Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_24240 and AO356_24245 are separated by 235 nucleotides AO356_24245 and AO356_24250 are separated by 42 nucleotides AO356_24250 and AO356_24255 are separated by 2 nucleotides
AO356_24240: AO356_24240 - ABC transporter ATP-binding protein, at 5,438,602 to 5,440,305
_24240
AO356_24245: AO356_24245 - siderophore-iron reductase FhuF, at 5,440,541 to 5,441,194
_24245
AO356_24250: AO356_24250 - ABC transporter ATP-binding protein, at 5,441,237 to 5,442,883
_24250
AO356_24255: AO356_24255 - non-ribosomal peptide synthetase, at 5,442,886 to 5,446,737
_24255
Position (kb)
5441
5442
5443 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5440.389 kb on + strand at 5440.459 kb on - strand at 5440.614 kb on - strand, within AO356_24245 at 5440.614 kb on - strand, within AO356_24245 at 5440.778 kb on + strand, within AO356_24245 at 5440.779 kb on - strand, within AO356_24245 at 5441.032 kb on - strand, within AO356_24245 at 5441.073 kb on - strand, within AO356_24245 at 5441.165 kb on + strand at 5441.174 kb on + strand at 5441.174 kb on + strand at 5441.174 kb on + strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.175 kb on - strand at 5441.283 kb on - strand at 5441.283 kb on - strand at 5441.337 kb on + strand at 5441.406 kb on + strand, within AO356_24250 at 5441.406 kb on + strand, within AO356_24250 at 5441.563 kb on + strand, within AO356_24250 at 5441.564 kb on - strand, within AO356_24250 at 5441.814 kb on - strand, within AO356_24250 at 5441.887 kb on - strand, within AO356_24250 at 5441.890 kb on + strand, within AO356_24250 at 5441.891 kb on - strand, within AO356_24250 at 5442.080 kb on - strand, within AO356_24250 at 5442.082 kb on - strand, within AO356_24250 at 5442.153 kb on + strand, within AO356_24250 at 5442.153 kb on + strand, within AO356_24250 at 5442.153 kb on + strand, within AO356_24250 at 5442.153 kb on + strand, within AO356_24250 at 5442.154 kb on - strand, within AO356_24250 at 5442.154 kb on - strand, within AO356_24250 at 5442.189 kb on + strand, within AO356_24250 at 5442.190 kb on - strand, within AO356_24250 at 5442.463 kb on - strand, within AO356_24250 at 5442.746 kb on + strand at 5442.747 kb on - strand at 5442.961 kb on - strand at 5442.961 kb on - strand at 5443.125 kb on + strand at 5443.125 kb on + strand at 5443.125 kb on + strand at 5443.126 kb on - strand at 5443.126 kb on - strand at 5443.523 kb on + strand, within AO356_24255 at 5443.523 kb on + strand, within AO356_24255 at 5443.524 kb on - strand, within AO356_24255
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,440,389 + -0.7 5,440,459 - +1.0 5,440,614 - AO356_24245 0.11 +0.1 5,440,614 - AO356_24245 0.11 +0.4 5,440,778 + AO356_24245 0.36 +0.1 5,440,779 - AO356_24245 0.36 -1.4 5,441,032 - AO356_24245 0.75 -0.8 5,441,073 - AO356_24245 0.81 -1.4 5,441,165 + +0.6 5,441,174 + -1.2 5,441,174 + -1.6 5,441,174 + -2.3 5,441,175 - +1.6 5,441,175 - +1.7 5,441,175 - -0.1 5,441,175 - -0.4 5,441,175 - -0.1 5,441,175 - -0.6 5,441,175 - +2.3 5,441,175 - -0.1 5,441,175 - +0.2 5,441,283 - +0.4 5,441,283 - +1.9 5,441,337 + +0.6 5,441,406 + AO356_24250 0.10 -0.8 5,441,406 + AO356_24250 0.10 +1.2 5,441,563 + AO356_24250 0.20 -0.7 5,441,564 - AO356_24250 0.20 -0.6 5,441,814 - AO356_24250 0.35 -0.2 5,441,887 - AO356_24250 0.39 -0.4 5,441,890 + AO356_24250 0.40 +2.4 5,441,891 - AO356_24250 0.40 +0.7 5,442,080 - AO356_24250 0.51 +0.5 5,442,082 - AO356_24250 0.51 -2.8 5,442,153 + AO356_24250 0.56 +0.5 5,442,153 + AO356_24250 0.56 +0.4 5,442,153 + AO356_24250 0.56 -0.3 5,442,153 + AO356_24250 0.56 -2.0 5,442,154 - AO356_24250 0.56 +0.6 5,442,154 - AO356_24250 0.56 +1.6 5,442,189 + AO356_24250 0.58 -0.2 5,442,190 - AO356_24250 0.58 +0.9 5,442,463 - AO356_24250 0.74 -1.8 5,442,746 + +2.7 5,442,747 - -0.1 5,442,961 - -0.7 5,442,961 - +1.1 5,443,125 + +1.2 5,443,125 + -1.6 5,443,125 + -0.3 5,443,126 - -0.3 5,443,126 - +0.4 5,443,523 + AO356_24255 0.17 +0.1 5,443,523 + AO356_24255 0.17 -1.2 5,443,524 - AO356_24255 0.17 -0.6
Or see this region's nucleotide sequence