Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01540 and AZOBR_RS01545 overlap by 4 nucleotides AZOBR_RS01545 and AZOBR_RS01550 are separated by 63 nucleotides AZOBR_RS01550 and AZOBR_RS01555 are separated by 177 nucleotides
AZOBR_RS01540: AZOBR_RS01540 - hypothetical protein, at 317,194 to 317,973
_RS01540
AZOBR_RS01545: AZOBR_RS01545 - permease, at 317,970 to 318,887
_RS01545
AZOBR_RS01550: AZOBR_RS01550 - carbohydrate kinase, at 318,951 to 319,949
_RS01550
AZOBR_RS01555: AZOBR_RS01555 - membrane protein, at 320,127 to 320,819
_RS01555
Position (kb) 318
319
320 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 318.275 kb on - strand, within AZOBR_RS01545 at 318.376 kb on + strand, within AZOBR_RS01545 at 318.535 kb on + strand, within AZOBR_RS01545 at 318.535 kb on + strand, within AZOBR_RS01545 at 318.535 kb on + strand, within AZOBR_RS01545 at 318.536 kb on - strand, within AZOBR_RS01545 at 318.579 kb on + strand, within AZOBR_RS01545 at 318.579 kb on + strand, within AZOBR_RS01545 at 318.580 kb on - strand, within AZOBR_RS01545 at 318.580 kb on - strand, within AZOBR_RS01545 at 318.878 kb on - strand at 318.878 kb on - strand at 318.924 kb on + strand at 318.924 kb on + strand at 318.925 kb on - strand at 318.925 kb on - strand at 318.925 kb on - strand at 318.925 kb on - strand at 318.925 kb on - strand at 318.926 kb on + strand at 318.927 kb on - strand at 318.931 kb on + strand at 318.931 kb on + strand at 318.932 kb on - strand at 318.932 kb on - strand at 318.952 kb on + strand at 318.975 kb on - strand at 318.975 kb on - strand at 319.108 kb on + strand, within AZOBR_RS01550 at 319.280 kb on - strand, within AZOBR_RS01550 at 319.471 kb on + strand, within AZOBR_RS01550 at 319.471 kb on + strand, within AZOBR_RS01550 at 319.472 kb on - strand, within AZOBR_RS01550 at 319.483 kb on + strand, within AZOBR_RS01550 at 319.484 kb on - strand, within AZOBR_RS01550 at 319.484 kb on - strand, within AZOBR_RS01550 at 319.484 kb on - strand, within AZOBR_RS01550 at 319.714 kb on + strand, within AZOBR_RS01550 at 319.715 kb on - strand, within AZOBR_RS01550 at 319.715 kb on - strand, within AZOBR_RS01550 at 319.804 kb on + strand, within AZOBR_RS01550 at 319.804 kb on + strand, within AZOBR_RS01550 at 320.015 kb on - strand at 320.017 kb on - strand at 320.081 kb on + strand at 320.086 kb on - strand at 320.086 kb on - strand at 320.114 kb on - strand at 320.127 kb on - strand at 320.127 kb on - strand at 320.526 kb on + strand, within AZOBR_RS01555 at 320.526 kb on + strand, within AZOBR_RS01555 at 320.527 kb on - strand, within AZOBR_RS01555 at 320.527 kb on - strand, within AZOBR_RS01555 at 320.766 kb on + strand at 320.766 kb on + strand at 320.766 kb on + strand at 320.766 kb on + strand at 320.766 kb on + strand at 320.766 kb on + strand at 320.766 kb on + strand at 320.767 kb on - strand at 320.767 kb on - strand at 320.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 318,275 - AZOBR_RS01545 0.33 -2.9 318,376 + AZOBR_RS01545 0.44 -0.2 318,535 + AZOBR_RS01545 0.62 -2.9 318,535 + AZOBR_RS01545 0.62 -0.0 318,535 + AZOBR_RS01545 0.62 -2.9 318,536 - AZOBR_RS01545 0.62 -3.8 318,579 + AZOBR_RS01545 0.66 +1.3 318,579 + AZOBR_RS01545 0.66 -1.4 318,580 - AZOBR_RS01545 0.66 -0.0 318,580 - AZOBR_RS01545 0.66 -0.1 318,878 - -2.1 318,878 - -3.3 318,924 + -1.2 318,924 + -1.7 318,925 - -1.2 318,925 - -0.9 318,925 - -0.9 318,925 - -1.3 318,925 - -1.0 318,926 + -4.1 318,927 - -0.5 318,931 + -0.3 318,931 + -3.4 318,932 - -0.3 318,932 - +0.2 318,952 + -0.5 318,975 - +0.9 318,975 - +0.4 319,108 + AZOBR_RS01550 0.16 +0.5 319,280 - AZOBR_RS01550 0.33 +1.9 319,471 + AZOBR_RS01550 0.52 -1.4 319,471 + AZOBR_RS01550 0.52 +0.2 319,472 - AZOBR_RS01550 0.52 -1.9 319,483 + AZOBR_RS01550 0.53 -3.8 319,484 - AZOBR_RS01550 0.53 -0.7 319,484 - AZOBR_RS01550 0.53 -0.9 319,484 - AZOBR_RS01550 0.53 -1.2 319,714 + AZOBR_RS01550 0.76 -2.4 319,715 - AZOBR_RS01550 0.76 -0.1 319,715 - AZOBR_RS01550 0.76 -0.5 319,804 + AZOBR_RS01550 0.85 -0.8 319,804 + AZOBR_RS01550 0.85 -1.4 320,015 - +1.8 320,017 - -2.0 320,081 + +0.3 320,086 - -1.0 320,086 - +2.1 320,114 - -1.0 320,127 - -1.1 320,127 - +0.8 320,526 + AZOBR_RS01555 0.58 -0.0 320,526 + AZOBR_RS01555 0.58 +1.5 320,527 - AZOBR_RS01555 0.58 -0.2 320,527 - AZOBR_RS01555 0.58 +0.9 320,766 + +0.9 320,766 + -0.4 320,766 + +0.2 320,766 + -0.0 320,766 + +0.8 320,766 + +0.1 320,766 + -0.6 320,767 - -0.8 320,767 - +0.9 320,889 - +0.4
Or see this region's nucleotide sequence