Experiment: Carbenicillin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0078 and Shew_R0079 are separated by 93 nucleotides Shew_R0079 and Shew_R0080 are separated by 49 nucleotides Shew_R0080 and Shew_2110 are separated by 117 nucleotides Shew_2110 and Shew_2111 are separated by 10 nucleotides Shew_2111 and Shew_2112 are separated by 68 nucleotides
Shew_R0078: Shew_R0078 - tRNA-OTHER (NCBI), at 2,445,114 to 2,445,202
_R0078
Shew_R0079: Shew_R0079 - tRNA-Asp (RefSeq), at 2,445,296 to 2,445,372
_R0079
Shew_R0080: Shew_R0080 - tRNA-Asp (RefSeq), at 2,445,422 to 2,445,498
_R0080
Shew_2110: Shew_2110 - hypothetical protein (RefSeq), at 2,445,616 to 2,446,938
_2110
Shew_2111: Shew_2111 - DNA polymerase III, epsilon subunit (RefSeq), at 2,446,949 to 2,447,677
_2111
Shew_2112: Shew_2112 - ribonuclease H (RefSeq), at 2,447,746 to 2,448,222
_2112
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-1
0
1 at 2444.661 kb on + strand at 2445.818 kb on - strand, within Shew_2110 at 2445.824 kb on + strand, within Shew_2110 at 2445.824 kb on + strand, within Shew_2110 at 2445.832 kb on - strand, within Shew_2110 at 2445.905 kb on - strand, within Shew_2110 at 2446.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.002 mg/ml remove 2,444,661 + +0.1 2,445,818 - Shew_2110 0.15 +0.7 2,445,824 + Shew_2110 0.16 +0.2 2,445,824 + Shew_2110 0.16 +0.1 2,445,832 - Shew_2110 0.16 +0.4 2,445,905 - Shew_2110 0.22 +0.7 2,446,856 - -0.2
Or see this region's nucleotide sequence