Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4061 and BT4062 are separated by 95 nucleotides BT4062 and BT4063 are separated by 2 nucleotides BT4063 and BT4064 are separated by 10 nucleotides
BT4061: BT4061 - NADH dehydrogenase I, chain K (NCBI ptt file), at 5,296,059 to 5,296,367
BT4061
BT4062: BT4062 - NADH dehydrogenase I, chain J (NCBI ptt file), at 5,296,463 to 5,296,975
BT4062
BT4063: BT4063 - NADH dehydrogenase I, chain I (NCBI ptt file), at 5,296,978 to 5,297,466
BT4063
BT4064: BT4064 - NADH dehydrogenase I, chain H (NCBI ptt file), at 5,297,477 to 5,298,553
BT4064
Position (kb) 5296
5297
5298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5295.987 kb on + strand at 5295.987 kb on + strand at 5295.996 kb on + strand at 5296.012 kb on + strand at 5296.137 kb on + strand, within BT4061 at 5296.137 kb on + strand, within BT4061 at 5296.137 kb on + strand, within BT4061 at 5296.137 kb on + strand, within BT4061 at 5296.257 kb on + strand, within BT4061 at 5296.401 kb on + strand at 5296.442 kb on + strand at 5296.442 kb on + strand at 5296.449 kb on + strand at 5296.452 kb on + strand at 5296.540 kb on + strand, within BT4062 at 5296.542 kb on + strand, within BT4062 at 5296.548 kb on + strand, within BT4062 at 5296.548 kb on + strand, within BT4062 at 5296.592 kb on + strand, within BT4062 at 5296.757 kb on + strand, within BT4062 at 5296.761 kb on + strand, within BT4062 at 5296.761 kb on + strand, within BT4062 at 5296.774 kb on + strand, within BT4062 at 5296.869 kb on + strand, within BT4062 at 5296.928 kb on - strand at 5296.949 kb on + strand at 5296.949 kb on + strand at 5297.127 kb on + strand, within BT4063 at 5297.144 kb on + strand, within BT4063 at 5297.150 kb on + strand, within BT4063 at 5297.234 kb on - strand, within BT4063 at 5297.423 kb on - strand at 5297.438 kb on + strand at 5297.525 kb on + strand at 5297.667 kb on + strand, within BT4064 at 5297.772 kb on + strand, within BT4064 at 5297.784 kb on + strand, within BT4064 at 5297.785 kb on - strand, within BT4064 at 5297.818 kb on + strand, within BT4064 at 5297.901 kb on + strand, within BT4064 at 5297.929 kb on + strand, within BT4064 at 5297.944 kb on + strand, within BT4064 at 5298.043 kb on - strand, within BT4064 at 5298.098 kb on - strand, within BT4064 at 5298.100 kb on + strand, within BT4064 at 5298.121 kb on + strand, within BT4064 at 5298.122 kb on - strand, within BT4064 at 5298.172 kb on + strand, within BT4064 at 5298.196 kb on + strand, within BT4064 at 5298.261 kb on + strand, within BT4064 at 5298.262 kb on - strand, within BT4064 at 5298.318 kb on - strand, within BT4064 at 5298.337 kb on + strand, within BT4064 at 5298.430 kb on + strand, within BT4064 at 5298.435 kb on + strand, within BT4064 at 5298.435 kb on + strand, within BT4064 at 5298.436 kb on - strand, within BT4064 at 5298.436 kb on - strand, within BT4064 at 5298.438 kb on - strand, within BT4064 at 5298.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 5,295,987 + -0.6 5,295,987 + +1.0 5,295,996 + -3.3 5,296,012 + +0.2 5,296,137 + BT4061 0.25 -0.8 5,296,137 + BT4061 0.25 -0.5 5,296,137 + BT4061 0.25 -1.0 5,296,137 + BT4061 0.25 +0.8 5,296,257 + BT4061 0.64 -0.9 5,296,401 + +0.6 5,296,442 + -0.7 5,296,442 + -1.3 5,296,449 + -0.1 5,296,452 + -2.1 5,296,540 + BT4062 0.15 -1.0 5,296,542 + BT4062 0.15 +1.1 5,296,548 + BT4062 0.17 -2.9 5,296,548 + BT4062 0.17 +0.5 5,296,592 + BT4062 0.25 -0.8 5,296,757 + BT4062 0.57 +1.1 5,296,761 + BT4062 0.58 -1.8 5,296,761 + BT4062 0.58 -1.1 5,296,774 + BT4062 0.61 +1.7 5,296,869 + BT4062 0.79 -1.3 5,296,928 - -0.2 5,296,949 + +0.1 5,296,949 + +0.1 5,297,127 + BT4063 0.30 -1.7 5,297,144 + BT4063 0.34 -2.2 5,297,150 + BT4063 0.35 -2.8 5,297,234 - BT4063 0.52 -2.1 5,297,423 - -2.8 5,297,438 + +0.1 5,297,525 + +1.1 5,297,667 + BT4064 0.18 -2.9 5,297,772 + BT4064 0.27 +0.0 5,297,784 + BT4064 0.29 -0.5 5,297,785 - BT4064 0.29 -1.9 5,297,818 + BT4064 0.32 +0.1 5,297,901 + BT4064 0.39 -1.5 5,297,929 + BT4064 0.42 -0.1 5,297,944 + BT4064 0.43 -0.6 5,298,043 - BT4064 0.53 -0.5 5,298,098 - BT4064 0.58 -0.9 5,298,100 + BT4064 0.58 -1.2 5,298,121 + BT4064 0.60 +0.0 5,298,122 - BT4064 0.60 +0.1 5,298,172 + BT4064 0.65 +1.1 5,298,196 + BT4064 0.67 -0.5 5,298,261 + BT4064 0.73 +1.1 5,298,262 - BT4064 0.73 -1.2 5,298,318 - BT4064 0.78 -0.6 5,298,337 + BT4064 0.80 +1.1 5,298,430 + BT4064 0.88 +3.0 5,298,435 + BT4064 0.89 -2.5 5,298,435 + BT4064 0.89 -0.6 5,298,436 - BT4064 0.89 -2.4 5,298,436 - BT4064 0.89 +0.1 5,298,438 - BT4064 0.89 -0.9 5,298,465 - -1.9
Or see this region's nucleotide sequence