Experiment: m.b. Cobalt chloride 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3369 and Dshi_3370 are separated by 64 nucleotides Dshi_3370 and Dshi_3371 are separated by 92 nucleotides Dshi_3371 and Dshi_3372 are separated by 227 nucleotides Dshi_3372 and Dshi_3373 are separated by 21 nucleotides
Dshi_3369: Dshi_3369 - formamidopyrimidine-DNA glycosylase (RefSeq), at 3,541,951 to 3,542,805
_3369
Dshi_3370: Dshi_3370 - Enoyl-CoA hydratase/isomerase (RefSeq), at 3,542,870 to 3,543,646
_3370
Dshi_3371: Dshi_3371 - ribosomal protein S20 (RefSeq), at 3,543,739 to 3,544,005
_3371
Dshi_3372: Dshi_3372 - hypothetical protein (RefSeq), at 3,544,233 to 3,544,682
_3372
Dshi_3373: Dshi_3373 - chromosomal replication initiator protein DnaA (RefSeq), at 3,544,704 to 3,546,086
_3373
Position (kb)
3543
3544
3545 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3542.751 kb on - strand at 3542.763 kb on + strand at 3542.771 kb on - strand at 3542.776 kb on + strand at 3542.795 kb on + strand at 3542.805 kb on + strand at 3542.813 kb on - strand at 3542.825 kb on + strand at 3543.655 kb on - strand at 3544.564 kb on + strand, within Dshi_3372
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.1 mM remove 3,542,751 - +0.2 3,542,763 + +1.9 3,542,771 - +0.9 3,542,776 + +1.4 3,542,795 + +1.7 3,542,805 + +0.0 3,542,813 - +0.2 3,542,825 + +0.4 3,543,655 - +1.1 3,544,564 + Dshi_3372 0.74 -2.8
Or see this region's nucleotide sequence