Experiment: Paraquat dichloride 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00645 and AZOBR_RS00650 overlap by 4 nucleotides AZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotides AZOBR_RS00655 and AZOBR_RS00660 overlap by 1 nucleotides AZOBR_RS00660 and AZOBR_RS00665 are separated by 206 nucleotides
AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576
_RS00645
AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481
_RS00650
AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833
_RS00655
AZOBR_RS00660: AZOBR_RS00660 - hypothetical protein, at 131,833 to 132,117
_RS00660
AZOBR_RS00665: AZOBR_RS00665 - tRNA-Ala, at 132,324 to 132,399
_RS00665
Position (kb)
130
131
132 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 129.588 kb on + strand at 129.588 kb on + strand at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.378 kb on - strand, within AZOBR_RS00645 at 131.849 kb on + strand at 132.075 kb on - strand, within AZOBR_RS00660 at 132.366 kb on + strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.02 mg/ml remove 129,588 + -0.4 129,588 + +0.4 130,356 + AZOBR_RS00645 0.78 -0.8 130,356 + AZOBR_RS00645 0.78 -0.8 130,356 + AZOBR_RS00645 0.78 -0.5 130,357 - AZOBR_RS00645 0.78 -2.7 130,357 - AZOBR_RS00645 0.78 +0.1 130,378 - AZOBR_RS00645 0.80 -2.6 131,849 + +0.2 132,075 - AZOBR_RS00660 0.85 +0.8 132,366 + AZOBR_RS00665 0.55 +1.8 132,367 - AZOBR_RS00665 0.57 -2.5 132,367 - AZOBR_RS00665 0.57 -0.7
Or see this region's nucleotide sequence