Experiment: Paraquat dichloride 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS27625 and AZOBR_RS27630 overlap by 4 nucleotides AZOBR_RS27630 and AZOBR_RS27635 overlap by 1 nucleotides AZOBR_RS27635 and AZOBR_RS27640 are separated by 66 nucleotides
AZOBR_RS27625: AZOBR_RS27625 - hypothetical protein, at 360,515 to 361,201
_RS27625
AZOBR_RS27630: AZOBR_RS27630 - CoA-transferase, at 361,198 to 362,391
_RS27630
AZOBR_RS27635: AZOBR_RS27635 - enoyl-CoA hydratase, at 362,391 to 363,212
_RS27635
AZOBR_RS27640: AZOBR_RS27640 - ABC transporter substrate-binding protein, at 363,279 to 364,250
_RS27640
Position (kb) 361
362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 360.398 kb on + strand at 360.399 kb on - strand at 360.515 kb on - strand at 360.981 kb on - strand, within AZOBR_RS27625 at 361.894 kb on + strand, within AZOBR_RS27630 at 362.152 kb on + strand, within AZOBR_RS27630 at 362.153 kb on - strand, within AZOBR_RS27630 at 362.153 kb on - strand, within AZOBR_RS27630 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.013 kb on - strand, within AZOBR_RS27635 at 363.210 kb on + strand at 363.211 kb on - strand at 363.246 kb on + strand at 363.246 kb on + strand at 363.246 kb on + strand at 363.247 kb on - strand at 363.391 kb on - strand, within AZOBR_RS27640 at 363.391 kb on - strand, within AZOBR_RS27640
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.02 mg/ml remove 360,398 + +1.1 360,399 - -2.8 360,515 - -2.4 360,981 - AZOBR_RS27625 0.68 -0.7 361,894 + AZOBR_RS27630 0.58 -1.0 362,152 + AZOBR_RS27630 0.80 +1.4 362,153 - AZOBR_RS27630 0.80 -0.7 362,153 - AZOBR_RS27630 0.80 -0.6 363,013 - AZOBR_RS27635 0.76 +0.8 363,013 - AZOBR_RS27635 0.76 -1.9 363,013 - AZOBR_RS27635 0.76 -0.1 363,210 + +0.1 363,211 - +1.2 363,246 + -0.6 363,246 + -0.0 363,246 + +0.0 363,247 - +1.6 363,391 - AZOBR_RS27640 0.12 +1.1 363,391 - AZOBR_RS27640 0.12 +1.3
Or see this region's nucleotide sequence