Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0346 and DvMF_0347 are separated by 163 nucleotides DvMF_0347 and DvMF_0348 are separated by 199 nucleotides DvMF_0348 and secG are separated by 38 nucleotides secG and DvMF_R0017 are separated by 36 nucleotides DvMF_R0017 and DvMF_0350 are separated by 200 nucleotides
DvMF_0346: DvMF_0346 - NUDIX hydrolase (RefSeq), at 405,236 to 405,784
_0346
DvMF_0347: DvMF_0347 - ribosomal-protein-alanine acetyltransferase (RefSeq), at 405,948 to 406,526
_0347
DvMF_0348: DvMF_0348 - Triose-phosphate isomerase (RefSeq), at 406,726 to 407,481
_0348
DvMF_0349: secG - preprotein translocase subunit SecG (RefSeq), at 407,520 to 407,864
secG
DvMF_R0017: DvMF_R0017 - tRNA-Leu (RefSeq), at 407,901 to 407,987
_R0017
DvMF_0350: DvMF_0350 - extracellular solute-binding protein family 3 (RefSeq), at 408,188 to 409,153
_0350
Position (kb) 406
407
408 Strain fitness (log2 ratio) -2
-1
0
1 at 405.809 kb on - strand at 405.809 kb on - strand at 405.953 kb on + strand at 406.241 kb on - strand, within DvMF_0347 at 406.473 kb on - strand at 406.511 kb on + strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.646 kb on - strand at 406.696 kb on + strand at 406.714 kb on + strand at 407.179 kb on + strand, within DvMF_0348 at 407.238 kb on + strand, within DvMF_0348 at 408.258 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 405,809 - -0.2 405,809 - +0.6 405,953 + -1.2 406,241 - DvMF_0347 0.51 -0.6 406,473 - -0.3 406,511 + -0.7 406,529 - -0.9 406,529 - -0.8 406,646 - -0.5 406,696 + -0.3 406,714 + -2.1 407,179 + DvMF_0348 0.60 -0.9 407,238 + DvMF_0348 0.68 -0.9 408,258 + -1.4
Or see this region's nucleotide sequence