Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS18215 and AZOBR_RS18220 are separated by 196 nucleotides AZOBR_RS18220 and AZOBR_RS18225 are separated by 14 nucleotides AZOBR_RS18225 and AZOBR_RS18230 are separated by 291 nucleotides
AZOBR_RS18215: AZOBR_RS18215 - hypothetical protein, at 849,753 to 851,039
_RS18215
AZOBR_RS18220: AZOBR_RS18220 - conserved membrane protein of unknown function, at 851,236 to 854,322
_RS18220
AZOBR_RS18225: AZOBR_RS18225 - hypothetical protein, at 854,337 to 854,720
_RS18225
AZOBR_RS18230: AZOBR_RS18230 - hypothetical protein, at 855,012 to 857,963
_RS18230
Position (kb) 851
852
853
854
855 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 850.488 kb on + strand, within AZOBR_RS18215 at 850.737 kb on + strand, within AZOBR_RS18215 at 850.737 kb on + strand, within AZOBR_RS18215 at 850.737 kb on + strand, within AZOBR_RS18215 at 850.863 kb on + strand, within AZOBR_RS18215 at 850.863 kb on + strand, within AZOBR_RS18215 at 850.866 kb on + strand, within AZOBR_RS18215 at 850.866 kb on + strand, within AZOBR_RS18215 at 850.866 kb on + strand, within AZOBR_RS18215 at 850.866 kb on + strand, within AZOBR_RS18215 at 850.866 kb on + strand, within AZOBR_RS18215 at 850.867 kb on - strand, within AZOBR_RS18215 at 850.867 kb on - strand, within AZOBR_RS18215 at 850.867 kb on - strand, within AZOBR_RS18215 at 850.867 kb on - strand, within AZOBR_RS18215 at 850.906 kb on - strand, within AZOBR_RS18215 at 850.920 kb on + strand at 850.920 kb on + strand at 850.921 kb on - strand at 850.986 kb on + strand at 850.986 kb on + strand at 850.986 kb on + strand at 850.986 kb on + strand at 850.986 kb on + strand at 850.986 kb on + strand at 850.987 kb on - strand at 850.987 kb on - strand at 850.987 kb on - strand at 851.060 kb on - strand at 851.060 kb on - strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.183 kb on + strand at 851.657 kb on - strand, within AZOBR_RS18220 at 851.782 kb on + strand, within AZOBR_RS18220 at 851.782 kb on + strand, within AZOBR_RS18220 at 851.782 kb on + strand, within AZOBR_RS18220 at 851.782 kb on + strand, within AZOBR_RS18220 at 851.783 kb on - strand, within AZOBR_RS18220 at 852.238 kb on + strand, within AZOBR_RS18220 at 852.238 kb on + strand, within AZOBR_RS18220 at 852.238 kb on + strand, within AZOBR_RS18220 at 852.239 kb on - strand, within AZOBR_RS18220 at 852.475 kb on + strand, within AZOBR_RS18220 at 852.577 kb on + strand, within AZOBR_RS18220 at 852.598 kb on + strand, within AZOBR_RS18220 at 852.598 kb on + strand, within AZOBR_RS18220 at 852.599 kb on - strand, within AZOBR_RS18220 at 852.599 kb on - strand, within AZOBR_RS18220 at 852.599 kb on - strand, within AZOBR_RS18220 at 852.617 kb on - strand, within AZOBR_RS18220 at 852.617 kb on - strand, within AZOBR_RS18220 at 852.617 kb on - strand, within AZOBR_RS18220 at 852.803 kb on - strand, within AZOBR_RS18220 at 852.803 kb on - strand, within AZOBR_RS18220 at 852.803 kb on - strand, within AZOBR_RS18220 at 852.862 kb on + strand, within AZOBR_RS18220 at 853.195 kb on + strand, within AZOBR_RS18220 at 853.195 kb on + strand, within AZOBR_RS18220 at 853.196 kb on - strand, within AZOBR_RS18220 at 853.306 kb on + strand, within AZOBR_RS18220 at 853.307 kb on - strand, within AZOBR_RS18220 at 853.307 kb on - strand, within AZOBR_RS18220 at 853.307 kb on - strand, within AZOBR_RS18220 at 853.984 kb on + strand, within AZOBR_RS18220 at 853.984 kb on + strand, within AZOBR_RS18220 at 853.987 kb on + strand, within AZOBR_RS18220 at 853.987 kb on + strand, within AZOBR_RS18220 at 853.988 kb on - strand, within AZOBR_RS18220 at 853.988 kb on - strand, within AZOBR_RS18220 at 853.988 kb on - strand, within AZOBR_RS18220 at 854.140 kb on + strand at 854.167 kb on + strand at 854.496 kb on + strand, within AZOBR_RS18225 at 854.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 850,488 + AZOBR_RS18215 0.57 +1.6 850,737 + AZOBR_RS18215 0.76 +0.0 850,737 + AZOBR_RS18215 0.76 +1.9 850,737 + AZOBR_RS18215 0.76 -1.0 850,863 + AZOBR_RS18215 0.86 -3.1 850,863 + AZOBR_RS18215 0.86 +0.2 850,866 + AZOBR_RS18215 0.86 -0.1 850,866 + AZOBR_RS18215 0.86 +0.7 850,866 + AZOBR_RS18215 0.86 +1.0 850,866 + AZOBR_RS18215 0.86 -0.5 850,866 + AZOBR_RS18215 0.86 -2.4 850,867 - AZOBR_RS18215 0.87 -0.7 850,867 - AZOBR_RS18215 0.87 -0.1 850,867 - AZOBR_RS18215 0.87 -0.5 850,867 - AZOBR_RS18215 0.87 -3.4 850,906 - AZOBR_RS18215 0.90 -1.7 850,920 + -1.4 850,920 + +1.0 850,921 - -1.4 850,986 + -2.4 850,986 + +0.3 850,986 + +0.8 850,986 + +0.5 850,986 + +5.0 850,986 + +2.1 850,987 - -2.5 850,987 - -1.5 850,987 - -1.6 851,060 - -0.3 851,060 - -0.3 851,183 + +0.5 851,183 + +1.7 851,183 + +2.1 851,183 + +0.5 851,183 + +0.5 851,183 + -2.7 851,183 + +4.3 851,183 + +0.4 851,657 - AZOBR_RS18220 0.14 -0.4 851,782 + AZOBR_RS18220 0.18 -2.3 851,782 + AZOBR_RS18220 0.18 -0.5 851,782 + AZOBR_RS18220 0.18 +2.7 851,782 + AZOBR_RS18220 0.18 +0.1 851,783 - AZOBR_RS18220 0.18 -0.4 852,238 + AZOBR_RS18220 0.32 -1.9 852,238 + AZOBR_RS18220 0.32 +0.3 852,238 + AZOBR_RS18220 0.32 -0.4 852,239 - AZOBR_RS18220 0.32 +0.5 852,475 + AZOBR_RS18220 0.40 -1.7 852,577 + AZOBR_RS18220 0.43 -1.9 852,598 + AZOBR_RS18220 0.44 -0.3 852,598 + AZOBR_RS18220 0.44 +0.9 852,599 - AZOBR_RS18220 0.44 +0.6 852,599 - AZOBR_RS18220 0.44 -1.0 852,599 - AZOBR_RS18220 0.44 -1.4 852,617 - AZOBR_RS18220 0.45 -0.8 852,617 - AZOBR_RS18220 0.45 -0.9 852,617 - AZOBR_RS18220 0.45 +0.5 852,803 - AZOBR_RS18220 0.51 -1.5 852,803 - AZOBR_RS18220 0.51 -0.5 852,803 - AZOBR_RS18220 0.51 -1.4 852,862 + AZOBR_RS18220 0.53 +2.0 853,195 + AZOBR_RS18220 0.63 +1.7 853,195 + AZOBR_RS18220 0.63 -1.8 853,196 - AZOBR_RS18220 0.63 -1.2 853,306 + AZOBR_RS18220 0.67 -3.2 853,307 - AZOBR_RS18220 0.67 -1.8 853,307 - AZOBR_RS18220 0.67 -1.7 853,307 - AZOBR_RS18220 0.67 +0.2 853,984 + AZOBR_RS18220 0.89 -0.0 853,984 + AZOBR_RS18220 0.89 -3.9 853,987 + AZOBR_RS18220 0.89 -1.0 853,987 + AZOBR_RS18220 0.89 -0.9 853,988 - AZOBR_RS18220 0.89 -0.3 853,988 - AZOBR_RS18220 0.89 +0.0 853,988 - AZOBR_RS18220 0.89 -0.4 854,140 + +0.1 854,167 + +1.2 854,496 + AZOBR_RS18225 0.41 +0.5 854,782 + -0.1
Or see this region's nucleotide sequence