Experiment: Paraquat dichloride 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04065 and AZOBR_RS04070 are separated by 95 nucleotides AZOBR_RS04070 and AZOBR_RS04075 are separated by 117 nucleotides AZOBR_RS04075 and AZOBR_RS04080 are separated by 195 nucleotides
AZOBR_RS04065: AZOBR_RS04065 - ribosome-associated protein IOJAP, at 856,244 to 856,585
_RS04065
AZOBR_RS04070: AZOBR_RS04070 - nicotinic acid mononucleotide adenylyltransferase, at 856,681 to 857,298
_RS04070
AZOBR_RS04075: AZOBR_RS04075 - gamma-glutamyl phosphate reductase, at 857,416 to 858,696
_RS04075
AZOBR_RS04080: AZOBR_RS04080 - cytochrome C, at 858,892 to 859,758
_RS04080
Position (kb)
857
858
859 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 856.664 kb on + strand at 856.664 kb on + strand at 856.664 kb on + strand at 857.314 kb on - strand at 857.314 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.411 kb on - strand at 858.855 kb on - strand at 859.124 kb on - strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.423 kb on + strand, within AZOBR_RS04080 at 859.432 kb on + strand, within AZOBR_RS04080 at 859.433 kb on - strand, within AZOBR_RS04080 at 859.433 kb on - strand, within AZOBR_RS04080 at 859.640 kb on + strand, within AZOBR_RS04080 at 859.640 kb on + strand, within AZOBR_RS04080 at 859.685 kb on - strand at 859.685 kb on - strand at 859.687 kb on + strand at 859.687 kb on + strand at 859.687 kb on + strand at 859.688 kb on - strand at 859.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.02 mg/ml remove 856,664 + -0.2 856,664 + -1.9 856,664 + -2.5 857,314 - +1.7 857,314 - -0.6 857,396 - -2.1 857,396 - -0.3 857,396 - -3.4 857,411 - -1.0 858,855 - +0.8 859,124 - AZOBR_RS04080 0.27 -0.2 859,297 + AZOBR_RS04080 0.47 -3.9 859,297 + AZOBR_RS04080 0.47 -0.6 859,297 + AZOBR_RS04080 0.47 +0.7 859,298 - AZOBR_RS04080 0.47 +0.2 859,298 - AZOBR_RS04080 0.47 +0.0 859,298 - AZOBR_RS04080 0.47 -2.8 859,423 + AZOBR_RS04080 0.61 +0.2 859,432 + AZOBR_RS04080 0.62 +1.7 859,433 - AZOBR_RS04080 0.62 +0.1 859,433 - AZOBR_RS04080 0.62 -1.3 859,640 + AZOBR_RS04080 0.86 +2.8 859,640 + AZOBR_RS04080 0.86 +1.2 859,685 - -1.1 859,685 - +0.9 859,687 + -0.9 859,687 + +0.3 859,687 + -0.0 859,688 - -0.4 859,688 - +0.0
Or see this region's nucleotide sequence