Experiment: Paraquat dichloride 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS29555 and AZOBR_RS29560 are separated by 117 nucleotides AZOBR_RS29560 and AZOBR_RS29565 overlap by 4 nucleotides AZOBR_RS29565 and AZOBR_RS29570 are separated by 25 nucleotides
AZOBR_RS29555: AZOBR_RS29555 - enterobactin synthase subunit E, at 755,149 to 756,780
_RS29555
AZOBR_RS29560: AZOBR_RS29560 - isochorismatase, at 756,898 to 757,773
_RS29560
AZOBR_RS29565: AZOBR_RS29565 - 2 3-dihydroxybenzoate-2 3-dehydrogenase, at 757,770 to 758,522
_RS29565
AZOBR_RS29570: AZOBR_RS29570 - antibiotic synthesis protein MbtH, at 758,548 to 758,754
_RS29570
Position (kb) 756
757
758 Strain fitness (log2 ratio) -2
-1
0
1 at 756.166 kb on + strand, within AZOBR_RS29555 at 756.166 kb on + strand, within AZOBR_RS29555 at 756.166 kb on + strand, within AZOBR_RS29555 at 756.311 kb on - strand, within AZOBR_RS29555 at 756.325 kb on + strand, within AZOBR_RS29555 at 756.328 kb on + strand, within AZOBR_RS29555 at 756.329 kb on - strand, within AZOBR_RS29555 at 756.329 kb on - strand, within AZOBR_RS29555 at 756.922 kb on + strand at 756.923 kb on - strand at 756.923 kb on - strand at 757.018 kb on + strand, within AZOBR_RS29560 at 757.018 kb on + strand, within AZOBR_RS29560 at 757.019 kb on - strand, within AZOBR_RS29560 at 757.019 kb on - strand, within AZOBR_RS29560 at 757.033 kb on + strand, within AZOBR_RS29560 at 757.034 kb on - strand, within AZOBR_RS29560 at 757.123 kb on + strand, within AZOBR_RS29560 at 757.351 kb on + strand, within AZOBR_RS29560 at 757.472 kb on - strand, within AZOBR_RS29560 at 758.086 kb on + strand, within AZOBR_RS29565 at 758.520 kb on + strand at 758.563 kb on + strand at 758.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.02 mg/ml remove 756,166 + AZOBR_RS29555 0.62 -0.1 756,166 + AZOBR_RS29555 0.62 +1.6 756,166 + AZOBR_RS29555 0.62 -0.1 756,311 - AZOBR_RS29555 0.71 -0.6 756,325 + AZOBR_RS29555 0.72 -1.0 756,328 + AZOBR_RS29555 0.72 +0.7 756,329 - AZOBR_RS29555 0.72 -2.1 756,329 - AZOBR_RS29555 0.72 -1.5 756,922 + -0.5 756,923 - -2.7 756,923 - +0.4 757,018 + AZOBR_RS29560 0.14 -2.3 757,018 + AZOBR_RS29560 0.14 -0.4 757,019 - AZOBR_RS29560 0.14 +0.0 757,019 - AZOBR_RS29560 0.14 -2.1 757,033 + AZOBR_RS29560 0.15 -0.1 757,034 - AZOBR_RS29560 0.16 +0.8 757,123 + AZOBR_RS29560 0.26 +0.3 757,351 + AZOBR_RS29560 0.52 +0.7 757,472 - AZOBR_RS29560 0.66 -2.7 758,086 + AZOBR_RS29565 0.42 +0.2 758,520 + -1.1 758,563 + +1.4 758,564 - -0.4
Or see this region's nucleotide sequence