Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24600 and AO356_24605 are separated by 237 nucleotides AO356_24605 and AO356_24610 overlap by 14 nucleotides AO356_24610 and AO356_24615 are separated by 156 nucleotides AO356_24615 and AO356_24620 are separated by 449 nucleotides
AO356_24600: AO356_24600 - ketoglutarate semialdehyde dehydrogenase, at 5,562,432 to 5,564,012
_24600
AO356_24605: AO356_24605 - hypothetical protein, at 5,564,250 to 5,564,546
_24605
AO356_24610: AO356_24610 - 3-carboxymuconate cyclase, at 5,564,533 to 5,565,708
_24610
AO356_24615: AO356_24615 - hypothetical protein, at 5,565,865 to 5,566,044
_24615
AO356_24620: AO356_24620 - glutathione S-transferase, at 5,566,494 to 5,567,096
_24620
Position (kb) 5564
5565
5566 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5563.917 kb on + strand at 5563.918 kb on - strand at 5564.109 kb on + strand at 5564.109 kb on + strand at 5564.109 kb on + strand at 5564.109 kb on + strand at 5564.110 kb on - strand at 5564.110 kb on - strand at 5564.125 kb on - strand at 5564.125 kb on - strand at 5564.214 kb on + strand at 5564.635 kb on + strand at 5564.635 kb on + strand at 5564.635 kb on + strand at 5564.636 kb on - strand at 5564.636 kb on - strand at 5564.636 kb on - strand at 5564.663 kb on - strand, within AO356_24610 at 5564.830 kb on + strand, within AO356_24610 at 5565.115 kb on + strand, within AO356_24610 at 5565.115 kb on + strand, within AO356_24610 at 5565.115 kb on + strand, within AO356_24610 at 5565.115 kb on + strand, within AO356_24610 at 5565.116 kb on - strand, within AO356_24610 at 5565.116 kb on - strand, within AO356_24610 at 5565.116 kb on - strand, within AO356_24610 at 5565.116 kb on - strand, within AO356_24610 at 5565.116 kb on - strand, within AO356_24610 at 5565.157 kb on + strand, within AO356_24610 at 5565.158 kb on - strand, within AO356_24610 at 5565.164 kb on - strand, within AO356_24610 at 5565.433 kb on + strand, within AO356_24610 at 5565.707 kb on - strand at 5565.745 kb on - strand at 5565.760 kb on - strand at 5565.760 kb on - strand at 5565.770 kb on - strand at 5565.770 kb on - strand at 5565.770 kb on - strand at 5565.770 kb on - strand at 5565.976 kb on + strand, within AO356_24615 at 5565.977 kb on - strand, within AO356_24615 at 5565.988 kb on + strand, within AO356_24615 at 5565.988 kb on + strand, within AO356_24615 at 5565.989 kb on - strand, within AO356_24615 at 5566.012 kb on + strand, within AO356_24615 at 5566.012 kb on + strand, within AO356_24615 at 5566.206 kb on + strand at 5566.206 kb on + strand at 5566.206 kb on + strand at 5566.207 kb on - strand at 5566.207 kb on - strand at 5566.207 kb on - strand at 5566.256 kb on + strand at 5566.324 kb on - strand at 5566.367 kb on + strand at 5566.368 kb on - strand at 5566.495 kb on + strand at 5566.612 kb on - strand, within AO356_24620
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,563,917 + -0.5 5,563,918 - -0.5 5,564,109 + -0.0 5,564,109 + -1.0 5,564,109 + +0.4 5,564,109 + +1.6 5,564,110 - -0.5 5,564,110 - +0.2 5,564,125 - +0.9 5,564,125 - +0.7 5,564,214 + +0.2 5,564,635 + +1.2 5,564,635 + +1.2 5,564,635 + -1.3 5,564,636 - +0.3 5,564,636 - -0.8 5,564,636 - +0.6 5,564,663 - AO356_24610 0.11 -0.3 5,564,830 + AO356_24610 0.25 +0.6 5,565,115 + AO356_24610 0.49 +0.6 5,565,115 + AO356_24610 0.49 +0.1 5,565,115 + AO356_24610 0.49 +0.0 5,565,115 + AO356_24610 0.49 -1.7 5,565,116 - AO356_24610 0.50 +1.0 5,565,116 - AO356_24610 0.50 -2.7 5,565,116 - AO356_24610 0.50 -0.5 5,565,116 - AO356_24610 0.50 -0.2 5,565,116 - AO356_24610 0.50 +1.1 5,565,157 + AO356_24610 0.53 +0.7 5,565,158 - AO356_24610 0.53 -0.3 5,565,164 - AO356_24610 0.54 +0.7 5,565,433 + AO356_24610 0.77 +0.2 5,565,707 - +1.4 5,565,745 - -1.1 5,565,760 - -0.5 5,565,760 - +0.5 5,565,770 - -0.6 5,565,770 - +1.4 5,565,770 - +0.6 5,565,770 - +0.5 5,565,976 + AO356_24615 0.62 -2.0 5,565,977 - AO356_24615 0.62 -0.8 5,565,988 + AO356_24615 0.68 -0.8 5,565,988 + AO356_24615 0.68 +2.1 5,565,989 - AO356_24615 0.69 -1.1 5,566,012 + AO356_24615 0.82 +0.2 5,566,012 + AO356_24615 0.82 -2.4 5,566,206 + +1.8 5,566,206 + -1.9 5,566,206 + +0.6 5,566,207 - -0.2 5,566,207 - -2.0 5,566,207 - -0.1 5,566,256 + +1.2 5,566,324 - -0.9 5,566,367 + +0.5 5,566,368 - -0.1 5,566,495 + -0.0 5,566,612 - AO356_24620 0.20 +1.4
Or see this region's nucleotide sequence