Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05535 and AZOBR_RS05540 are separated by 4 nucleotides AZOBR_RS05540 and AZOBR_RS05545 are separated by 158 nucleotides AZOBR_RS05545 and AZOBR_RS05550 are separated by 14 nucleotides
AZOBR_RS05535: AZOBR_RS05535 - hypothetical protein, at 1,175,539 to 1,176,003
_RS05535
AZOBR_RS05540: AZOBR_RS05540 - nitrogen fixation protein NifX, at 1,176,008 to 1,176,409
_RS05540
AZOBR_RS05545: AZOBR_RS05545 - nitrogenase molybdenum-cofactor biosynthesis protein NifN, at 1,176,568 to 1,177,848
_RS05545
AZOBR_RS05550: AZOBR_RS05550 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 1,177,863 to 1,179,278
_RS05550
Position (kb)
1176
1177
1178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1175.786 kb on + strand, within AZOBR_RS05535 at 1175.786 kb on + strand, within AZOBR_RS05535 at 1175.786 kb on + strand, within AZOBR_RS05535 at 1175.787 kb on - strand, within AZOBR_RS05535 at 1175.787 kb on - strand, within AZOBR_RS05535 at 1175.787 kb on - strand, within AZOBR_RS05535 at 1175.915 kb on + strand, within AZOBR_RS05535 at 1175.916 kb on - strand, within AZOBR_RS05535 at 1176.207 kb on + strand, within AZOBR_RS05540 at 1176.333 kb on + strand, within AZOBR_RS05540 at 1176.334 kb on - strand, within AZOBR_RS05540 at 1177.506 kb on - strand, within AZOBR_RS05545 at 1177.996 kb on + strand at 1177.997 kb on - strand at 1178.122 kb on + strand, within AZOBR_RS05550 at 1178.122 kb on + strand, within AZOBR_RS05550 at 1178.123 kb on - strand, within AZOBR_RS05550 at 1178.123 kb on - strand, within AZOBR_RS05550 at 1178.123 kb on - strand, within AZOBR_RS05550 at 1178.123 kb on - strand, within AZOBR_RS05550 at 1178.123 kb on - strand, within AZOBR_RS05550 at 1178.326 kb on + strand, within AZOBR_RS05550 at 1178.326 kb on + strand, within AZOBR_RS05550 at 1178.327 kb on - strand, within AZOBR_RS05550 at 1178.327 kb on - strand, within AZOBR_RS05550 at 1178.467 kb on + strand, within AZOBR_RS05550 at 1178.467 kb on + strand, within AZOBR_RS05550 at 1178.468 kb on - strand, within AZOBR_RS05550 at 1178.590 kb on + strand, within AZOBR_RS05550 at 1178.590 kb on + strand, within AZOBR_RS05550 at 1178.591 kb on - strand, within AZOBR_RS05550 at 1178.681 kb on - strand, within AZOBR_RS05550
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 1,175,786 + AZOBR_RS05535 0.53 +0.3 1,175,786 + AZOBR_RS05535 0.53 +0.0 1,175,786 + AZOBR_RS05535 0.53 +0.1 1,175,787 - AZOBR_RS05535 0.53 -3.2 1,175,787 - AZOBR_RS05535 0.53 -1.3 1,175,787 - AZOBR_RS05535 0.53 -0.0 1,175,915 + AZOBR_RS05535 0.81 +0.0 1,175,916 - AZOBR_RS05535 0.81 -1.1 1,176,207 + AZOBR_RS05540 0.50 +2.8 1,176,333 + AZOBR_RS05540 0.81 -2.3 1,176,334 - AZOBR_RS05540 0.81 +0.4 1,177,506 - AZOBR_RS05545 0.73 +0.7 1,177,996 + -1.2 1,177,997 - -1.9 1,178,122 + AZOBR_RS05550 0.18 +0.5 1,178,122 + AZOBR_RS05550 0.18 -1.3 1,178,123 - AZOBR_RS05550 0.18 +1.2 1,178,123 - AZOBR_RS05550 0.18 -2.8 1,178,123 - AZOBR_RS05550 0.18 -0.5 1,178,123 - AZOBR_RS05550 0.18 +1.0 1,178,123 - AZOBR_RS05550 0.18 -1.3 1,178,326 + AZOBR_RS05550 0.33 +0.8 1,178,326 + AZOBR_RS05550 0.33 -0.3 1,178,327 - AZOBR_RS05550 0.33 +0.4 1,178,327 - AZOBR_RS05550 0.33 +0.3 1,178,467 + AZOBR_RS05550 0.43 -0.3 1,178,467 + AZOBR_RS05550 0.43 +0.3 1,178,468 - AZOBR_RS05550 0.43 +0.8 1,178,590 + AZOBR_RS05550 0.51 +0.7 1,178,590 + AZOBR_RS05550 0.51 +0.8 1,178,591 - AZOBR_RS05550 0.51 -1.2 1,178,681 - AZOBR_RS05550 0.58 -2.4
Or see this region's nucleotide sequence