Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02650 and AO356_02655 are separated by 128 nucleotides AO356_02655 and AO356_02660 overlap by 4 nucleotides AO356_02660 and AO356_02665 are separated by 263 nucleotides AO356_02665 and AO356_02670 are separated by 79 nucleotides
AO356_02650: AO356_02650 - type III secretion system protein PrgH, at 582,455 to 583,633
_02650
AO356_02655: AO356_02655 - invasion protein IagB, at 583,762 to 584,226
_02655
AO356_02660: AO356_02660 - invasion protein regulator, at 584,223 to 585,482
_02660
AO356_02665: AO356_02665 - hypothetical protein, at 585,746 to 586,003
_02665
AO356_02670: AO356_02670 - hypothetical protein, at 586,083 to 587,189
_02670
Position (kb)
584
585
586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 583.317 kb on + strand, within AO356_02650 at 583.317 kb on + strand, within AO356_02650 at 583.317 kb on + strand, within AO356_02650 at 583.317 kb on + strand, within AO356_02650 at 583.317 kb on + strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.318 kb on - strand, within AO356_02650 at 583.671 kb on + strand at 583.671 kb on + strand at 583.672 kb on - strand at 583.672 kb on - strand at 583.859 kb on + strand, within AO356_02655 at 583.859 kb on + strand, within AO356_02655 at 583.881 kb on + strand, within AO356_02655 at 583.881 kb on + strand, within AO356_02655 at 583.882 kb on - strand, within AO356_02655 at 583.882 kb on - strand, within AO356_02655 at 583.882 kb on - strand, within AO356_02655 at 583.882 kb on - strand, within AO356_02655 at 583.883 kb on + strand, within AO356_02655 at 583.883 kb on + strand, within AO356_02655 at 583.884 kb on - strand, within AO356_02655 at 584.384 kb on - strand, within AO356_02660 at 584.466 kb on - strand, within AO356_02660 at 584.722 kb on + strand, within AO356_02660 at 584.740 kb on + strand, within AO356_02660 at 584.778 kb on + strand, within AO356_02660 at 584.779 kb on - strand, within AO356_02660 at 584.807 kb on + strand, within AO356_02660 at 584.808 kb on - strand, within AO356_02660 at 585.151 kb on - strand, within AO356_02660 at 585.212 kb on - strand, within AO356_02660 at 585.247 kb on + strand, within AO356_02660 at 585.248 kb on - strand, within AO356_02660 at 585.248 kb on - strand, within AO356_02660 at 585.248 kb on - strand, within AO356_02660 at 585.251 kb on + strand, within AO356_02660 at 585.252 kb on - strand, within AO356_02660 at 585.252 kb on - strand, within AO356_02660 at 585.252 kb on - strand, within AO356_02660 at 585.357 kb on + strand at 585.358 kb on - strand at 585.482 kb on + strand at 585.482 kb on + strand at 585.483 kb on - strand at 585.498 kb on + strand at 585.568 kb on + strand at 585.569 kb on - strand at 585.608 kb on + strand at 585.666 kb on + strand at 585.673 kb on + strand at 585.715 kb on - strand at 585.736 kb on + strand at 585.737 kb on - strand at 585.754 kb on + strand at 585.761 kb on + strand at 585.762 kb on - strand at 585.762 kb on - strand at 585.763 kb on + strand at 585.763 kb on + strand at 585.764 kb on - strand at 585.764 kb on - strand at 585.764 kb on - strand at 585.764 kb on - strand at 585.785 kb on + strand, within AO356_02665 at 585.785 kb on + strand, within AO356_02665 at 585.786 kb on - strand, within AO356_02665 at 585.847 kb on + strand, within AO356_02665 at 585.848 kb on - strand, within AO356_02665 at 585.851 kb on - strand, within AO356_02665 at 585.851 kb on - strand, within AO356_02665 at 585.851 kb on - strand, within AO356_02665 at 585.851 kb on - strand, within AO356_02665 at 585.947 kb on - strand, within AO356_02665 at 585.980 kb on - strand at 586.063 kb on - strand at 586.084 kb on + strand at 586.109 kb on + strand at 586.145 kb on + strand at 586.162 kb on + strand at 586.276 kb on - strand, within AO356_02670 at 586.276 kb on - strand, within AO356_02670 at 586.289 kb on - strand, within AO356_02670
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 583,317 + AO356_02650 0.73 -1.3 583,317 + AO356_02650 0.73 -0.3 583,317 + AO356_02650 0.73 -0.0 583,317 + AO356_02650 0.73 -0.9 583,317 + AO356_02650 0.73 -0.8 583,318 - AO356_02650 0.73 -1.8 583,318 - AO356_02650 0.73 +0.3 583,318 - AO356_02650 0.73 -1.2 583,318 - AO356_02650 0.73 -0.6 583,318 - AO356_02650 0.73 -0.4 583,318 - AO356_02650 0.73 -0.2 583,671 + -1.1 583,671 + -1.0 583,672 - -2.2 583,672 - -1.5 583,859 + AO356_02655 0.21 +0.5 583,859 + AO356_02655 0.21 -1.3 583,881 + AO356_02655 0.26 -0.7 583,881 + AO356_02655 0.26 +0.6 583,882 - AO356_02655 0.26 -0.8 583,882 - AO356_02655 0.26 +0.1 583,882 - AO356_02655 0.26 +0.3 583,882 - AO356_02655 0.26 +1.1 583,883 + AO356_02655 0.26 -1.0 583,883 + AO356_02655 0.26 +0.6 583,884 - AO356_02655 0.26 +0.2 584,384 - AO356_02660 0.13 -1.0 584,466 - AO356_02660 0.19 +0.4 584,722 + AO356_02660 0.40 +0.8 584,740 + AO356_02660 0.41 +0.0 584,778 + AO356_02660 0.44 +0.1 584,779 - AO356_02660 0.44 -0.0 584,807 + AO356_02660 0.46 +0.8 584,808 - AO356_02660 0.46 -0.3 585,151 - AO356_02660 0.74 +0.7 585,212 - AO356_02660 0.78 +0.1 585,247 + AO356_02660 0.81 -1.6 585,248 - AO356_02660 0.81 +2.1 585,248 - AO356_02660 0.81 +0.3 585,248 - AO356_02660 0.81 +0.3 585,251 + AO356_02660 0.82 -0.3 585,252 - AO356_02660 0.82 -0.8 585,252 - AO356_02660 0.82 -1.0 585,252 - AO356_02660 0.82 -0.5 585,357 + -1.1 585,358 - -0.1 585,482 + -0.5 585,482 + -1.1 585,483 - +0.3 585,498 + -0.7 585,568 + +0.5 585,569 - +1.1 585,608 + +0.3 585,666 + -1.2 585,673 + -0.3 585,715 - -0.2 585,736 + -1.1 585,737 - -0.1 585,754 + -1.8 585,761 + -1.7 585,762 - -1.8 585,762 - -1.3 585,763 + -0.1 585,763 + -1.4 585,764 - +0.9 585,764 - +0.0 585,764 - -1.3 585,764 - -0.1 585,785 + AO356_02665 0.15 -0.5 585,785 + AO356_02665 0.15 -0.3 585,786 - AO356_02665 0.16 -0.8 585,847 + AO356_02665 0.39 +1.1 585,848 - AO356_02665 0.40 -0.2 585,851 - AO356_02665 0.41 -0.9 585,851 - AO356_02665 0.41 -0.5 585,851 - AO356_02665 0.41 +0.3 585,851 - AO356_02665 0.41 +1.0 585,947 - AO356_02665 0.78 -0.3 585,980 - -1.0 586,063 - +1.3 586,084 + +0.6 586,109 + -1.3 586,145 + +1.4 586,162 + -0.1 586,276 - AO356_02670 0.17 -1.5 586,276 - AO356_02670 0.17 -0.8 586,289 - AO356_02670 0.19 +0.5
Or see this region's nucleotide sequence