Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02315 and AO356_02320 are separated by 115 nucleotides AO356_02320 and AO356_02325 are separated by 108 nucleotides AO356_02325 and AO356_02330 are separated by 30 nucleotides
AO356_02315: AO356_02315 - DNA topoisomerase I, at 499,240 to 501,852
_02315
AO356_02320: AO356_02320 - TonB box-like protein, at 501,968 to 502,204
_02320
AO356_02325: AO356_02325 - 3-ketoacyl-CoA thiolase, at 502,313 to 503,488
_02325
AO356_02330: AO356_02330 - multifunctional fatty acid oxidation complex subunit alpha, at 503,519 to 505,666
_02330
Position (kb) 502
503
504 Strain fitness (log2 ratio) -2
-1
0
1 at 501.915 kb on - strand at 501.915 kb on - strand at 501.915 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.917 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.919 kb on - strand at 501.921 kb on - strand at 501.921 kb on - strand at 502.092 kb on + strand, within AO356_02320 at 502.093 kb on - strand, within AO356_02320 at 502.104 kb on + strand, within AO356_02320 at 502.104 kb on + strand, within AO356_02320 at 502.105 kb on - strand, within AO356_02320 at 502.151 kb on + strand, within AO356_02320 at 502.151 kb on + strand, within AO356_02320 at 502.152 kb on - strand, within AO356_02320 at 502.152 kb on - strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.173 kb on + strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.174 kb on - strand, within AO356_02320 at 502.196 kb on + strand at 502.252 kb on + strand at 502.253 kb on - strand at 502.255 kb on + strand at 502.314 kb on + strand at 502.424 kb on - strand at 502.481 kb on - strand, within AO356_02325 at 502.633 kb on - strand, within AO356_02325 at 502.861 kb on - strand, within AO356_02325 at 503.473 kb on + strand at 503.489 kb on - strand at 503.489 kb on - strand at 503.489 kb on - strand at 503.489 kb on - strand at 503.489 kb on - strand at 503.505 kb on + strand at 503.599 kb on - strand at 503.689 kb on - strand at 503.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 501,915 - +0.6 501,915 - -0.0 501,915 - -1.3 501,917 - +0.2 501,917 - +0.6 501,917 - +0.4 501,917 - +1.3 501,917 - +0.1 501,917 - -1.6 501,917 - -0.3 501,917 - -0.4 501,917 - -1.0 501,917 - +0.8 501,919 - +0.1 501,919 - +0.5 501,919 - +0.1 501,919 - +0.1 501,919 - +1.4 501,919 - -1.5 501,919 - +0.2 501,919 - -0.4 501,919 - +0.4 501,919 - +0.5 501,919 - -0.1 501,919 - -0.4 501,919 - +0.1 501,919 - -0.5 501,919 - +0.7 501,919 - -0.0 501,919 - -2.2 501,919 - -0.1 501,921 - +0.6 501,921 - -1.4 502,092 + AO356_02320 0.52 +0.4 502,093 - AO356_02320 0.53 +0.6 502,104 + AO356_02320 0.57 -0.0 502,104 + AO356_02320 0.57 -0.7 502,105 - AO356_02320 0.58 +0.1 502,151 + AO356_02320 0.77 +0.4 502,151 + AO356_02320 0.77 -0.4 502,152 - AO356_02320 0.78 +0.1 502,152 - AO356_02320 0.78 -0.7 502,173 + AO356_02320 0.86 +0.4 502,173 + AO356_02320 0.86 +0.8 502,173 + AO356_02320 0.86 +0.3 502,173 + AO356_02320 0.86 -0.6 502,173 + AO356_02320 0.86 -0.0 502,173 + AO356_02320 0.86 +0.6 502,173 + AO356_02320 0.86 +0.6 502,174 - AO356_02320 0.87 -0.4 502,174 - AO356_02320 0.87 -1.5 502,174 - AO356_02320 0.87 -1.3 502,196 + -0.3 502,252 + +0.3 502,253 - -1.7 502,255 + +1.2 502,314 + +1.3 502,424 - -2.3 502,481 - AO356_02325 0.14 -2.7 502,633 - AO356_02325 0.27 -0.6 502,861 - AO356_02325 0.47 -0.1 503,473 + +0.1 503,489 - +0.3 503,489 - +0.1 503,489 - -2.6 503,489 - -1.0 503,489 - -2.0 503,505 + -1.5 503,599 - +0.5 503,689 - -0.1 503,689 - -0.6
Or see this region's nucleotide sequence