Experiment: Paraquat dichloride 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01310 and AZOBR_RS01315 are separated by 299 nucleotides AZOBR_RS01315 and AZOBR_RS01320 are separated by 58 nucleotides AZOBR_RS01320 and AZOBR_RS01325 are separated by 22 nucleotides
AZOBR_RS01310: AZOBR_RS01310 - lactate dehydrogenase, at 271,895 to 272,836
_RS01310
AZOBR_RS01315: AZOBR_RS01315 - GCN5 family N-acetyltransferase, at 273,136 to 275,820
_RS01315
AZOBR_RS01320: AZOBR_RS01320 - hypothetical protein, at 275,879 to 276,451
_RS01320
AZOBR_RS01325: AZOBR_RS01325 - 2-hydroxyacid dehydrogenase, at 276,474 to 277,463
_RS01325
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 272.184 kb on + strand, within AZOBR_RS01310 at 272.960 kb on + strand at 272.981 kb on + strand at 273.041 kb on - strand at 273.310 kb on + strand at 273.310 kb on + strand at 273.311 kb on - strand at 273.311 kb on - strand at 273.718 kb on + strand, within AZOBR_RS01315 at 273.719 kb on - strand, within AZOBR_RS01315 at 273.719 kb on - strand at 273.719 kb on - strand, within AZOBR_RS01315 at 273.757 kb on + strand, within AZOBR_RS01315 at 273.758 kb on - strand, within AZOBR_RS01315 at 273.914 kb on - strand, within AZOBR_RS01315 at 273.914 kb on - strand, within AZOBR_RS01315 at 274.420 kb on + strand, within AZOBR_RS01315 at 274.468 kb on + strand, within AZOBR_RS01315 at 274.469 kb on - strand, within AZOBR_RS01315 at 274.597 kb on + strand, within AZOBR_RS01315 at 274.598 kb on - strand, within AZOBR_RS01315 at 274.598 kb on - strand, within AZOBR_RS01315 at 274.894 kb on + strand, within AZOBR_RS01315 at 274.894 kb on + strand, within AZOBR_RS01315 at 274.895 kb on - strand, within AZOBR_RS01315 at 274.987 kb on + strand at 275.471 kb on - strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.534 kb on - strand, within AZOBR_RS01315 at 275.534 kb on - strand at 275.582 kb on - strand at 275.626 kb on + strand at 275.683 kb on + strand at 275.874 kb on + strand at 275.874 kb on + strand at 275.874 kb on + strand at 275.875 kb on - strand at 275.875 kb on - strand at 275.875 kb on - strand at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.434 kb on + strand at 276.434 kb on + strand at 276.435 kb on - strand at 276.450 kb on - strand at 276.475 kb on + strand at 276.476 kb on - strand at 276.476 kb on - strand at 276.799 kb on + strand, within AZOBR_RS01325 at 276.799 kb on + strand, within AZOBR_RS01325
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.04 mg/ml remove 272,184 + AZOBR_RS01310 0.31 +0.2 272,960 + -0.5 272,981 + -3.2 273,041 - -1.2 273,310 + +0.5 273,310 + +3.7 273,311 - -1.1 273,311 - -1.1 273,718 + AZOBR_RS01315 0.22 +0.8 273,719 - AZOBR_RS01315 0.22 -0.8 273,719 - -1.1 273,719 - AZOBR_RS01315 0.22 -3.5 273,757 + AZOBR_RS01315 0.23 -0.2 273,758 - AZOBR_RS01315 0.23 +0.9 273,914 - AZOBR_RS01315 0.29 -2.0 273,914 - AZOBR_RS01315 0.29 +0.2 274,420 + AZOBR_RS01315 0.48 -1.5 274,468 + AZOBR_RS01315 0.50 +0.5 274,469 - AZOBR_RS01315 0.50 -0.1 274,597 + AZOBR_RS01315 0.54 +3.2 274,598 - AZOBR_RS01315 0.54 +1.2 274,598 - AZOBR_RS01315 0.54 -1.1 274,894 + AZOBR_RS01315 0.65 -0.8 274,894 + AZOBR_RS01315 0.65 +0.6 274,895 - AZOBR_RS01315 0.66 -0.9 274,987 + +0.5 275,471 - AZOBR_RS01315 0.87 +1.7 275,533 + AZOBR_RS01315 0.89 +0.2 275,533 + AZOBR_RS01315 0.89 -0.4 275,533 + AZOBR_RS01315 0.89 +0.3 275,534 - AZOBR_RS01315 0.89 -0.5 275,534 - +0.5 275,582 - +1.0 275,626 + -0.4 275,683 + -2.8 275,874 + +0.9 275,874 + +0.1 275,874 + -0.1 275,875 - -1.3 275,875 - -1.8 275,875 - +0.5 276,116 + AZOBR_RS01320 0.41 -0.5 276,116 + AZOBR_RS01320 0.41 -2.0 276,116 + AZOBR_RS01320 0.41 -2.5 276,116 + AZOBR_RS01320 0.41 +0.2 276,434 + +0.6 276,434 + -0.5 276,435 - -0.3 276,450 - +0.8 276,475 + -1.2 276,476 - -1.9 276,476 - +1.2 276,799 + AZOBR_RS01325 0.33 -0.4 276,799 + AZOBR_RS01325 0.33 -2.4
Or see this region's nucleotide sequence