb on + strand, within Shew_1802 at 2063.025 kb on + strand, within Shew_1802 at 2063.561 kb on + strand, within Shew_1802 at 2063.868 kb on + strand, within Shew_1802 at 2063.876 kb on - strand, within Shew_1802 at 2064.566 kb on + strand at 2064.566 kb on + strand at 2064.574 kb on - strand at 2064.574 kb on - strand at 2064.768 kb on + strand, within Shew_1803 at 2065.079 kb on - strand, within Shew_1803 at 2065.263 kb on + strand, within Shew_1803 at 2065.299 kb on - strand, within Shew_1803
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,061,497 + -1.3 2,061,591 + -0.9 2,061,840 - Shew_1801 0.34 -1.4 2,061,842 + Shew_1801 0.34 -1.2 2,062,525 - Shew_1802 0.11 -0.1 2,062,678 + Shew_1802 0.18 -1.7 2,063,025 + Shew_1802 0.35 -0.3 2,063,561 + Shew_1802 0.62 -0.8 2,063,868 + Shew_1802 0.77 -0.4 2,063,876 - Shew_1802 0.77 -3.8 2,064,566 + +0.3 2,064,566 + +0.3 2,064,574 - -0.3 2,064,574 - -0.0 2,064,768 + Shew_1803 0.14 -0.0 2,065,079 - Shew_1803 0.26 -0.0 2,065,263 + Shew_1803 0.33 -0.3 2,065,299 - Shew_1803 0.34 -1.7
Or see this region's nucleotide sequence