Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2752 and BT2753 are separated by 201 nucleotides BT2753 and BT2754 are separated by 58 nucleotides BT2754 and BT2755 are separated by 227 nucleotides
BT2752: BT2752 - primosomal protein N' (replication factor Y) (NCBI ptt file), at 3,410,820 to 3,413,276
BT2752
BT2753: BT2753 - hypothetical protein (NCBI ptt file), at 3,413,478 to 3,414,068
BT2753
BT2754: BT2754 - conserved hypothetical protein (NCBI ptt file), at 3,414,127 to 3,415,107
BT2754
BT2755: BT2755 - aspartate ammonia-lyase (NCBI ptt file), at 3,415,335 to 3,416,795
BT2755
Position (kb)
3414
3415
3416 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3413.345 kb on + strand at 3413.372 kb on + strand at 3413.372 kb on + strand at 3413.468 kb on - strand at 3413.489 kb on - strand at 3413.570 kb on + strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.583 kb on + strand, within BT2753 at 3413.584 kb on - strand, within BT2753 at 3413.667 kb on + strand, within BT2753 at 3413.668 kb on - strand, within BT2753 at 3413.675 kb on + strand, within BT2753 at 3413.675 kb on + strand, within BT2753 at 3413.678 kb on + strand, within BT2753 at 3413.719 kb on + strand, within BT2753 at 3413.719 kb on + strand, within BT2753 at 3413.720 kb on - strand, within BT2753 at 3413.720 kb on - strand at 3413.753 kb on - strand, within BT2753 at 3413.879 kb on + strand, within BT2753 at 3413.963 kb on + strand, within BT2753 at 3413.973 kb on + strand, within BT2753 at 3413.973 kb on + strand, within BT2753 at 3413.985 kb on + strand, within BT2753 at 3414.029 kb on + strand at 3414.029 kb on + strand at 3414.029 kb on + strand at 3414.033 kb on + strand at 3414.034 kb on - strand at 3414.034 kb on - strand at 3414.034 kb on - strand at 3414.068 kb on + strand at 3414.074 kb on + strand at 3414.083 kb on - strand at 3414.101 kb on - strand at 3414.104 kb on + strand at 3414.105 kb on - strand at 3414.108 kb on + strand at 3414.128 kb on + strand at 3414.177 kb on + strand at 3414.177 kb on + strand at 3414.177 kb on + strand at 3414.210 kb on + strand at 3414.217 kb on - strand at 3414.304 kb on + strand, within BT2754 at 3414.316 kb on - strand, within BT2754 at 3414.330 kb on - strand, within BT2754 at 3414.442 kb on + strand, within BT2754 at 3414.579 kb on + strand, within BT2754 at 3414.647 kb on + strand, within BT2754 at 3414.647 kb on + strand, within BT2754 at 3414.650 kb on + strand, within BT2754 at 3414.650 kb on + strand, within BT2754 at 3414.726 kb on + strand, within BT2754 at 3414.727 kb on - strand, within BT2754 at 3414.828 kb on - strand, within BT2754 at 3414.864 kb on + strand, within BT2754 at 3414.865 kb on - strand, within BT2754 at 3414.892 kb on - strand, within BT2754 at 3414.977 kb on + strand, within BT2754 at 3415.009 kb on - strand, within BT2754 at 3415.033 kb on - strand at 3415.034 kb on + strand at 3415.035 kb on - strand at 3415.044 kb on + strand at 3415.044 kb on + strand at 3415.044 kb on + strand at 3415.049 kb on + strand at 3415.049 kb on + strand at 3415.050 kb on - strand at 3415.073 kb on - strand at 3415.074 kb on + strand at 3415.074 kb on + strand at 3415.075 kb on - strand at 3415.108 kb on - strand at 3415.152 kb on + strand at 3415.152 kb on + strand at 3415.152 kb on + strand at 3415.152 kb on + strand at 3415.152 kb on + strand at 3415.153 kb on - strand at 3415.173 kb on - strand at 3415.253 kb on - strand at 3415.265 kb on + strand at 3415.265 kb on + strand at 3415.270 kb on + strand at 3415.297 kb on + strand at 3415.297 kb on + strand at 3415.299 kb on + strand at 3415.300 kb on - strand at 3415.300 kb on - strand at 3415.330 kb on - strand at 3415.424 kb on + strand at 3415.479 kb on + strand at 3415.497 kb on - strand, within BT2755 at 3415.497 kb on - strand, within BT2755 at 3415.498 kb on + strand, within BT2755 at 3415.590 kb on - strand, within BT2755 at 3415.593 kb on + strand, within BT2755 at 3415.593 kb on + strand, within BT2755 at 3415.594 kb on - strand, within BT2755 at 3415.598 kb on + strand, within BT2755 at 3415.608 kb on - strand, within BT2755 at 3415.706 kb on + strand, within BT2755 at 3415.736 kb on + strand, within BT2755 at 3415.738 kb on + strand, within BT2755 at 3415.861 kb on - strand, within BT2755 at 3415.899 kb on + strand, within BT2755 at 3415.927 kb on - strand, within BT2755 at 3416.026 kb on + strand, within BT2755
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 3,413,345 + +0.4 3,413,372 + +0.0 3,413,372 + -2.3 3,413,468 - -3.1 3,413,489 - -2.5 3,413,570 + BT2753 0.16 -0.3 3,413,577 - BT2753 0.17 -2.0 3,413,577 - BT2753 0.17 +1.5 3,413,577 - BT2753 0.17 -0.8 3,413,577 - BT2753 0.17 +0.5 3,413,583 + BT2753 0.18 +1.5 3,413,584 - BT2753 0.18 -2.6 3,413,667 + BT2753 0.32 -0.9 3,413,668 - BT2753 0.32 +1.5 3,413,675 + BT2753 0.33 -0.9 3,413,675 + BT2753 0.33 -1.4 3,413,678 + BT2753 0.34 -1.2 3,413,719 + BT2753 0.41 -1.5 3,413,719 + BT2753 0.41 -2.1 3,413,720 - BT2753 0.41 -2.4 3,413,720 - +0.5 3,413,753 - BT2753 0.47 +1.5 3,413,879 + BT2753 0.68 -2.5 3,413,963 + BT2753 0.82 -0.3 3,413,973 + BT2753 0.84 -2.5 3,413,973 + BT2753 0.84 -0.9 3,413,985 + BT2753 0.86 -0.5 3,414,029 + -3.2 3,414,029 + +0.1 3,414,029 + -0.4 3,414,033 + -2.0 3,414,034 - -1.1 3,414,034 - -1.7 3,414,034 - +0.2 3,414,068 + +0.5 3,414,074 + -0.8 3,414,083 - +1.5 3,414,101 - -2.5 3,414,104 + -2.7 3,414,105 - -0.1 3,414,108 + -0.2 3,414,128 + -0.5 3,414,177 + -0.5 3,414,177 + -0.1 3,414,177 + -0.0 3,414,210 + -0.2 3,414,217 - +1.7 3,414,304 + BT2754 0.18 +1.2 3,414,316 - BT2754 0.19 -1.8 3,414,330 - BT2754 0.21 +0.7 3,414,442 + BT2754 0.32 -4.4 3,414,579 + BT2754 0.46 -0.1 3,414,647 + BT2754 0.53 +1.1 3,414,647 + BT2754 0.53 -0.1 3,414,650 + BT2754 0.53 -3.1 3,414,650 + BT2754 0.53 -1.6 3,414,726 + BT2754 0.61 -2.3 3,414,727 - BT2754 0.61 -4.4 3,414,828 - BT2754 0.71 -2.1 3,414,864 + BT2754 0.75 -0.9 3,414,865 - BT2754 0.75 -0.8 3,414,892 - BT2754 0.78 +0.4 3,414,977 + BT2754 0.87 -2.5 3,415,009 - BT2754 0.90 -0.7 3,415,033 - +0.4 3,415,034 + -0.8 3,415,035 - +0.5 3,415,044 + -0.1 3,415,044 + -0.5 3,415,044 + -2.5 3,415,049 + +0.6 3,415,049 + -1.8 3,415,050 - -1.8 3,415,073 - -1.4 3,415,074 + +0.2 3,415,074 + +0.5 3,415,075 - +0.4 3,415,108 - +0.7 3,415,152 + -0.6 3,415,152 + +0.8 3,415,152 + -2.7 3,415,152 + -1.7 3,415,152 + -1.3 3,415,153 - -3.3 3,415,173 - -0.4 3,415,253 - +1.3 3,415,265 + +0.9 3,415,265 + +0.3 3,415,270 + -2.6 3,415,297 + -1.8 3,415,297 + -3.2 3,415,299 + +0.7 3,415,300 - +0.6 3,415,300 - +0.5 3,415,330 - -2.6 3,415,424 + -0.5 3,415,479 + +1.5 3,415,497 - BT2755 0.11 -1.5 3,415,497 - BT2755 0.11 +0.5 3,415,498 + BT2755 0.11 -1.7 3,415,590 - BT2755 0.17 -1.9 3,415,593 + BT2755 0.18 +0.5 3,415,593 + BT2755 0.18 +0.0 3,415,594 - BT2755 0.18 +0.1 3,415,598 + BT2755 0.18 -0.7 3,415,608 - BT2755 0.19 +0.1 3,415,706 + BT2755 0.25 -2.3 3,415,736 + BT2755 0.27 +1.2 3,415,738 + BT2755 0.28 -0.8 3,415,861 - BT2755 0.36 +0.7 3,415,899 + BT2755 0.39 -2.7 3,415,927 - BT2755 0.41 -2.0 3,416,026 + BT2755 0.47 +0.7
Or see this region's nucleotide sequence