Experiment: Carbenicillin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2299 and Shew_2300 are separated by 151 nucleotides Shew_2300 and Shew_2301 are separated by 152 nucleotides Shew_2301 and Shew_2302 are separated by 188 nucleotides
Shew_2299: Shew_2299 - DNA topoisomerase III (RefSeq), at 2,692,559 to 2,694,571
_2299
Shew_2300: Shew_2300 - hypothetical protein (RefSeq), at 2,694,723 to 2,694,998
_2300
Shew_2301: Shew_2301 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 2,695,151 to 2,697,268
_2301
Shew_2302: Shew_2302 - amidophosphoribosyltransferase (RefSeq), at 2,697,457 to 2,698,971
_2302
Position (kb)
2695
2696
2697
2698 Strain fitness (log2 ratio)
-1
0
1 at 2694.348 kb on + strand, within Shew_2299 at 2694.955 kb on + strand, within Shew_2300 at 2695.425 kb on + strand, within Shew_2301 at 2695.458 kb on + strand, within Shew_2301 at 2695.667 kb on - strand, within Shew_2301 at 2695.667 kb on - strand, within Shew_2301 at 2695.771 kb on - strand, within Shew_2301 at 2696.252 kb on - strand, within Shew_2301 at 2696.933 kb on - strand, within Shew_2301 at 2696.976 kb on - strand, within Shew_2301 at 2697.378 kb on - strand at 2697.870 kb on + strand, within Shew_2302 at 2697.989 kb on + strand, within Shew_2302
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.002 mg/ml remove 2,694,348 + Shew_2299 0.89 -0.2 2,694,955 + Shew_2300 0.84 +0.1 2,695,425 + Shew_2301 0.13 +0.2 2,695,458 + Shew_2301 0.14 +0.4 2,695,667 - Shew_2301 0.24 -0.4 2,695,667 - Shew_2301 0.24 +0.1 2,695,771 - Shew_2301 0.29 +0.0 2,696,252 - Shew_2301 0.52 +0.8 2,696,933 - Shew_2301 0.84 +1.2 2,696,976 - Shew_2301 0.86 +0.4 2,697,378 - +0.1 2,697,870 + Shew_2302 0.27 -1.4 2,697,989 + Shew_2302 0.35 -0.2
Or see this region's nucleotide sequence