Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3728 and BT3729 are separated by 71 nucleotides BT3729 and BT3730 overlap by 4 nucleotides BT3730 and BT3731 are separated by 77 nucleotides BT3731 and BT3732 are separated by 97 nucleotides
BT3728: BT3728 - riboflavin biosynthesis protein ribD (NCBI ptt file), at 4,838,863 to 4,839,882
BT3728
BT3729: BT3729 - putative protoporphyrinogen oxidase (NCBI ptt file), at 4,839,954 to 4,840,790
BT3729
BT3730: BT3730 - putative regulatory protein (NCBI ptt file), at 4,840,787 to 4,841,269
BT3730
BT3731: BT3731 - orotate phosphoribosyltransferase (NCBI ptt file), at 4,841,347 to 4,841,985
BT3731
BT3732: BT3732 - hypothetical protein (NCBI ptt file), at 4,842,083 to 4,842,493
BT3732
Position (kb)
4840
4841
4842 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4839.919 kb on - strand at 4839.922 kb on - strand at 4840.075 kb on - strand, within BT3729 at 4840.295 kb on - strand, within BT3729 at 4840.295 kb on - strand, within BT3729 at 4840.298 kb on + strand, within BT3729 at 4840.300 kb on + strand, within BT3729 at 4840.300 kb on + strand, within BT3729 at 4840.301 kb on - strand, within BT3729 at 4840.373 kb on + strand, within BT3729 at 4840.395 kb on - strand, within BT3729 at 4840.485 kb on + strand, within BT3729 at 4840.493 kb on - strand, within BT3729 at 4840.687 kb on - strand, within BT3729 at 4840.702 kb on + strand, within BT3729 at 4840.896 kb on + strand, within BT3730 at 4840.987 kb on + strand, within BT3730 at 4841.023 kb on - strand, within BT3730 at 4841.991 kb on + strand at 4842.036 kb on + strand at 4842.048 kb on + strand at 4842.068 kb on + strand at 4842.082 kb on + strand at 4842.120 kb on - strand at 4842.120 kb on - strand at 4842.138 kb on - strand, within BT3732
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 4,839,919 - -0.8 4,839,922 - +0.0 4,840,075 - BT3729 0.14 +0.5 4,840,295 - BT3729 0.41 +1.4 4,840,295 - BT3729 0.41 +0.6 4,840,298 + BT3729 0.41 +1.5 4,840,300 + BT3729 0.41 +0.2 4,840,300 + BT3729 0.41 +0.3 4,840,301 - BT3729 0.41 -3.6 4,840,373 + BT3729 0.50 +0.1 4,840,395 - BT3729 0.53 -1.9 4,840,485 + BT3729 0.63 -3.3 4,840,493 - BT3729 0.64 -4.0 4,840,687 - BT3729 0.88 -2.0 4,840,702 + BT3729 0.89 -0.8 4,840,896 + BT3730 0.23 -1.8 4,840,987 + BT3730 0.41 +0.4 4,841,023 - BT3730 0.49 -3.6 4,841,991 + -1.2 4,842,036 + +0.4 4,842,048 + -1.2 4,842,068 + -0.6 4,842,082 + -2.5 4,842,120 - -0.0 4,842,120 - -0.6 4,842,138 - BT3732 0.13 -3.4
Or see this region's nucleotide sequence