Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_00280 and AO356_00285 are separated by 460 nucleotides AO356_00285 and AO356_00290 are separated by 130 nucleotides AO356_00290 and AO356_00295 are separated by 132 nucleotides AO356_00295 and AO356_00300 are separated by 229 nucleotides
AO356_00280: AO356_00280 - hypothetical protein, at 51,605 to 52,000
_00280
AO356_00285: AO356_00285 - hypothetical protein, at 52,461 to 52,790
_00285
AO356_00290: AO356_00290 - DNA primase, at 52,921 to 53,883
_00290
AO356_00295: AO356_00295 - hypothetical protein, at 54,016 to 54,210
_00295
AO356_00300: AO356_00300 - relaxase, at 54,440 to 56,164
_00300
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.940 kb on + strand, within AO356_00280 at 51.941 kb on - strand, within AO356_00280 at 51.951 kb on + strand, within AO356_00280 at 51.951 kb on + strand, within AO356_00280 at 52.022 kb on + strand at 52.050 kb on + strand at 52.050 kb on + strand at 52.050 kb on + strand at 52.050 kb on + strand at 52.050 kb on + strand at 52.051 kb on - strand at 52.051 kb on - strand at 52.051 kb on - strand at 52.051 kb on - strand at 52.051 kb on - strand at 52.053 kb on + strand at 52.149 kb on + strand at 52.170 kb on - strand at 52.170 kb on - strand at 52.180 kb on + strand at 52.180 kb on + strand at 52.180 kb on + strand at 52.181 kb on - strand at 52.181 kb on - strand at 52.184 kb on + strand at 52.191 kb on + strand at 52.245 kb on - strand at 52.245 kb on - strand at 52.312 kb on - strand at 52.319 kb on + strand at 52.455 kb on + strand at 52.455 kb on + strand at 52.455 kb on + strand at 52.455 kb on + strand at 52.456 kb on - strand at 52.456 kb on - strand at 52.471 kb on + strand at 52.543 kb on + strand, within AO356_00285 at 52.544 kb on - strand, within AO356_00285 at 52.647 kb on + strand, within AO356_00285 at 52.647 kb on + strand, within AO356_00285 at 52.647 kb on + strand, within AO356_00285 at 52.648 kb on - strand, within AO356_00285 at 52.648 kb on - strand, within AO356_00285 at 52.683 kb on - strand, within AO356_00285 at 52.749 kb on - strand, within AO356_00285 at 52.831 kb on - strand at 52.831 kb on - strand at 52.832 kb on + strand at 52.832 kb on + strand at 52.930 kb on - strand at 52.930 kb on - strand at 52.933 kb on + strand at 52.933 kb on + strand at 52.933 kb on + strand at 52.934 kb on - strand at 52.934 kb on - strand at 52.968 kb on - strand at 53.213 kb on - strand, within AO356_00290 at 53.213 kb on - strand, within AO356_00290 at 53.426 kb on + strand, within AO356_00290 at 53.426 kb on + strand, within AO356_00290 at 53.426 kb on + strand, within AO356_00290 at 53.427 kb on - strand, within AO356_00290 at 53.442 kb on + strand, within AO356_00290 at 53.442 kb on + strand, within AO356_00290 at 53.537 kb on - strand, within AO356_00290 at 53.539 kb on + strand, within AO356_00290 at 53.539 kb on + strand, within AO356_00290 at 53.540 kb on - strand, within AO356_00290 at 53.638 kb on + strand, within AO356_00290 at 53.682 kb on - strand, within AO356_00290 at 53.689 kb on + strand, within AO356_00290 at 53.690 kb on - strand, within AO356_00290 at 53.809 kb on + strand at 54.042 kb on - strand, within AO356_00295 at 54.042 kb on - strand, within AO356_00295 at 54.083 kb on + strand, within AO356_00295 at 54.083 kb on + strand, within AO356_00295 at 54.165 kb on + strand, within AO356_00295 at 54.165 kb on + strand, within AO356_00295 at 54.166 kb on - strand, within AO356_00295 at 54.166 kb on - strand, within AO356_00295 at 54.184 kb on + strand, within AO356_00295 at 54.213 kb on + strand at 54.214 kb on - strand at 54.214 kb on - strand at 54.214 kb on - strand at 54.263 kb on + strand at 54.300 kb on + strand at 54.300 kb on + strand at 54.301 kb on - strand at 54.306 kb on + strand at 54.380 kb on + strand at 54.402 kb on + strand at 54.509 kb on + strand at 54.510 kb on - strand at 54.510 kb on - strand at 54.510 kb on - strand at 54.585 kb on - strand at 54.585 kb on - strand at 54.620 kb on + strand, within AO356_00300 at 54.620 kb on + strand, within AO356_00300 at 54.620 kb on + strand, within AO356_00300 at 54.625 kb on - strand, within AO356_00300 at 54.625 kb on - strand, within AO356_00300 at 54.625 kb on - strand, within AO356_00300 at 54.642 kb on - strand, within AO356_00300 at 54.692 kb on + strand, within AO356_00300 at 54.693 kb on - strand, within AO356_00300 at 54.758 kb on + strand, within AO356_00300 at 54.759 kb on - strand, within AO356_00300
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 51,940 + AO356_00280 0.85 +0.6 51,941 - AO356_00280 0.85 -0.9 51,951 + AO356_00280 0.87 +1.0 51,951 + AO356_00280 0.87 +0.4 52,022 + +0.2 52,050 + +0.3 52,050 + -1.1 52,050 + -0.8 52,050 + -0.4 52,050 + +0.7 52,051 - +0.5 52,051 - +1.9 52,051 - +0.2 52,051 - -0.1 52,051 - +1.3 52,053 + -0.8 52,149 + -0.7 52,170 - -0.3 52,170 - +1.0 52,180 + +0.5 52,180 + +2.3 52,180 + +1.5 52,181 - +0.0 52,181 - +0.3 52,184 + -1.0 52,191 + -1.3 52,245 - -0.7 52,245 - +0.3 52,312 - +0.5 52,319 + +0.3 52,455 + -0.6 52,455 + +0.2 52,455 + +0.9 52,455 + -0.1 52,456 - -2.4 52,456 - -0.3 52,471 + +0.1 52,543 + AO356_00285 0.25 -1.6 52,544 - AO356_00285 0.25 +0.9 52,647 + AO356_00285 0.56 -2.2 52,647 + AO356_00285 0.56 +0.3 52,647 + AO356_00285 0.56 -0.4 52,648 - AO356_00285 0.57 -1.1 52,648 - AO356_00285 0.57 -1.1 52,683 - AO356_00285 0.67 +0.6 52,749 - AO356_00285 0.87 +0.8 52,831 - -1.6 52,831 - -0.3 52,832 + +0.3 52,832 + -0.0 52,930 - +0.3 52,930 - -0.8 52,933 + +0.9 52,933 + -0.3 52,933 + -0.0 52,934 - +2.0 52,934 - -2.3 52,968 - -1.9 53,213 - AO356_00290 0.30 -1.8 53,213 - AO356_00290 0.30 -1.3 53,426 + AO356_00290 0.52 +0.1 53,426 + AO356_00290 0.52 +0.6 53,426 + AO356_00290 0.52 +0.3 53,427 - AO356_00290 0.53 +0.1 53,442 + AO356_00290 0.54 -0.7 53,442 + AO356_00290 0.54 +0.7 53,537 - AO356_00290 0.64 +0.0 53,539 + AO356_00290 0.64 -0.4 53,539 + AO356_00290 0.64 -1.0 53,540 - AO356_00290 0.64 -0.5 53,638 + AO356_00290 0.74 -0.4 53,682 - AO356_00290 0.79 +0.3 53,689 + AO356_00290 0.80 +0.1 53,690 - AO356_00290 0.80 -0.7 53,809 + +0.1 54,042 - AO356_00295 0.13 -0.7 54,042 - AO356_00295 0.13 +0.1 54,083 + AO356_00295 0.34 -0.4 54,083 + AO356_00295 0.34 +2.0 54,165 + AO356_00295 0.76 -0.7 54,165 + AO356_00295 0.76 +0.8 54,166 - AO356_00295 0.77 +0.7 54,166 - AO356_00295 0.77 +0.4 54,184 + AO356_00295 0.86 -0.5 54,213 + -0.0 54,214 - -0.0 54,214 - +0.8 54,214 - +0.3 54,263 + -0.3 54,300 + +0.6 54,300 + +2.5 54,301 - +0.1 54,306 + +0.6 54,380 + +0.9 54,402 + -0.9 54,509 + +0.4 54,510 - -0.7 54,510 - +0.5 54,510 - -0.3 54,585 - -0.3 54,585 - +0.7 54,620 + AO356_00300 0.10 -0.1 54,620 + AO356_00300 0.10 +0.1 54,620 + AO356_00300 0.10 -0.8 54,625 - AO356_00300 0.11 -1.0 54,625 - AO356_00300 0.11 +2.3 54,625 - AO356_00300 0.11 +0.1 54,642 - AO356_00300 0.12 +0.0 54,692 + AO356_00300 0.15 +0.7 54,693 - AO356_00300 0.15 -1.3 54,758 + AO356_00300 0.18 +0.2 54,759 - AO356_00300 0.18 +1.6
Or see this region's nucleotide sequence