Experiment: H2 N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04015 and MMJJ_RS04020 are separated by 29 nucleotides MMJJ_RS04020 and MMJJ_RS04025 are separated by 54 nucleotides MMJJ_RS04025 and MMJJ_RS04030 overlap by 1 nucleotides MMJJ_RS04030 and MMJJ_RS04035 are separated by 7 nucleotides MMJJ_RS04035 and MMJJ_RS04040 are separated by 57 nucleotides MMJJ_RS04040 and MMJJ_RS04045 are separated by 37 nucleotides
MMJJ_RS04015: MMJJ_RS04015 - ribosome assembly factor SBDS, at 749,627 to 750,331
_RS04015
MMJJ_RS04020: MMJJ_RS04020 - 50S ribosomal protein L37Ae, at 750,361 to 750,651
_RS04020
MMJJ_RS04025: MMJJ_RS04025 - DNA-directed RNA polymerase subunit P, at 750,706 to 750,846
_RS04025
MMJJ_RS04030: MMJJ_RS04030 - rRNA maturation protein, at 750,846 to 751,340
_RS04030
MMJJ_RS04035: MMJJ_RS04035 - hypothetical protein, at 751,348 to 751,599
_RS04035
MMJJ_RS04040: MMJJ_RS04040 - prefoldin subunit beta, at 751,657 to 751,998
_RS04040
MMJJ_RS04045: MMJJ_RS04045 - DHH family phosphoesterase, at 752,036 to 753,052
_RS04045
Position (kb) 750
751
752 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 750.323 kb on - strand at 750.603 kb on - strand, within MMJJ_RS04020 at 750.810 kb on + strand, within MMJJ_RS04025 at 750.981 kb on + strand, within MMJJ_RS04030 at 751.200 kb on - strand, within MMJJ_RS04030 at 751.230 kb on + strand, within MMJJ_RS04030 at 751.310 kb on + strand at 751.573 kb on + strand, within MMJJ_RS04035 at 751.574 kb on - strand at 751.575 kb on + strand at 751.575 kb on + strand at 751.575 kb on + strand at 751.576 kb on - strand at 751.577 kb on + strand at 751.577 kb on + strand at 751.578 kb on - strand at 751.578 kb on - strand at 751.579 kb on + strand at 751.598 kb on + strand at 751.600 kb on - strand at 751.601 kb on + strand at 751.633 kb on - strand at 751.633 kb on - strand at 751.727 kb on - strand, within MMJJ_RS04040 at 751.728 kb on + strand, within MMJJ_RS04040 at 751.819 kb on + strand, within MMJJ_RS04040 at 751.819 kb on + strand, within MMJJ_RS04040 at 751.887 kb on + strand, within MMJJ_RS04040 at 751.908 kb on + strand, within MMJJ_RS04040 at 751.908 kb on + strand, within MMJJ_RS04040 at 751.911 kb on - strand, within MMJJ_RS04040 at 751.911 kb on - strand, within MMJJ_RS04040 at 751.912 kb on + strand, within MMJJ_RS04040 at 751.912 kb on + strand, within MMJJ_RS04040 at 751.912 kb on + strand, within MMJJ_RS04040 at 751.949 kb on + strand, within MMJJ_RS04040 at 751.958 kb on - strand, within MMJJ_RS04040 at 751.980 kb on + strand at 752.013 kb on + strand at 752.014 kb on - strand at 752.087 kb on + strand at 752.088 kb on - strand at 752.089 kb on + strand at 752.094 kb on - strand at 752.301 kb on - strand, within MMJJ_RS04045 at 752.320 kb on + strand, within MMJJ_RS04045
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2mM Adenosine remove 750,323 - +0.3 750,603 - MMJJ_RS04020 0.83 +1.3 750,810 + MMJJ_RS04025 0.74 -1.1 750,981 + MMJJ_RS04030 0.27 +0.7 751,200 - MMJJ_RS04030 0.72 +3.3 751,230 + MMJJ_RS04030 0.78 +2.3 751,310 + +2.0 751,573 + MMJJ_RS04035 0.89 -1.1 751,574 - -1.6 751,575 + -2.5 751,575 + +1.2 751,575 + +1.2 751,576 - -0.7 751,577 + -2.6 751,577 + -1.5 751,578 - -2.8 751,578 - -1.8 751,579 + +0.9 751,598 + -1.3 751,600 - -3.2 751,601 + -2.5 751,633 - -3.9 751,633 - -2.8 751,727 - MMJJ_RS04040 0.20 -2.8 751,728 + MMJJ_RS04040 0.21 -2.3 751,819 + MMJJ_RS04040 0.47 -2.9 751,819 + MMJJ_RS04040 0.47 -2.8 751,887 + MMJJ_RS04040 0.67 -0.4 751,908 + MMJJ_RS04040 0.73 -3.1 751,908 + MMJJ_RS04040 0.73 -1.9 751,911 - MMJJ_RS04040 0.74 -0.7 751,911 - MMJJ_RS04040 0.74 -0.7 751,912 + MMJJ_RS04040 0.75 -2.1 751,912 + MMJJ_RS04040 0.75 -1.9 751,912 + MMJJ_RS04040 0.75 -3.0 751,949 + MMJJ_RS04040 0.85 -1.2 751,958 - MMJJ_RS04040 0.88 -1.1 751,980 + -1.2 752,013 + +1.4 752,014 - -1.9 752,087 + +0.7 752,088 - -0.9 752,089 + -2.6 752,094 - +0.4 752,301 - MMJJ_RS04045 0.26 -1.2 752,320 + MMJJ_RS04045 0.28 -0.8
Or see this region's nucleotide sequence