Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_16625 and AO356_16630 are separated by 139 nucleotides AO356_16630 and AO356_16635 are separated by 10 nucleotides AO356_16635 and AO356_16640 are separated by 39 nucleotides AO356_16640 and AO356_16645 are separated by 130 nucleotides
AO356_16625: AO356_16625 - glycerol acyltransferase, at 3,750,924 to 3,752,090
_16625
AO356_16630: AO356_16630 - hypothetical protein, at 3,752,230 to 3,752,412
_16630
AO356_16635: AO356_16635 - hypothetical protein, at 3,752,423 to 3,752,683
_16635
AO356_16640: AO356_16640 - hypothetical protein, at 3,752,723 to 3,753,346
_16640
AO356_16645: AO356_16645 - nickel ABC transporter substrate-binding protein, at 3,753,477 to 3,754,196
_16645
Position (kb)
3752
3753 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.117 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.121 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.163 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.190 kb on - strand at 3752.202 kb on - strand at 3752.202 kb on - strand at 3752.202 kb on - strand at 3752.204 kb on - strand at 3752.204 kb on - strand at 3752.204 kb on - strand at 3752.248 kb on + strand at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.249 kb on - strand, within AO356_16630 at 3752.260 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.314 kb on + strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.315 kb on - strand, within AO356_16630 at 3752.436 kb on - strand at 3752.436 kb on - strand at 3752.594 kb on + strand, within AO356_16635 at 3752.595 kb on - strand, within AO356_16635 at 3752.595 kb on - strand, within AO356_16635 at 3752.618 kb on + strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.619 kb on - strand, within AO356_16635 at 3752.660 kb on + strand at 3752.681 kb on + strand at 3752.681 kb on + strand at 3752.735 kb on + strand at 3752.736 kb on - strand at 3752.809 kb on + strand, within AO356_16640 at 3753.295 kb on + strand at 3753.296 kb on - strand at 3753.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,752,117 - -2.5 3,752,117 - +0.3 3,752,117 - +0.8 3,752,117 - +0.5 3,752,121 - -2.0 3,752,121 - +0.1 3,752,121 - +0.4 3,752,121 - -2.9 3,752,121 - +0.9 3,752,121 - +0.1 3,752,163 - -0.6 3,752,163 - -0.2 3,752,163 - +0.5 3,752,163 - -1.2 3,752,163 - -0.3 3,752,190 - +1.9 3,752,190 - +0.5 3,752,190 - -0.3 3,752,190 - -0.1 3,752,190 - +0.5 3,752,202 - +0.3 3,752,202 - -0.8 3,752,202 - -0.4 3,752,204 - -1.2 3,752,204 - -0.9 3,752,204 - -0.2 3,752,248 + -1.9 3,752,249 - AO356_16630 0.10 +0.4 3,752,249 - AO356_16630 0.10 -1.2 3,752,249 - AO356_16630 0.10 +0.5 3,752,249 - AO356_16630 0.10 -0.9 3,752,249 - AO356_16630 0.10 +0.8 3,752,249 - AO356_16630 0.10 -1.4 3,752,260 + AO356_16630 0.16 -0.8 3,752,314 + AO356_16630 0.46 -0.2 3,752,314 + AO356_16630 0.46 -1.0 3,752,314 + AO356_16630 0.46 -3.0 3,752,314 + AO356_16630 0.46 -0.8 3,752,314 + AO356_16630 0.46 -1.5 3,752,314 + AO356_16630 0.46 -1.6 3,752,314 + AO356_16630 0.46 -0.4 3,752,315 - AO356_16630 0.46 +0.4 3,752,315 - AO356_16630 0.46 +1.0 3,752,315 - AO356_16630 0.46 -1.0 3,752,315 - AO356_16630 0.46 +0.5 3,752,436 - +0.6 3,752,436 - +0.2 3,752,594 + AO356_16635 0.66 +0.4 3,752,595 - AO356_16635 0.66 +1.9 3,752,595 - AO356_16635 0.66 +0.6 3,752,618 + AO356_16635 0.75 +0.2 3,752,619 - AO356_16635 0.75 +0.5 3,752,619 - AO356_16635 0.75 +0.2 3,752,619 - AO356_16635 0.75 -1.3 3,752,619 - AO356_16635 0.75 +0.7 3,752,660 + +0.1 3,752,681 + +1.5 3,752,681 + -0.5 3,752,735 + -1.2 3,752,736 - -1.1 3,752,809 + AO356_16640 0.14 -1.4 3,753,295 + +0.4 3,753,296 - -0.2 3,753,479 - -0.7
Or see this region's nucleotide sequence