Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4324 and BT4325 are separated by 11 nucleotides BT4325 and BT4326 are separated by 45 nucleotides BT4326 and BT4327 are separated by 7 nucleotides
BT4324: BT4324 - conserved hypothetical protein (NCBI ptt file), at 5,695,257 to 5,697,899
BT4324
BT4325: BT4325 - hypothetical protein (NCBI ptt file), at 5,697,911 to 5,699,116
BT4325
BT4326: BT4326 - ABC transporter ATP-binding protein (NCBI ptt file), at 5,699,162 to 5,699,815
BT4326
BT4327: BT4327 - hypothetical protein (NCBI ptt file), at 5,699,823 to 5,701,370
BT4327
Position (kb) 5697
5698
5699
5700 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5696.936 kb on - strand, within BT4324 at 5696.936 kb on - strand, within BT4324 at 5696.938 kb on + strand, within BT4324 at 5696.966 kb on + strand, within BT4324 at 5696.979 kb on - strand, within BT4324 at 5697.306 kb on - strand, within BT4324 at 5697.321 kb on + strand, within BT4324 at 5697.389 kb on - strand, within BT4324 at 5697.389 kb on - strand, within BT4324 at 5697.389 kb on - strand, within BT4324 at 5697.434 kb on + strand, within BT4324 at 5697.435 kb on - strand, within BT4324 at 5697.453 kb on + strand, within BT4324 at 5697.706 kb on - strand at 5697.706 kb on - strand at 5697.792 kb on + strand at 5697.792 kb on + strand at 5697.801 kb on - strand at 5697.860 kb on + strand at 5697.901 kb on + strand at 5697.961 kb on + strand at 5697.964 kb on + strand at 5697.994 kb on + strand at 5698.000 kb on + strand at 5698.061 kb on + strand, within BT4325 at 5698.062 kb on - strand, within BT4325 at 5698.065 kb on + strand, within BT4325 at 5698.066 kb on - strand, within BT4325 at 5698.085 kb on + strand, within BT4325 at 5698.136 kb on + strand, within BT4325 at 5698.245 kb on + strand, within BT4325 at 5698.292 kb on + strand, within BT4325 at 5698.311 kb on + strand, within BT4325 at 5698.312 kb on - strand, within BT4325 at 5698.495 kb on + strand, within BT4325 at 5698.533 kb on + strand, within BT4325 at 5698.640 kb on - strand, within BT4325 at 5698.681 kb on + strand, within BT4325 at 5698.682 kb on - strand, within BT4325 at 5698.961 kb on - strand, within BT4325 at 5699.160 kb on + strand at 5699.161 kb on - strand at 5699.161 kb on - strand at 5699.197 kb on + strand at 5699.584 kb on - strand, within BT4326 at 5699.593 kb on + strand, within BT4326 at 5699.643 kb on + strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.736 kb on - strand, within BT4326 at 5699.825 kb on - strand at 5699.825 kb on - strand at 5699.839 kb on - strand at 5699.934 kb on + strand at 5699.938 kb on + strand at 5699.939 kb on - strand at 5700.015 kb on + strand, within BT4327 at 5700.048 kb on - strand, within BT4327
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 5,696,936 - BT4324 0.64 -2.5 5,696,936 - BT4324 0.64 +0.1 5,696,938 + BT4324 0.64 -3.2 5,696,966 + BT4324 0.65 +0.3 5,696,979 - BT4324 0.65 -0.8 5,697,306 - BT4324 0.78 -0.1 5,697,321 + BT4324 0.78 -1.2 5,697,389 - BT4324 0.81 -2.7 5,697,389 - BT4324 0.81 -0.6 5,697,389 - BT4324 0.81 +0.7 5,697,434 + BT4324 0.82 +0.5 5,697,435 - BT4324 0.82 -0.2 5,697,453 + BT4324 0.83 -3.9 5,697,706 - -1.0 5,697,706 - +1.3 5,697,792 + -0.2 5,697,792 + +0.9 5,697,801 - -1.0 5,697,860 + -2.0 5,697,901 + -2.7 5,697,961 + +1.4 5,697,964 + +0.4 5,697,994 + -0.7 5,698,000 + -0.6 5,698,061 + BT4325 0.12 -1.2 5,698,062 - BT4325 0.13 -2.0 5,698,065 + BT4325 0.13 -3.2 5,698,066 - BT4325 0.13 -2.7 5,698,085 + BT4325 0.14 -0.2 5,698,136 + BT4325 0.19 +0.4 5,698,245 + BT4325 0.28 -3.2 5,698,292 + BT4325 0.32 +0.8 5,698,311 + BT4325 0.33 -1.4 5,698,312 - BT4325 0.33 +0.8 5,698,495 + BT4325 0.48 -2.3 5,698,533 + BT4325 0.52 +0.8 5,698,640 - BT4325 0.60 +1.4 5,698,681 + BT4325 0.64 -0.6 5,698,682 - BT4325 0.64 -1.7 5,698,961 - BT4325 0.87 -2.3 5,699,160 + -1.2 5,699,161 - +0.6 5,699,161 - -1.3 5,699,197 + +0.3 5,699,584 - BT4326 0.65 +1.4 5,699,593 + BT4326 0.66 +0.4 5,699,643 + BT4326 0.74 +0.9 5,699,736 - BT4326 0.88 -1.9 5,699,736 - BT4326 0.88 -1.4 5,699,736 - BT4326 0.88 -2.6 5,699,825 - -2.4 5,699,825 - -1.3 5,699,839 - -2.8 5,699,934 + +1.4 5,699,938 + -2.4 5,699,939 - -0.4 5,700,015 + BT4327 0.12 -0.8 5,700,048 - BT4327 0.15 +0.9
Or see this region's nucleotide sequence