Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4429 and BT4430 are separated by 13 nucleotides BT4430 and BT4431 are separated by 195 nucleotides
BT4429: BT4429 - putative pteridine-dependent dioxygenase (NCBI ptt file), at 5,834,265 to 5,835,410
BT4429
BT4430: BT4430 - cytochrome c biogenesis protein (ccsA) (NCBI ptt file), at 5,835,424 to 5,837,859
BT4430
BT4431: BT4431 - hypothetical protein (NCBI ptt file), at 5,838,055 to 5,839,854
BT4431
Position (kb)
5835
5836
5837
5838 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5834.585 kb on - strand, within BT4429 at 5834.647 kb on + strand, within BT4429 at 5834.835 kb on - strand, within BT4429 at 5834.842 kb on + strand, within BT4429 at 5834.842 kb on + strand, within BT4429 at 5834.842 kb on + strand, within BT4429 at 5834.842 kb on + strand, within BT4429 at 5834.843 kb on - strand, within BT4429 at 5834.843 kb on - strand, within BT4429 at 5834.843 kb on - strand, within BT4429 at 5834.957 kb on - strand, within BT4429 at 5835.108 kb on - strand, within BT4429 at 5835.346 kb on - strand at 5835.361 kb on + strand at 5835.362 kb on - strand at 5835.464 kb on - strand at 5835.481 kb on + strand at 5835.557 kb on - strand at 5835.571 kb on - strand at 5835.702 kb on + strand, within BT4430 at 5835.890 kb on + strand, within BT4430 at 5835.936 kb on + strand, within BT4430 at 5835.937 kb on - strand, within BT4430 at 5836.045 kb on + strand, within BT4430 at 5836.054 kb on + strand, within BT4430 at 5836.242 kb on - strand, within BT4430 at 5836.464 kb on + strand, within BT4430 at 5836.465 kb on - strand, within BT4430 at 5836.534 kb on + strand, within BT4430 at 5836.648 kb on - strand at 5836.740 kb on + strand, within BT4430 at 5836.740 kb on + strand, within BT4430 at 5836.780 kb on - strand, within BT4430 at 5836.780 kb on - strand, within BT4430 at 5836.783 kb on + strand, within BT4430 at 5836.783 kb on + strand, within BT4430 at 5836.783 kb on + strand, within BT4430 at 5836.784 kb on - strand, within BT4430 at 5836.809 kb on + strand, within BT4430 at 5836.810 kb on - strand, within BT4430 at 5836.813 kb on - strand, within BT4430 at 5836.852 kb on - strand, within BT4430 at 5836.852 kb on - strand, within BT4430 at 5836.873 kb on - strand, within BT4430 at 5836.920 kb on + strand, within BT4430 at 5837.043 kb on - strand, within BT4430 at 5837.137 kb on + strand, within BT4430 at 5837.232 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.312 kb on - strand, within BT4430 at 5837.354 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.463 kb on - strand, within BT4430 at 5837.492 kb on + strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.605 kb on + strand, within BT4430 at 5837.650 kb on - strand at 5837.666 kb on - strand at 5837.726 kb on - strand at 5837.851 kb on + strand at 5837.869 kb on + strand at 5837.913 kb on - strand at 5838.006 kb on - strand at 5838.109 kb on + strand at 5838.119 kb on + strand at 5838.175 kb on - strand at 5838.175 kb on - strand at 5838.246 kb on + strand, within BT4431 at 5838.248 kb on + strand, within BT4431 at 5838.324 kb on + strand, within BT4431 at 5838.374 kb on + strand, within BT4431 at 5838.375 kb on - strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.377 kb on - strand, within BT4431 at 5838.379 kb on - strand, within BT4431 at 5838.381 kb on - strand, within BT4431 at 5838.547 kb on - strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.720 kb on - strand, within BT4431 at 5838.745 kb on + strand, within BT4431 at 5838.763 kb on + strand, within BT4431 at 5838.853 kb on - strand, within BT4431
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 5,834,585 - BT4429 0.28 +1.3 5,834,647 + BT4429 0.33 -2.0 5,834,835 - BT4429 0.50 -0.3 5,834,842 + BT4429 0.50 -1.0 5,834,842 + BT4429 0.50 +0.3 5,834,842 + BT4429 0.50 +1.0 5,834,842 + BT4429 0.50 +0.9 5,834,843 - BT4429 0.50 -1.0 5,834,843 - BT4429 0.50 -2.0 5,834,843 - BT4429 0.50 -1.7 5,834,957 - BT4429 0.60 -1.9 5,835,108 - BT4429 0.74 -1.8 5,835,346 - -1.3 5,835,361 + -0.4 5,835,362 - +1.2 5,835,464 - -2.5 5,835,481 + +0.3 5,835,557 - -0.3 5,835,571 - -0.0 5,835,702 + BT4430 0.11 -0.0 5,835,890 + BT4430 0.19 +0.3 5,835,936 + BT4430 0.21 +0.5 5,835,937 - BT4430 0.21 +2.7 5,836,045 + BT4430 0.25 -0.1 5,836,054 + BT4430 0.26 +1.3 5,836,242 - BT4430 0.34 +0.3 5,836,464 + BT4430 0.43 -1.9 5,836,465 - BT4430 0.43 -1.5 5,836,534 + BT4430 0.46 -1.0 5,836,648 - +1.3 5,836,740 + BT4430 0.54 +1.3 5,836,740 + BT4430 0.54 +0.9 5,836,780 - BT4430 0.56 -1.3 5,836,780 - BT4430 0.56 -2.9 5,836,783 + BT4430 0.56 -1.0 5,836,783 + BT4430 0.56 +0.3 5,836,783 + BT4430 0.56 -0.3 5,836,784 - BT4430 0.56 -2.5 5,836,809 + BT4430 0.57 +1.1 5,836,810 - BT4430 0.57 +0.5 5,836,813 - BT4430 0.57 +2.4 5,836,852 - BT4430 0.59 +0.3 5,836,852 - BT4430 0.59 +1.7 5,836,873 - BT4430 0.59 +0.6 5,836,920 + BT4430 0.61 +1.3 5,837,043 - BT4430 0.66 +1.1 5,837,137 + BT4430 0.70 +0.1 5,837,232 - BT4430 0.74 +0.3 5,837,283 - BT4430 0.76 +0.7 5,837,283 - BT4430 0.76 -1.9 5,837,312 - BT4430 0.78 -1.0 5,837,354 + BT4430 0.79 -1.0 5,837,462 + BT4430 0.84 -0.1 5,837,462 + BT4430 0.84 +0.4 5,837,463 - BT4430 0.84 -1.2 5,837,492 + BT4430 0.85 -3.2 5,837,596 - BT4430 0.89 +0.8 5,837,596 - BT4430 0.89 +1.3 5,837,605 + BT4430 0.90 -0.6 5,837,650 - +1.7 5,837,666 - -0.3 5,837,726 - -1.3 5,837,851 + -3.6 5,837,869 + -2.0 5,837,913 - -0.2 5,838,006 - -2.0 5,838,109 + -1.0 5,838,119 + +0.1 5,838,175 - -0.3 5,838,175 - -1.5 5,838,246 + BT4431 0.11 -0.1 5,838,248 + BT4431 0.11 +0.3 5,838,324 + BT4431 0.15 +0.3 5,838,374 + BT4431 0.18 -0.4 5,838,375 - BT4431 0.18 -2.9 5,838,376 + BT4431 0.18 -0.0 5,838,376 + BT4431 0.18 +0.7 5,838,376 + BT4431 0.18 -2.6 5,838,377 - BT4431 0.18 -2.3 5,838,379 - BT4431 0.18 +0.7 5,838,381 - BT4431 0.18 -0.8 5,838,547 - BT4431 0.27 +1.3 5,838,635 + BT4431 0.32 +0.7 5,838,635 + BT4431 0.32 +2.3 5,838,720 - BT4431 0.37 +0.9 5,838,745 + BT4431 0.38 -2.5 5,838,763 + BT4431 0.39 -0.7 5,838,853 - BT4431 0.44 -0.3
Or see this region's nucleotide sequence