Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21255 and AO356_21260 are separated by 180 nucleotides AO356_21260 and AO356_21265 are separated by 83 nucleotides AO356_21265 and AO356_21270 are separated by 232 nucleotides AO356_21270 and AO356_21275 overlap by 1 nucleotides AO356_21275 and AO356_21280 overlap by 1 nucleotides
AO356_21255: AO356_21255 - alpha/beta hydrolase, at 4,770,537 to 4,771,367
_21255
AO356_21260: AO356_21260 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 4,771,548 to 4,771,904
_21260
AO356_21265: AO356_21265 - hypothetical protein, at 4,771,988 to 4,773,067
_21265
AO356_21270: AO356_21270 - peptidase, at 4,773,300 to 4,773,608
_21270
AO356_21275: AO356_21275 - peptidase, at 4,773,608 to 4,773,919
_21275
AO356_21280: AO356_21280 - transcriptional regulator, at 4,773,919 to 4,774,587
_21280
Position (kb)
4771
4772
4773
4774 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4771.201 kb on + strand, within AO356_21255 at 4771.202 kb on - strand, within AO356_21255 at 4771.202 kb on - strand, within AO356_21255 at 4771.202 kb on - strand, within AO356_21255 at 4771.252 kb on + strand, within AO356_21255 at 4771.253 kb on - strand, within AO356_21255 at 4771.253 kb on - strand, within AO356_21255 at 4771.253 kb on - strand, within AO356_21255 at 4771.339 kb on + strand at 4771.340 kb on - strand at 4771.340 kb on - strand at 4771.374 kb on + strand at 4771.407 kb on + strand at 4771.408 kb on - strand at 4771.408 kb on - strand at 4771.497 kb on + strand at 4771.497 kb on + strand at 4771.498 kb on - strand at 4771.498 kb on - strand at 4771.562 kb on - strand at 4771.562 kb on - strand at 4771.697 kb on - strand, within AO356_21260 at 4771.697 kb on - strand, within AO356_21260 at 4771.919 kb on + strand at 4771.919 kb on + strand at 4771.920 kb on - strand at 4771.920 kb on - strand at 4771.920 kb on - strand at 4771.990 kb on - strand at 4772.170 kb on - strand, within AO356_21265 at 4772.170 kb on - strand, within AO356_21265 at 4772.308 kb on - strand, within AO356_21265 at 4772.326 kb on - strand, within AO356_21265 at 4772.349 kb on + strand, within AO356_21265 at 4772.776 kb on - strand, within AO356_21265 at 4772.776 kb on - strand, within AO356_21265 at 4772.776 kb on - strand, within AO356_21265 at 4772.776 kb on - strand, within AO356_21265 at 4772.776 kb on - strand, within AO356_21265 at 4773.084 kb on + strand at 4773.098 kb on + strand at 4773.098 kb on + strand at 4773.098 kb on + strand at 4773.099 kb on - strand at 4773.100 kb on + strand at 4773.100 kb on + strand at 4773.101 kb on - strand at 4773.208 kb on - strand at 4773.260 kb on - strand at 4773.281 kb on + strand at 4773.282 kb on - strand at 4773.283 kb on + strand at 4773.283 kb on + strand at 4773.284 kb on - strand at 4773.284 kb on - strand at 4773.343 kb on + strand, within AO356_21270 at 4773.344 kb on - strand, within AO356_21270 at 4773.344 kb on - strand, within AO356_21270 at 4773.344 kb on - strand, within AO356_21270 at 4773.344 kb on - strand, within AO356_21270 at 4773.347 kb on + strand, within AO356_21270 at 4773.348 kb on - strand, within AO356_21270 at 4773.507 kb on + strand, within AO356_21270 at 4773.507 kb on + strand, within AO356_21270 at 4773.507 kb on + strand, within AO356_21270 at 4773.508 kb on - strand, within AO356_21270 at 4773.508 kb on - strand, within AO356_21270 at 4773.508 kb on - strand, within AO356_21270 at 4773.513 kb on + strand, within AO356_21270 at 4773.513 kb on + strand, within AO356_21270 at 4773.513 kb on + strand, within AO356_21270 at 4773.514 kb on - strand, within AO356_21270 at 4773.603 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.607 kb on - strand at 4773.824 kb on - strand, within AO356_21275 at 4773.824 kb on - strand, within AO356_21275 at 4773.824 kb on - strand, within AO356_21275 at 4773.908 kb on - strand at 4773.908 kb on - strand at 4774.030 kb on + strand, within AO356_21280 at 4774.031 kb on - strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280 at 4774.051 kb on + strand, within AO356_21280
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 4,771,201 + AO356_21255 0.80 +0.6 4,771,202 - AO356_21255 0.80 +0.2 4,771,202 - AO356_21255 0.80 +1.9 4,771,202 - AO356_21255 0.80 -1.0 4,771,252 + AO356_21255 0.86 -1.4 4,771,253 - AO356_21255 0.86 +0.1 4,771,253 - AO356_21255 0.86 -0.0 4,771,253 - AO356_21255 0.86 -0.7 4,771,339 + -0.5 4,771,340 - +1.0 4,771,340 - -1.1 4,771,374 + -0.8 4,771,407 + -0.4 4,771,408 - -0.8 4,771,408 - -0.7 4,771,497 + -1.5 4,771,497 + +0.1 4,771,498 - +0.5 4,771,498 - +0.6 4,771,562 - -0.6 4,771,562 - -0.1 4,771,697 - AO356_21260 0.42 -0.3 4,771,697 - AO356_21260 0.42 -2.0 4,771,919 + +1.1 4,771,919 + -1.2 4,771,920 - +0.5 4,771,920 - -0.1 4,771,920 - -0.5 4,771,990 - +2.1 4,772,170 - AO356_21265 0.17 +0.5 4,772,170 - AO356_21265 0.17 +0.2 4,772,308 - AO356_21265 0.30 -0.8 4,772,326 - AO356_21265 0.31 +0.7 4,772,349 + AO356_21265 0.33 +1.2 4,772,776 - AO356_21265 0.73 -0.2 4,772,776 - AO356_21265 0.73 -0.3 4,772,776 - AO356_21265 0.73 +0.2 4,772,776 - AO356_21265 0.73 -0.2 4,772,776 - AO356_21265 0.73 -0.3 4,773,084 + -0.0 4,773,098 + -0.3 4,773,098 + -0.9 4,773,098 + +1.2 4,773,099 - -0.3 4,773,100 + +1.7 4,773,100 + +0.2 4,773,101 - -0.3 4,773,208 - +0.7 4,773,260 - +0.1 4,773,281 + -0.7 4,773,282 - -0.7 4,773,283 + +1.1 4,773,283 + +0.1 4,773,284 - +0.5 4,773,284 - +1.1 4,773,343 + AO356_21270 0.14 -0.6 4,773,344 - AO356_21270 0.14 +0.2 4,773,344 - AO356_21270 0.14 +1.0 4,773,344 - AO356_21270 0.14 -0.8 4,773,344 - AO356_21270 0.14 +0.7 4,773,347 + AO356_21270 0.15 +1.6 4,773,348 - AO356_21270 0.16 -0.4 4,773,507 + AO356_21270 0.67 -1.0 4,773,507 + AO356_21270 0.67 -0.0 4,773,507 + AO356_21270 0.67 +0.6 4,773,508 - AO356_21270 0.67 +0.2 4,773,508 - AO356_21270 0.67 +0.2 4,773,508 - AO356_21270 0.67 -0.1 4,773,513 + AO356_21270 0.69 -1.2 4,773,513 + AO356_21270 0.69 +0.6 4,773,513 + AO356_21270 0.69 +0.9 4,773,514 - AO356_21270 0.69 +0.8 4,773,603 - +0.1 4,773,607 - +0.4 4,773,607 - +1.7 4,773,607 - +1.8 4,773,607 - +1.1 4,773,824 - AO356_21275 0.69 +2.5 4,773,824 - AO356_21275 0.69 +1.1 4,773,824 - AO356_21275 0.69 +1.7 4,773,908 - +0.7 4,773,908 - +0.1 4,774,030 + AO356_21280 0.17 -0.4 4,774,031 - AO356_21280 0.17 +0.6 4,774,051 + AO356_21280 0.20 -0.9 4,774,051 + AO356_21280 0.20 -1.7 4,774,051 + AO356_21280 0.20 +0.1
Or see this region's nucleotide sequence