Experiment: BHIS with Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1610 and BT1611 are separated by 128 nucleotides BT1611 and BT1612 overlap by 40 nucleotides BT1612 and BT1613 are separated by 95 nucleotides BT1613 and BT1614 are separated by 47 nucleotides BT1614 and BT1615 are separated by 42 nucleotides
BT1610: BT1610 - DNA polymerase III subunit gamma/tau (NCBI ptt file), at 1,981,122 to 1,982,981
BT1610
BT1611: BT1611 - hypothetical protein (NCBI ptt file), at 1,983,110 to 1,983,478
BT1611
BT1612: BT1612 - hypothetical protein (NCBI ptt file), at 1,983,439 to 1,983,780
BT1612
BT1613: BT1613 - conserved hypothetical protein (NCBI ptt file), at 1,983,876 to 1,984,457
BT1613
BT1614: BT1614 - hypothetical protein (NCBI ptt file), at 1,984,505 to 1,984,738
BT1614
BT1615: BT1615 - aminoacyl-histidine dipeptidase (NCBI ptt file), at 1,984,781 to 1,986,331
BT1615
Position (kb)
1983
1984
1985 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1983.047 kb on - strand at 1983.081 kb on + strand at 1983.081 kb on + strand at 1983.105 kb on + strand at 1983.505 kb on + strand, within BT1612 at 1983.523 kb on + strand, within BT1612 at 1983.535 kb on - strand, within BT1612 at 1983.538 kb on + strand, within BT1612 at 1983.575 kb on + strand, within BT1612 at 1983.575 kb on + strand, within BT1612 at 1983.658 kb on - strand, within BT1612 at 1983.658 kb on - strand, within BT1612 at 1983.722 kb on + strand, within BT1612 at 1983.734 kb on - strand, within BT1612 at 1983.734 kb on - strand at 1983.778 kb on + strand at 1983.779 kb on - strand at 1983.825 kb on + strand at 1983.825 kb on + strand at 1983.877 kb on + strand at 1983.896 kb on - strand at 1983.896 kb on - strand at 1983.915 kb on + strand at 1983.935 kb on + strand, within BT1613 at 1983.935 kb on + strand, within BT1613 at 1983.936 kb on - strand, within BT1613 at 1983.937 kb on + strand, within BT1613 at 1983.937 kb on + strand, within BT1613 at 1983.938 kb on - strand, within BT1613 at 1983.938 kb on - strand, within BT1613 at 1983.945 kb on - strand, within BT1613 at 1983.992 kb on + strand, within BT1613 at 1983.992 kb on + strand, within BT1613 at 1984.008 kb on - strand, within BT1613 at 1984.010 kb on + strand, within BT1613 at 1984.058 kb on + strand, within BT1613 at 1984.059 kb on - strand, within BT1613 at 1984.064 kb on + strand, within BT1613 at 1984.064 kb on + strand, within BT1613 at 1984.065 kb on - strand, within BT1613 at 1984.094 kb on + strand, within BT1613 at 1984.141 kb on + strand, within BT1613 at 1984.141 kb on + strand, within BT1613 at 1984.142 kb on - strand, within BT1613 at 1984.303 kb on + strand, within BT1613 at 1984.304 kb on - strand, within BT1613 at 1984.304 kb on - strand, within BT1613 at 1984.320 kb on - strand, within BT1613 at 1984.320 kb on - strand, within BT1613 at 1984.323 kb on + strand, within BT1613 at 1984.333 kb on + strand, within BT1613 at 1984.334 kb on - strand, within BT1613 at 1984.334 kb on - strand, within BT1613 at 1984.380 kb on - strand, within BT1613 at 1984.395 kb on - strand, within BT1613 at 1984.447 kb on - strand at 1984.463 kb on - strand at 1984.468 kb on - strand at 1984.513 kb on + strand at 1984.513 kb on + strand at 1984.686 kb on - strand, within BT1614 at 1984.686 kb on - strand, within BT1614 at 1984.718 kb on + strand at 1984.846 kb on + strand at 1984.847 kb on - strand at 1984.847 kb on - strand at 1984.975 kb on - strand, within BT1615 at 1985.013 kb on + strand, within BT1615 at 1985.013 kb on + strand, within BT1615 at 1985.083 kb on - strand, within BT1615 at 1985.128 kb on - strand, within BT1615 at 1985.227 kb on - strand, within BT1615
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Vancomycin 0.016 mg/ml remove 1,983,047 - -0.7 1,983,081 + +0.6 1,983,081 + -1.5 1,983,105 + -0.9 1,983,505 + BT1612 0.19 +1.6 1,983,523 + BT1612 0.25 +0.2 1,983,535 - BT1612 0.28 -0.5 1,983,538 + BT1612 0.29 -0.1 1,983,575 + BT1612 0.40 -0.0 1,983,575 + BT1612 0.40 +1.4 1,983,658 - BT1612 0.64 -0.6 1,983,658 - BT1612 0.64 -2.0 1,983,722 + BT1612 0.83 -0.5 1,983,734 - BT1612 0.86 +0.8 1,983,734 - -0.0 1,983,778 + -0.7 1,983,779 - +0.2 1,983,825 + +0.6 1,983,825 + +2.0 1,983,877 + -0.3 1,983,896 - +4.4 1,983,896 - -0.0 1,983,915 + +1.7 1,983,935 + BT1613 0.10 +1.1 1,983,935 + BT1613 0.10 +0.7 1,983,936 - BT1613 0.10 +1.8 1,983,937 + BT1613 0.10 +1.5 1,983,937 + BT1613 0.10 +4.1 1,983,938 - BT1613 0.11 +2.4 1,983,938 - BT1613 0.11 +2.1 1,983,945 - BT1613 0.12 +0.3 1,983,992 + BT1613 0.20 +1.9 1,983,992 + BT1613 0.20 +1.5 1,984,008 - BT1613 0.23 -0.2 1,984,010 + BT1613 0.23 +3.0 1,984,058 + BT1613 0.31 +3.0 1,984,059 - BT1613 0.31 +3.1 1,984,064 + BT1613 0.32 +0.6 1,984,064 + BT1613 0.32 +0.6 1,984,065 - BT1613 0.32 +3.3 1,984,094 + BT1613 0.37 +0.1 1,984,141 + BT1613 0.46 +2.6 1,984,141 + BT1613 0.46 +0.5 1,984,142 - BT1613 0.46 +0.1 1,984,303 + BT1613 0.73 +3.1 1,984,304 - BT1613 0.74 +3.2 1,984,304 - BT1613 0.74 -0.3 1,984,320 - BT1613 0.76 +2.9 1,984,320 - BT1613 0.76 -0.2 1,984,323 + BT1613 0.77 +4.1 1,984,333 + BT1613 0.79 +0.3 1,984,334 - BT1613 0.79 +0.9 1,984,334 - BT1613 0.79 +3.5 1,984,380 - BT1613 0.87 +1.9 1,984,395 - BT1613 0.89 +3.2 1,984,447 - +3.8 1,984,463 - +2.5 1,984,468 - +3.5 1,984,513 + +0.7 1,984,513 + +3.5 1,984,686 - BT1614 0.77 +1.0 1,984,686 - BT1614 0.77 +0.4 1,984,718 + +1.4 1,984,846 + +1.3 1,984,847 - -2.3 1,984,847 - +0.8 1,984,975 - BT1615 0.13 -0.5 1,985,013 + BT1615 0.15 -0.5 1,985,013 + BT1615 0.15 +0.6 1,985,083 - BT1615 0.19 -0.8 1,985,128 - BT1615 0.22 -2.6 1,985,227 - BT1615 0.29 +0.8
Or see this region's nucleotide sequence