Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_14075 and AO356_14080 overlap by 4 nucleotides AO356_14080 and AO356_14085 are separated by 226 nucleotides AO356_14085 and AO356_14090 overlap by 4 nucleotides
AO356_14075: AO356_14075 - amino acid ABC transporter substrate-binding protein, at 3,195,443 to 3,196,414
_14075
AO356_14080: AO356_14080 - RNA polymerase subunit sigma, at 3,196,411 to 3,196,929
_14080
AO356_14085: AO356_14085 - DNA polymerase III subunit epsilon, at 3,197,156 to 3,197,863
_14085
AO356_14090: AO356_14090 - cyclic nucleotide-binding protein, at 3,197,860 to 3,199,800
_14090
Position (kb)
3196
3197 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3196.089 kb on + strand, within AO356_14075 at 3196.089 kb on + strand, within AO356_14075 at 3196.427 kb on + strand at 3196.427 kb on + strand at 3196.428 kb on - strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.439 kb on + strand at 3196.440 kb on - strand at 3196.440 kb on - strand at 3196.440 kb on - strand at 3196.440 kb on - strand at 3196.440 kb on - strand at 3196.440 kb on - strand at 3196.466 kb on + strand, within AO356_14080 at 3196.466 kb on + strand, within AO356_14080 at 3196.466 kb on + strand, within AO356_14080 at 3196.467 kb on - strand, within AO356_14080 at 3196.467 kb on - strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.541 kb on + strand, within AO356_14080 at 3196.542 kb on - strand, within AO356_14080 at 3196.542 kb on - strand, within AO356_14080 at 3196.542 kb on - strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.676 kb on + strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.677 kb on - strand, within AO356_14080 at 3196.706 kb on + strand, within AO356_14080 at 3196.706 kb on + strand, within AO356_14080 at 3196.883 kb on + strand at 3196.883 kb on + strand at 3196.883 kb on + strand at 3196.884 kb on - strand at 3196.884 kb on - strand at 3196.914 kb on + strand at 3197.024 kb on + strand at 3197.108 kb on + strand at 3197.108 kb on + strand at 3197.108 kb on + strand at 3197.109 kb on - strand at 3197.109 kb on - strand at 3197.141 kb on + strand at 3197.141 kb on + strand at 3197.141 kb on + strand at 3197.141 kb on + strand at 3197.142 kb on - strand at 3197.142 kb on - strand at 3197.626 kb on + strand, within AO356_14085 at 3197.626 kb on + strand, within AO356_14085 at 3197.626 kb on + strand, within AO356_14085 at 3197.626 kb on + strand, within AO356_14085 at 3197.626 kb on + strand, within AO356_14085 at 3197.627 kb on - strand, within AO356_14085 at 3197.627 kb on - strand, within AO356_14085 at 3197.627 kb on - strand, within AO356_14085 at 3197.870 kb on + strand at 3197.870 kb on + strand at 3197.870 kb on + strand at 3197.871 kb on - strand at 3197.871 kb on - strand at 3197.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,196,089 + AO356_14075 0.66 -1.1 3,196,089 + AO356_14075 0.66 -0.2 3,196,427 + -0.6 3,196,427 + -0.1 3,196,428 - -0.8 3,196,439 + -0.5 3,196,439 + -0.1 3,196,439 + -0.8 3,196,439 + +0.3 3,196,439 + -0.7 3,196,439 + -0.8 3,196,439 + +1.0 3,196,439 + -0.5 3,196,439 + -0.5 3,196,439 + +2.5 3,196,440 - -0.2 3,196,440 - -0.1 3,196,440 - -1.1 3,196,440 - +0.2 3,196,440 - +0.7 3,196,440 - -1.4 3,196,466 + AO356_14080 0.11 -2.2 3,196,466 + AO356_14080 0.11 -1.4 3,196,466 + AO356_14080 0.11 +2.3 3,196,467 - AO356_14080 0.11 +0.9 3,196,467 - AO356_14080 0.11 -1.5 3,196,541 + AO356_14080 0.25 -0.9 3,196,541 + AO356_14080 0.25 -2.1 3,196,541 + AO356_14080 0.25 -0.5 3,196,541 + AO356_14080 0.25 -1.2 3,196,541 + AO356_14080 0.25 +1.4 3,196,541 + AO356_14080 0.25 -1.1 3,196,541 + AO356_14080 0.25 -0.4 3,196,542 - AO356_14080 0.25 -0.1 3,196,542 - AO356_14080 0.25 -0.2 3,196,542 - AO356_14080 0.25 +0.1 3,196,676 + AO356_14080 0.51 -0.0 3,196,676 + AO356_14080 0.51 -0.2 3,196,676 + AO356_14080 0.51 -0.9 3,196,676 + AO356_14080 0.51 +0.4 3,196,676 + AO356_14080 0.51 +0.4 3,196,676 + AO356_14080 0.51 +0.7 3,196,677 - AO356_14080 0.51 -0.9 3,196,677 - AO356_14080 0.51 -0.4 3,196,677 - AO356_14080 0.51 -1.2 3,196,677 - AO356_14080 0.51 +0.1 3,196,677 - AO356_14080 0.51 -0.8 3,196,677 - AO356_14080 0.51 +0.9 3,196,677 - AO356_14080 0.51 -1.1 3,196,677 - AO356_14080 0.51 +0.5 3,196,677 - AO356_14080 0.51 -0.7 3,196,677 - AO356_14080 0.51 +0.7 3,196,677 - AO356_14080 0.51 +0.3 3,196,677 - AO356_14080 0.51 -1.0 3,196,677 - AO356_14080 0.51 +0.2 3,196,677 - AO356_14080 0.51 +2.6 3,196,706 + AO356_14080 0.57 -1.0 3,196,706 + AO356_14080 0.57 +0.5 3,196,883 + -0.4 3,196,883 + +0.0 3,196,883 + -0.4 3,196,884 - +1.0 3,196,884 - +1.0 3,196,914 + -1.2 3,197,024 + +0.1 3,197,108 + -0.1 3,197,108 + -0.0 3,197,108 + +0.4 3,197,109 - -0.2 3,197,109 - +1.7 3,197,141 + -0.2 3,197,141 + +0.4 3,197,141 + +0.8 3,197,141 + +0.1 3,197,142 - +0.1 3,197,142 - -0.4 3,197,626 + AO356_14085 0.66 +0.6 3,197,626 + AO356_14085 0.66 +0.5 3,197,626 + AO356_14085 0.66 -0.4 3,197,626 + AO356_14085 0.66 -0.0 3,197,626 + AO356_14085 0.66 -0.8 3,197,627 - AO356_14085 0.67 +0.1 3,197,627 - AO356_14085 0.67 +0.2 3,197,627 - AO356_14085 0.67 +1.5 3,197,870 + +0.5 3,197,870 + +1.1 3,197,870 + -1.6 3,197,871 - +0.8 3,197,871 - -0.0 3,197,871 - +0.3
Or see this region's nucleotide sequence