Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2061 and BT2062 are separated by 128 nucleotides BT2062 and BT2063 are separated by 131 nucleotides
BT2061: BT2061 - penicillin tolerance protein LytB (NCBI ptt file), at 2,579,881 to 2,580,750
BT2061
BT2062: BT2062 - 6-phosphofructokinase (NCBI ptt file), at 2,580,879 to 2,581,859
BT2062
BT2063: BT2063 - conserved hypothetical protein, putative iron uptake factor (NCBI ptt file), at 2,581,991 to 2,583,280
BT2063
Position (kb)
2580
2581
2582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2579.880 kb on + strand at 2580.741 kb on - strand at 2580.890 kb on + strand at 2580.890 kb on + strand at 2580.972 kb on + strand at 2580.988 kb on + strand, within BT2062 at 2580.989 kb on - strand, within BT2062 at 2580.995 kb on + strand, within BT2062 at 2580.996 kb on - strand, within BT2062 at 2580.996 kb on - strand, within BT2062 at 2580.998 kb on - strand, within BT2062 at 2580.998 kb on - strand, within BT2062 at 2580.999 kb on + strand, within BT2062 at 2580.999 kb on + strand, within BT2062 at 2580.999 kb on + strand, within BT2062 at 2580.999 kb on + strand, within BT2062 at 2580.999 kb on + strand, within BT2062 at 2581.000 kb on - strand, within BT2062 at 2581.000 kb on - strand, within BT2062 at 2581.009 kb on - strand, within BT2062 at 2581.058 kb on - strand, within BT2062 at 2581.061 kb on + strand, within BT2062 at 2581.061 kb on + strand at 2581.149 kb on - strand, within BT2062 at 2581.282 kb on - strand, within BT2062 at 2581.306 kb on - strand, within BT2062 at 2581.750 kb on + strand, within BT2062 at 2581.751 kb on - strand, within BT2062 at 2581.751 kb on - strand, within BT2062 at 2581.776 kb on + strand at 2581.781 kb on - strand at 2581.808 kb on + strand at 2581.838 kb on - strand at 2581.855 kb on + strand at 2581.857 kb on + strand at 2581.858 kb on - strand at 2581.861 kb on - strand at 2581.861 kb on - strand at 2581.861 kb on - strand at 2581.869 kb on + strand at 2581.870 kb on - strand at 2581.884 kb on + strand at 2581.885 kb on - strand at 2581.889 kb on + strand at 2581.949 kb on - strand at 2581.954 kb on + strand at 2581.954 kb on + strand at 2581.954 kb on + strand at 2581.999 kb on + strand at 2582.051 kb on - strand at 2582.076 kb on + strand at 2582.129 kb on + strand, within BT2063 at 2582.130 kb on - strand, within BT2063 at 2582.131 kb on + strand, within BT2063 at 2582.143 kb on + strand, within BT2063 at 2582.144 kb on - strand, within BT2063 at 2582.159 kb on + strand, within BT2063 at 2582.172 kb on + strand, within BT2063 at 2582.173 kb on - strand, within BT2063 at 2582.201 kb on - strand, within BT2063 at 2582.368 kb on - strand, within BT2063 at 2582.382 kb on + strand, within BT2063 at 2582.749 kb on + strand, within BT2063 at 2582.843 kb on - strand, within BT2063 at 2582.858 kb on - strand, within BT2063 at 2582.858 kb on - strand, within BT2063
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 2,579,880 + -0.6 2,580,741 - -1.9 2,580,890 + -1.4 2,580,890 + -0.6 2,580,972 + -0.6 2,580,988 + BT2062 0.11 -0.4 2,580,989 - BT2062 0.11 -1.4 2,580,995 + BT2062 0.12 -1.4 2,580,996 - BT2062 0.12 -2.4 2,580,996 - BT2062 0.12 -0.6 2,580,998 - BT2062 0.12 +0.4 2,580,998 - BT2062 0.12 +0.3 2,580,999 + BT2062 0.12 -1.7 2,580,999 + BT2062 0.12 +0.4 2,580,999 + BT2062 0.12 -0.5 2,580,999 + BT2062 0.12 -0.8 2,580,999 + BT2062 0.12 -2.2 2,581,000 - BT2062 0.12 -0.2 2,581,000 - BT2062 0.12 -1.0 2,581,009 - BT2062 0.13 -1.8 2,581,058 - BT2062 0.18 +0.5 2,581,061 + BT2062 0.19 -0.9 2,581,061 + +1.0 2,581,149 - BT2062 0.28 -0.4 2,581,282 - BT2062 0.41 -0.6 2,581,306 - BT2062 0.44 -0.1 2,581,750 + BT2062 0.89 -1.6 2,581,751 - BT2062 0.89 -1.2 2,581,751 - BT2062 0.89 -0.0 2,581,776 + +2.0 2,581,781 - -0.2 2,581,808 + -1.3 2,581,838 - -1.9 2,581,855 + -0.6 2,581,857 + -0.6 2,581,858 - -1.0 2,581,861 - -0.8 2,581,861 - +1.5 2,581,861 - -0.0 2,581,869 + -0.4 2,581,870 - -3.5 2,581,884 + -0.5 2,581,885 - -1.9 2,581,889 + -1.4 2,581,949 - -0.0 2,581,954 + -1.5 2,581,954 + +1.0 2,581,954 + -0.4 2,581,999 + +1.2 2,582,051 - +1.0 2,582,076 + +0.7 2,582,129 + BT2063 0.11 -3.5 2,582,130 - BT2063 0.11 -2.2 2,582,131 + BT2063 0.11 +0.4 2,582,143 + BT2063 0.12 +0.4 2,582,144 - BT2063 0.12 -1.7 2,582,159 + BT2063 0.13 -1.4 2,582,172 + BT2063 0.14 -0.8 2,582,173 - BT2063 0.14 -0.4 2,582,201 - BT2063 0.16 -0.3 2,582,368 - BT2063 0.29 -1.6 2,582,382 + BT2063 0.30 -1.6 2,582,749 + BT2063 0.59 -2.4 2,582,843 - BT2063 0.66 -2.7 2,582,858 - BT2063 0.67 -2.9 2,582,858 - BT2063 0.67 +1.4
Or see this region's nucleotide sequence