Experiment: Carbenicillin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2112 and Shew_2113 are separated by 44 nucleotides Shew_2113 and Shew_2114 are separated by 137 nucleotides Shew_2114 and Shew_2115 are separated by 95 nucleotides
Shew_2112: Shew_2112 - ribonuclease H (RefSeq), at 2,447,746 to 2,448,222
_2112
Shew_2113: Shew_2113 - hypothetical protein (RefSeq), at 2,448,267 to 2,448,989
_2113
Shew_2114: Shew_2114 - hydroxyacylglutathione hydrolase (RefSeq), at 2,449,127 to 2,449,909
_2114
Shew_2115: Shew_2115 - lytic transglycosylase, catalytic (RefSeq), at 2,450,005 to 2,451,540
_2115
Position (kb)
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2448.274 kb on + strand at 2448.985 kb on - strand at 2449.243 kb on - strand, within Shew_2114 at 2449.411 kb on + strand, within Shew_2114 at 2449.484 kb on - strand, within Shew_2114 at 2449.484 kb on - strand, within Shew_2114 at 2449.529 kb on - strand, within Shew_2114 at 2449.959 kb on - strand at 2450.147 kb on - strand at 2450.281 kb on - strand, within Shew_2115 at 2450.301 kb on - strand, within Shew_2115 at 2450.525 kb on - strand, within Shew_2115 at 2450.611 kb on + strand, within Shew_2115 at 2450.661 kb on - strand, within Shew_2115 at 2450.682 kb on + strand, within Shew_2115 at 2450.780 kb on - strand, within Shew_2115
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.002 mg/ml remove 2,448,274 + +0.2 2,448,985 - +0.0 2,449,243 - Shew_2114 0.15 -0.0 2,449,411 + Shew_2114 0.36 +1.0 2,449,484 - Shew_2114 0.46 +1.1 2,449,484 - Shew_2114 0.46 -0.0 2,449,529 - Shew_2114 0.51 -0.5 2,449,959 - -2.8 2,450,147 - -2.8 2,450,281 - Shew_2115 0.18 -2.8 2,450,301 - Shew_2115 0.19 -0.6 2,450,525 - Shew_2115 0.34 -1.9 2,450,611 + Shew_2115 0.39 -3.0 2,450,661 - Shew_2115 0.43 -3.4 2,450,682 + Shew_2115 0.44 -1.0 2,450,780 - Shew_2115 0.50 -1.5
Or see this region's nucleotide sequence