Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS23305 and AZOBR_RS23310 are separated by 35 nucleotides AZOBR_RS23310 and AZOBR_RS23315 are separated by 53 nucleotides AZOBR_RS23315 and AZOBR_RS23320 overlap by 7 nucleotides
AZOBR_RS23305: AZOBR_RS23305 - hypothetical protein, at 254,386 to 255,366
_RS23305
AZOBR_RS23310: AZOBR_RS23310 - hypothetical protein, at 255,402 to 256,379
_RS23310
AZOBR_RS23315: AZOBR_RS23315 - conserved membrane protein of unknown function, at 256,433 to 256,918
_RS23315
AZOBR_RS23320: AZOBR_RS23320 - C4-dicarboxylate ABC transporter permease, at 256,912 to 258,414
_RS23320
Position (kb) 255
256
257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 254.531 kb on - strand, within AZOBR_RS23305 at 254.531 kb on - strand, within AZOBR_RS23305 at 254.632 kb on + strand, within AZOBR_RS23305 at 254.632 kb on + strand, within AZOBR_RS23305 at 254.632 kb on + strand, within AZOBR_RS23305 at 254.632 kb on + strand, within AZOBR_RS23305 at 254.632 kb on + strand, within AZOBR_RS23305 at 254.633 kb on - strand, within AZOBR_RS23305 at 254.633 kb on - strand, within AZOBR_RS23305 at 254.634 kb on + strand, within AZOBR_RS23305 at 254.634 kb on + strand, within AZOBR_RS23305 at 254.634 kb on + strand, within AZOBR_RS23305 at 254.634 kb on + strand, within AZOBR_RS23305 at 254.635 kb on - strand, within AZOBR_RS23305 at 254.690 kb on - strand, within AZOBR_RS23305 at 254.690 kb on - strand, within AZOBR_RS23305 at 254.728 kb on + strand, within AZOBR_RS23305 at 254.728 kb on + strand, within AZOBR_RS23305 at 254.729 kb on - strand, within AZOBR_RS23305 at 254.729 kb on - strand, within AZOBR_RS23305 at 255.092 kb on - strand, within AZOBR_RS23305 at 255.134 kb on - strand, within AZOBR_RS23305 at 255.355 kb on + strand at 255.356 kb on - strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.364 kb on + strand at 255.368 kb on + strand at 255.369 kb on - strand at 255.703 kb on - strand, within AZOBR_RS23310 at 255.703 kb on - strand, within AZOBR_RS23310 at 256.252 kb on - strand, within AZOBR_RS23310 at 256.252 kb on - strand, within AZOBR_RS23310 at 256.291 kb on - strand at 256.496 kb on + strand, within AZOBR_RS23315 at 256.709 kb on + strand, within AZOBR_RS23315 at 256.709 kb on + strand, within AZOBR_RS23315 at 256.710 kb on - strand, within AZOBR_RS23315 at 256.710 kb on - strand, within AZOBR_RS23315 at 256.867 kb on - strand, within AZOBR_RS23315 at 256.867 kb on - strand, within AZOBR_RS23315 at 256.867 kb on - strand, within AZOBR_RS23315 at 256.911 kb on + strand at 256.911 kb on + strand at 256.912 kb on - strand at 256.922 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.987 kb on + strand at 256.988 kb on - strand at 257.179 kb on + strand, within AZOBR_RS23320 at 257.179 kb on + strand, within AZOBR_RS23320 at 257.179 kb on + strand, within AZOBR_RS23320
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 254,531 - AZOBR_RS23305 0.15 +2.0 254,531 - AZOBR_RS23305 0.15 +0.4 254,632 + AZOBR_RS23305 0.25 +1.2 254,632 + AZOBR_RS23305 0.25 -0.7 254,632 + AZOBR_RS23305 0.25 -0.3 254,632 + AZOBR_RS23305 0.25 -2.9 254,632 + AZOBR_RS23305 0.25 -0.3 254,633 - AZOBR_RS23305 0.25 -0.1 254,633 - AZOBR_RS23305 0.25 -0.0 254,634 + AZOBR_RS23305 0.25 +0.0 254,634 + AZOBR_RS23305 0.25 -2.5 254,634 + AZOBR_RS23305 0.25 +0.3 254,634 + AZOBR_RS23305 0.25 -1.1 254,635 - AZOBR_RS23305 0.25 +0.8 254,690 - AZOBR_RS23305 0.31 -0.2 254,690 - AZOBR_RS23305 0.31 +0.2 254,728 + AZOBR_RS23305 0.35 -0.2 254,728 + AZOBR_RS23305 0.35 +1.8 254,729 - AZOBR_RS23305 0.35 -0.2 254,729 - AZOBR_RS23305 0.35 -0.8 255,092 - AZOBR_RS23305 0.72 +0.3 255,134 - AZOBR_RS23305 0.76 +0.2 255,355 + +0.8 255,356 - +0.7 255,364 + +2.5 255,364 + -4.3 255,364 + +0.1 255,364 + -0.7 255,364 + +1.0 255,364 + -3.6 255,364 + +0.5 255,364 + +0.1 255,364 + +0.0 255,368 + +1.2 255,369 - -3.2 255,703 - AZOBR_RS23310 0.31 -2.1 255,703 - AZOBR_RS23310 0.31 +1.3 256,252 - AZOBR_RS23310 0.87 +1.8 256,252 - AZOBR_RS23310 0.87 +3.2 256,291 - -0.7 256,496 + AZOBR_RS23315 0.13 -1.2 256,709 + AZOBR_RS23315 0.57 -0.3 256,709 + AZOBR_RS23315 0.57 -0.8 256,710 - AZOBR_RS23315 0.57 +0.3 256,710 - AZOBR_RS23315 0.57 -1.3 256,867 - AZOBR_RS23315 0.89 -0.7 256,867 - AZOBR_RS23315 0.89 +0.1 256,867 - AZOBR_RS23315 0.89 -0.2 256,911 + -1.1 256,911 + +0.2 256,912 - -1.3 256,922 + -0.6 256,987 + -0.4 256,987 + +0.4 256,987 + -0.2 256,987 + -0.5 256,987 + -1.9 256,987 + +0.7 256,987 + -0.2 256,988 - -1.6 257,179 + AZOBR_RS23320 0.18 -0.1 257,179 + AZOBR_RS23320 0.18 +0.1 257,179 + AZOBR_RS23320 0.18 -0.2
Or see this region's nucleotide sequence