Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30605 and AZOBR_RS30610 are separated by 112 nucleotides AZOBR_RS30610 and AZOBR_RS30615 are separated by 98 nucleotides
AZOBR_RS30605: AZOBR_RS30605 - poly-beta-hydroxybutyrate polymerase, at 222,559 to 223,797
_RS30605
AZOBR_RS30610: AZOBR_RS30610 - acetyl-CoA acetyltransferase, at 223,910 to 225,085
_RS30610
AZOBR_RS30615: AZOBR_RS30615 - acetoacetyl-CoA reductase, at 225,184 to 225,906
_RS30615
Position (kb) 223
224
225
226 Strain fitness (log2 ratio) -1
0
1 at 222.973 kb on + strand, within AZOBR_RS30605 at 222.973 kb on + strand, within AZOBR_RS30605 at 222.974 kb on - strand, within AZOBR_RS30605 at 222.974 kb on - strand, within AZOBR_RS30605 at 223.096 kb on + strand, within AZOBR_RS30605 at 223.097 kb on - strand, within AZOBR_RS30605 at 223.535 kb on - strand, within AZOBR_RS30605 at 223.642 kb on - strand, within AZOBR_RS30605 at 223.720 kb on - strand at 223.720 kb on - strand at 223.720 kb on - strand at 223.942 kb on + strand at 223.991 kb on + strand at 224.370 kb on - strand, within AZOBR_RS30610 at 224.993 kb on + strand at 224.994 kb on - strand at 224.994 kb on - strand at 225.126 kb on + strand at 225.127 kb on - strand at 225.127 kb on - strand at 225.127 kb on - strand at 225.262 kb on + strand, within AZOBR_RS30615 at 225.263 kb on - strand, within AZOBR_RS30615 at 225.281 kb on - strand, within AZOBR_RS30615 at 225.337 kb on + strand, within AZOBR_RS30615 at 225.338 kb on - strand, within AZOBR_RS30615 at 225.608 kb on - strand at 225.622 kb on + strand, within AZOBR_RS30615 at 225.623 kb on - strand, within AZOBR_RS30615 at 225.718 kb on + strand at 225.718 kb on + strand, within AZOBR_RS30615 at 225.901 kb on + strand at 225.901 kb on + strand at 225.902 kb on - strand at 225.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 222,973 + AZOBR_RS30605 0.33 +1.7 222,973 + AZOBR_RS30605 0.33 -1.1 222,974 - AZOBR_RS30605 0.33 -0.1 222,974 - AZOBR_RS30605 0.33 -0.2 223,096 + AZOBR_RS30605 0.43 -0.5 223,097 - AZOBR_RS30605 0.43 +0.6 223,535 - AZOBR_RS30605 0.79 +1.4 223,642 - AZOBR_RS30605 0.87 -0.6 223,720 - -0.4 223,720 - -0.3 223,720 - +1.7 223,942 + -0.1 223,991 + -1.1 224,370 - AZOBR_RS30610 0.39 -1.2 224,993 + -0.3 224,994 - -0.7 224,994 - -0.5 225,126 + -1.4 225,127 - +1.0 225,127 - -0.3 225,127 - -0.0 225,262 + AZOBR_RS30615 0.11 -0.5 225,263 - AZOBR_RS30615 0.11 -0.4 225,281 - AZOBR_RS30615 0.13 -0.6 225,337 + AZOBR_RS30615 0.21 -0.6 225,338 - AZOBR_RS30615 0.21 +0.1 225,608 - -0.4 225,622 + AZOBR_RS30615 0.61 -1.4 225,623 - AZOBR_RS30615 0.61 -1.6 225,718 + +1.2 225,718 + AZOBR_RS30615 0.74 -0.1 225,901 + -0.1 225,901 + -0.0 225,902 - +0.7 225,902 - -0.4
Or see this region's nucleotide sequence