Experiment: Carbenicillin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and Shew_2076 are separated by 19 nucleotides Shew_2076 and Shew_2077 overlap by 4 nucleotides Shew_2077 and Shew_2078 are separated by 7 nucleotides Shew_2078 and aspS are separated by 70 nucleotides
Shew_2075: ruvB - Holliday junction DNA helicase B (RefSeq), at 2,399,610 to 2,400,623
ruvB
Shew_2076: Shew_2076 - Holliday junction DNA helicase RuvA (RefSeq), at 2,400,643 to 2,401,260
_2076
Shew_2077: Shew_2077 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 2,401,257 to 2,401,778
_2077
Shew_2078: Shew_2078 - hypothetical protein (RefSeq), at 2,401,786 to 2,402,532
_2078
Shew_2079: aspS - aspartyl-tRNA synthetase (RefSeq), at 2,402,603 to 2,404,381
aspS
Position (kb)
2401
2402 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2400.409 kb on + strand, within ruvB at 2400.409 kb on + strand, within ruvB at 2401.337 kb on + strand, within Shew_2077 at 2401.407 kb on + strand, within Shew_2077 at 2401.719 kb on - strand, within Shew_2077 at 2401.987 kb on - strand, within Shew_2078
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.002 mg/ml remove 2,400,409 + ruvB Shew_2075 0.79 -0.6 2,400,409 + ruvB Shew_2075 0.79 -1.6 2,401,337 + Shew_2077 0.15 -2.4 2,401,407 + Shew_2077 0.29 -2.0 2,401,719 - Shew_2077 0.89 -4.6 2,401,987 - Shew_2078 0.27 -2.4
Or see this region's nucleotide sequence