Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_29335 and AO356_29340 are separated by 34 nucleotides AO356_29340 and AO356_29345 are separated by 68 nucleotides AO356_29345 and AO356_29350 are separated by 370 nucleotides AO356_29350 and AO356_29355 are separated by 171 nucleotides
AO356_29335: AO356_29335 - sugar dehydrogenase, at 6,823,342 to 6,824,103
_29335
AO356_29340: AO356_29340 - multidrug DMT transporter permease, at 6,824,138 to 6,824,965
_29340
AO356_29345: AO356_29345 - LysR family transcriptional regulator, at 6,825,034 to 6,825,939
_29345
AO356_29350: AO356_29350 - hypothetical protein, at 6,826,310 to 6,826,585
_29350
AO356_29355: AO356_29355 - hypothetical protein, at 6,826,757 to 6,827,197
_29355
Position (kb)
6825
6826 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6824.299 kb on - strand, within AO356_29340 at 6824.299 kb on - strand, within AO356_29340 at 6824.299 kb on - strand, within AO356_29340 at 6824.313 kb on + strand, within AO356_29340 at 6824.313 kb on + strand, within AO356_29340 at 6824.357 kb on - strand, within AO356_29340 at 6824.403 kb on + strand, within AO356_29340 at 6824.403 kb on + strand, within AO356_29340 at 6824.404 kb on - strand, within AO356_29340 at 6824.452 kb on - strand, within AO356_29340 at 6824.484 kb on - strand, within AO356_29340 at 6824.484 kb on - strand, within AO356_29340 at 6824.484 kb on - strand, within AO356_29340 at 6824.484 kb on - strand, within AO356_29340 at 6824.536 kb on - strand, within AO356_29340 at 6824.570 kb on - strand, within AO356_29340 at 6824.727 kb on + strand, within AO356_29340 at 6824.728 kb on - strand, within AO356_29340 at 6824.958 kb on - strand at 6824.976 kb on - strand at 6824.976 kb on - strand at 6825.097 kb on - strand at 6825.127 kb on + strand, within AO356_29345 at 6825.127 kb on + strand, within AO356_29345 at 6825.127 kb on + strand, within AO356_29345 at 6825.128 kb on - strand, within AO356_29345 at 6825.128 kb on - strand, within AO356_29345 at 6825.134 kb on + strand, within AO356_29345 at 6825.134 kb on + strand, within AO356_29345 at 6825.134 kb on + strand, within AO356_29345 at 6825.135 kb on - strand, within AO356_29345 at 6825.137 kb on + strand, within AO356_29345 at 6825.138 kb on - strand, within AO356_29345 at 6825.138 kb on - strand, within AO356_29345 at 6825.138 kb on - strand, within AO356_29345 at 6825.138 kb on - strand, within AO356_29345 at 6825.138 kb on - strand, within AO356_29345 at 6825.264 kb on + strand, within AO356_29345 at 6825.264 kb on + strand, within AO356_29345 at 6825.265 kb on - strand, within AO356_29345 at 6825.265 kb on - strand, within AO356_29345 at 6825.271 kb on - strand, within AO356_29345 at 6825.271 kb on - strand, within AO356_29345 at 6825.370 kb on - strand, within AO356_29345 at 6825.412 kb on - strand, within AO356_29345 at 6825.641 kb on + strand, within AO356_29345 at 6825.952 kb on + strand at 6825.994 kb on + strand at 6826.040 kb on + strand at 6826.040 kb on + strand at 6826.040 kb on + strand at 6826.041 kb on - strand at 6826.041 kb on - strand at 6826.302 kb on + strand at 6826.358 kb on + strand, within AO356_29350 at 6826.359 kb on - strand, within AO356_29350 at 6826.423 kb on + strand, within AO356_29350 at 6826.424 kb on - strand, within AO356_29350 at 6826.511 kb on - strand, within AO356_29350 at 6826.583 kb on + strand at 6826.584 kb on - strand at 6826.584 kb on - strand at 6826.584 kb on - strand at 6826.589 kb on + strand at 6826.590 kb on - strand at 6826.590 kb on - strand at 6826.712 kb on + strand at 6826.774 kb on + strand at 6826.774 kb on + strand at 6826.774 kb on + strand at 6826.775 kb on - strand at 6826.775 kb on - strand at 6826.775 kb on - strand at 6826.775 kb on - strand at 6826.775 kb on - strand at 6826.775 kb on - strand at 6826.778 kb on + strand at 6826.778 kb on + strand at 6826.779 kb on - strand at 6826.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 6,824,299 - AO356_29340 0.19 -0.9 6,824,299 - AO356_29340 0.19 +0.6 6,824,299 - AO356_29340 0.19 +0.4 6,824,313 + AO356_29340 0.21 -0.8 6,824,313 + AO356_29340 0.21 +1.0 6,824,357 - AO356_29340 0.26 +0.7 6,824,403 + AO356_29340 0.32 +0.4 6,824,403 + AO356_29340 0.32 -0.5 6,824,404 - AO356_29340 0.32 +0.7 6,824,452 - AO356_29340 0.38 +0.0 6,824,484 - AO356_29340 0.42 -0.2 6,824,484 - AO356_29340 0.42 +0.8 6,824,484 - AO356_29340 0.42 +0.8 6,824,484 - AO356_29340 0.42 -0.1 6,824,536 - AO356_29340 0.48 +0.0 6,824,570 - AO356_29340 0.52 +1.1 6,824,727 + AO356_29340 0.71 -0.3 6,824,728 - AO356_29340 0.71 +1.2 6,824,958 - -0.5 6,824,976 - +0.9 6,824,976 - +0.5 6,825,097 - +2.0 6,825,127 + AO356_29345 0.10 -0.9 6,825,127 + AO356_29345 0.10 -0.1 6,825,127 + AO356_29345 0.10 -0.3 6,825,128 - AO356_29345 0.10 +0.4 6,825,128 - AO356_29345 0.10 -0.5 6,825,134 + AO356_29345 0.11 -0.0 6,825,134 + AO356_29345 0.11 +0.3 6,825,134 + AO356_29345 0.11 -1.0 6,825,135 - AO356_29345 0.11 +0.5 6,825,137 + AO356_29345 0.11 -1.3 6,825,138 - AO356_29345 0.11 +1.3 6,825,138 - AO356_29345 0.11 +0.8 6,825,138 - AO356_29345 0.11 +0.6 6,825,138 - AO356_29345 0.11 -0.5 6,825,138 - AO356_29345 0.11 +0.8 6,825,264 + AO356_29345 0.25 +3.3 6,825,264 + AO356_29345 0.25 -0.3 6,825,265 - AO356_29345 0.25 -0.9 6,825,265 - AO356_29345 0.25 -0.1 6,825,271 - AO356_29345 0.26 -0.4 6,825,271 - AO356_29345 0.26 -2.5 6,825,370 - AO356_29345 0.37 -0.1 6,825,412 - AO356_29345 0.42 +0.6 6,825,641 + AO356_29345 0.67 +0.6 6,825,952 + -1.6 6,825,994 + -0.0 6,826,040 + +0.4 6,826,040 + -1.5 6,826,040 + -0.8 6,826,041 - +0.9 6,826,041 - +0.6 6,826,302 + +0.4 6,826,358 + AO356_29350 0.17 -1.4 6,826,359 - AO356_29350 0.18 -0.2 6,826,423 + AO356_29350 0.41 -0.2 6,826,424 - AO356_29350 0.41 -0.5 6,826,511 - AO356_29350 0.73 +0.0 6,826,583 + +0.4 6,826,584 - -0.3 6,826,584 - -2.3 6,826,584 - +0.9 6,826,589 + -0.3 6,826,590 - +0.1 6,826,590 - -0.3 6,826,712 + +0.5 6,826,774 + -0.1 6,826,774 + -0.6 6,826,774 + -1.3 6,826,775 - -0.8 6,826,775 - -0.3 6,826,775 - -0.8 6,826,775 - +0.6 6,826,775 - -0.1 6,826,775 - -2.5 6,826,778 + -0.4 6,826,778 + -0.2 6,826,779 - -0.2 6,826,779 - -0.8
Or see this region's nucleotide sequence