Experiment: Paraquat dichloride 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04060 and AZOBR_RS04065 are separated by 42 nucleotides AZOBR_RS04065 and AZOBR_RS04070 are separated by 95 nucleotides AZOBR_RS04070 and AZOBR_RS04075 are separated by 117 nucleotides
AZOBR_RS04060: AZOBR_RS04060 - 50S rRNA methyltransferase, at 855,743 to 856,201
_RS04060
AZOBR_RS04065: AZOBR_RS04065 - ribosome-associated protein IOJAP, at 856,244 to 856,585
_RS04065
AZOBR_RS04070: AZOBR_RS04070 - nicotinic acid mononucleotide adenylyltransferase, at 856,681 to 857,298
_RS04070
AZOBR_RS04075: AZOBR_RS04075 - gamma-glutamyl phosphate reductase, at 857,416 to 858,696
_RS04075
Position (kb) 856
857
858 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 855.823 kb on + strand, within AZOBR_RS04060 at 855.824 kb on - strand, within AZOBR_RS04060 at 856.135 kb on - strand, within AZOBR_RS04060 at 856.144 kb on - strand, within AZOBR_RS04060 at 856.308 kb on + strand, within AZOBR_RS04065 at 856.309 kb on - strand, within AZOBR_RS04065 at 856.664 kb on + strand at 856.664 kb on + strand at 856.664 kb on + strand at 857.314 kb on - strand at 857.314 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.02 mg/ml remove 855,823 + AZOBR_RS04060 0.17 +0.6 855,824 - AZOBR_RS04060 0.18 -0.1 856,135 - AZOBR_RS04060 0.85 +0.8 856,144 - AZOBR_RS04060 0.87 -0.1 856,308 + AZOBR_RS04065 0.19 +0.3 856,309 - AZOBR_RS04065 0.19 +0.0 856,664 + -0.2 856,664 + -1.9 856,664 + -2.5 857,314 - +1.7 857,314 - -0.6 857,396 - -2.1 857,396 - -0.3 857,396 - -3.4 857,411 - -1.0
Or see this region's nucleotide sequence