Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2411 and BT2412 overlap by 4 nucleotides BT2412 and BT2413 overlap by 4 nucleotides
BT2411: BT2411 - hypothetical protein (NCBI ptt file), at 3,011,355 to 3,012,389
BT2411
BT2412: BT2412 - putative collagenase (NCBI ptt file), at 3,012,386 to 3,014,215
BT2412
BT2413: BT2413 - homoserine O-succinyltransferase (NCBI ptt file), at 3,014,212 to 3,015,129
BT2413
Position (kb)
3012
3013
3014
3015 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3011.428 kb on + strand at 3011.433 kb on - strand at 3011.433 kb on - strand at 3011.433 kb on - strand at 3011.433 kb on - strand at 3011.433 kb on - strand at 3011.434 kb on + strand at 3011.435 kb on - strand at 3011.441 kb on + strand at 3011.504 kb on - strand, within BT2411 at 3011.507 kb on + strand, within BT2411 at 3011.508 kb on - strand, within BT2411 at 3011.508 kb on - strand, within BT2411 at 3011.508 kb on - strand, within BT2411 at 3011.711 kb on - strand, within BT2411 at 3011.772 kb on - strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.813 kb on + strand, within BT2411 at 3011.866 kb on + strand, within BT2411 at 3011.988 kb on + strand, within BT2411 at 3012.012 kb on - strand, within BT2411 at 3012.092 kb on + strand, within BT2411 at 3012.092 kb on + strand, within BT2411 at 3012.095 kb on - strand, within BT2411 at 3012.095 kb on - strand, within BT2411 at 3012.106 kb on + strand, within BT2411 at 3012.107 kb on - strand, within BT2411 at 3012.107 kb on - strand, within BT2411 at 3012.153 kb on + strand, within BT2411 at 3012.177 kb on - strand, within BT2411 at 3012.179 kb on - strand, within BT2411 at 3012.255 kb on - strand, within BT2411 at 3012.255 kb on - strand, within BT2411 at 3012.330 kb on - strand at 3012.330 kb on - strand at 3012.366 kb on - strand at 3012.469 kb on - strand at 3012.472 kb on - strand at 3012.472 kb on - strand at 3012.484 kb on - strand at 3012.526 kb on + strand at 3012.586 kb on - strand, within BT2412 at 3012.660 kb on + strand, within BT2412 at 3012.664 kb on + strand, within BT2412 at 3012.665 kb on - strand, within BT2412 at 3012.665 kb on - strand, within BT2412 at 3012.730 kb on + strand, within BT2412 at 3012.859 kb on + strand, within BT2412 at 3012.932 kb on - strand, within BT2412 at 3012.996 kb on - strand, within BT2412 at 3013.037 kb on + strand, within BT2412 at 3013.037 kb on + strand, within BT2412 at 3013.171 kb on + strand, within BT2412 at 3013.249 kb on + strand, within BT2412 at 3013.249 kb on + strand, within BT2412 at 3013.423 kb on + strand, within BT2412 at 3013.430 kb on + strand, within BT2412 at 3013.431 kb on - strand, within BT2412 at 3013.454 kb on + strand, within BT2412 at 3013.467 kb on + strand, within BT2412 at 3013.467 kb on + strand, within BT2412 at 3013.468 kb on - strand, within BT2412 at 3013.468 kb on - strand, within BT2412 at 3013.553 kb on - strand, within BT2412 at 3013.686 kb on + strand, within BT2412 at 3013.687 kb on - strand, within BT2412 at 3013.701 kb on + strand, within BT2412 at 3013.701 kb on + strand, within BT2412 at 3013.702 kb on - strand, within BT2412 at 3013.702 kb on - strand, within BT2412 at 3013.707 kb on - strand, within BT2412 at 3013.724 kb on + strand, within BT2412 at 3013.871 kb on - strand, within BT2412 at 3014.022 kb on + strand, within BT2412 at 3014.022 kb on + strand, within BT2412 at 3014.022 kb on + strand, within BT2412 at 3014.027 kb on - strand, within BT2412 at 3014.038 kb on - strand at 3014.038 kb on - strand at 3014.038 kb on - strand at 3014.054 kb on + strand at 3014.122 kb on + strand at 3014.122 kb on + strand at 3014.124 kb on + strand at 3014.125 kb on - strand at 3014.125 kb on - strand at 3014.126 kb on + strand at 3014.127 kb on - strand at 3014.127 kb on - strand at 3014.150 kb on - strand at 3014.221 kb on - strand at 3014.221 kb on - strand at 3014.226 kb on + strand at 3014.235 kb on - strand at 3014.253 kb on + strand at 3014.335 kb on + strand, within BT2413 at 3014.335 kb on + strand, within BT2413 at 3014.342 kb on + strand, within BT2413 at 3014.343 kb on - strand, within BT2413 at 3014.390 kb on + strand, within BT2413 at 3014.408 kb on + strand, within BT2413 at 3014.469 kb on + strand, within BT2413 at 3014.469 kb on + strand, within BT2413 at 3014.469 kb on + strand, within BT2413 at 3014.469 kb on + strand, within BT2413 at 3014.470 kb on - strand, within BT2413 at 3014.471 kb on + strand, within BT2413 at 3014.472 kb on - strand, within BT2413 at 3014.472 kb on - strand, within BT2413 at 3014.539 kb on + strand, within BT2413 at 3014.539 kb on + strand, within BT2413 at 3014.540 kb on - strand, within BT2413 at 3014.567 kb on + strand, within BT2413 at 3014.567 kb on + strand, within BT2413 at 3014.568 kb on - strand, within BT2413 at 3014.568 kb on - strand, within BT2413 at 3014.682 kb on - strand, within BT2413 at 3014.707 kb on + strand, within BT2413 at 3014.707 kb on + strand, within BT2413 at 3014.707 kb on + strand, within BT2413 at 3014.710 kb on - strand, within BT2413 at 3014.712 kb on - strand, within BT2413 at 3014.713 kb on + strand, within BT2413 at 3014.714 kb on - strand, within BT2413 at 3014.718 kb on + strand, within BT2413 at 3014.719 kb on - strand, within BT2413 at 3014.771 kb on + strand, within BT2413 at 3014.772 kb on - strand, within BT2413 at 3014.864 kb on + strand, within BT2413 at 3014.877 kb on + strand, within BT2413 at 3014.885 kb on - strand, within BT2413 at 3014.904 kb on + strand, within BT2413 at 3014.904 kb on + strand, within BT2413 at 3014.985 kb on + strand, within BT2413 at 3014.985 kb on + strand, within BT2413 at 3015.058 kb on - strand at 3015.130 kb on - strand at 3015.138 kb on + strand at 3015.138 kb on + strand at 3015.139 kb on - strand at 3015.140 kb on + strand at 3015.140 kb on + strand at 3015.140 kb on + strand at 3015.141 kb on - strand at 3015.141 kb on - strand at 3015.141 kb on - strand at 3015.141 kb on - strand at 3015.143 kb on + strand at 3015.181 kb on + strand at 3015.182 kb on - strand at 3015.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 3,011,428 + -0.8 3,011,433 - -1.0 3,011,433 - +0.4 3,011,433 - +4.2 3,011,433 - +1.4 3,011,433 - -0.6 3,011,434 + +1.0 3,011,435 - -0.6 3,011,441 + +1.1 3,011,504 - BT2411 0.14 +0.7 3,011,507 + BT2411 0.15 +0.4 3,011,508 - BT2411 0.15 +0.1 3,011,508 - BT2411 0.15 +0.8 3,011,508 - BT2411 0.15 +0.8 3,011,711 - BT2411 0.34 +1.5 3,011,772 - BT2411 0.40 -1.1 3,011,813 + BT2411 0.44 -2.4 3,011,813 + BT2411 0.44 -3.9 3,011,813 + BT2411 0.44 -1.2 3,011,813 + BT2411 0.44 +2.4 3,011,813 + BT2411 0.44 -1.0 3,011,813 + BT2411 0.44 +0.6 3,011,866 + BT2411 0.49 -2.2 3,011,988 + BT2411 0.61 -0.8 3,012,012 - BT2411 0.63 +0.8 3,012,092 + BT2411 0.71 -1.0 3,012,092 + BT2411 0.71 +1.0 3,012,095 - BT2411 0.71 +1.2 3,012,095 - BT2411 0.71 +3.4 3,012,106 + BT2411 0.73 +0.7 3,012,107 - BT2411 0.73 +1.5 3,012,107 - BT2411 0.73 +0.7 3,012,153 + BT2411 0.77 +0.5 3,012,177 - BT2411 0.79 +0.5 3,012,179 - BT2411 0.80 +1.9 3,012,255 - BT2411 0.87 +1.5 3,012,255 - BT2411 0.87 +0.8 3,012,330 - +5.1 3,012,330 - +1.6 3,012,366 - -3.2 3,012,469 - +0.2 3,012,472 - -3.9 3,012,472 - -3.8 3,012,484 - +1.4 3,012,526 + +1.0 3,012,586 - BT2412 0.11 -1.8 3,012,660 + BT2412 0.15 +0.3 3,012,664 + BT2412 0.15 -2.3 3,012,665 - BT2412 0.15 +0.2 3,012,665 - BT2412 0.15 +0.7 3,012,730 + BT2412 0.19 +0.8 3,012,859 + BT2412 0.26 -1.5 3,012,932 - BT2412 0.30 -2.8 3,012,996 - BT2412 0.33 +0.2 3,013,037 + BT2412 0.36 +0.6 3,013,037 + BT2412 0.36 -1.2 3,013,171 + BT2412 0.43 +1.8 3,013,249 + BT2412 0.47 -2.8 3,013,249 + BT2412 0.47 +1.4 3,013,423 + BT2412 0.57 +1.1 3,013,430 + BT2412 0.57 +0.9 3,013,431 - BT2412 0.57 +0.3 3,013,454 + BT2412 0.58 -2.0 3,013,467 + BT2412 0.59 +1.4 3,013,467 + BT2412 0.59 +2.7 3,013,468 - BT2412 0.59 +1.7 3,013,468 - BT2412 0.59 +1.4 3,013,553 - BT2412 0.64 -2.0 3,013,686 + BT2412 0.71 -1.6 3,013,687 - BT2412 0.71 +1.8 3,013,701 + BT2412 0.72 +0.4 3,013,701 + BT2412 0.72 +0.8 3,013,702 - BT2412 0.72 -0.7 3,013,702 - BT2412 0.72 +0.7 3,013,707 - BT2412 0.72 -2.7 3,013,724 + BT2412 0.73 +1.4 3,013,871 - BT2412 0.81 +0.7 3,014,022 + BT2412 0.89 +1.1 3,014,022 + BT2412 0.89 -1.5 3,014,022 + BT2412 0.89 +1.6 3,014,027 - BT2412 0.90 +0.4 3,014,038 - -3.0 3,014,038 - -1.2 3,014,038 - +0.8 3,014,054 + -0.7 3,014,122 + +2.4 3,014,122 + +1.5 3,014,124 + -1.8 3,014,125 - +0.6 3,014,125 - -0.8 3,014,126 + +2.9 3,014,127 - -0.7 3,014,127 - +0.4 3,014,150 - -0.6 3,014,221 - -3.0 3,014,221 - -0.9 3,014,226 + -1.8 3,014,235 - -2.0 3,014,253 + +1.2 3,014,335 + BT2413 0.13 +1.6 3,014,335 + BT2413 0.13 +1.1 3,014,342 + BT2413 0.14 +1.5 3,014,343 - BT2413 0.14 -0.5 3,014,390 + BT2413 0.19 +1.5 3,014,408 + BT2413 0.21 -2.0 3,014,469 + BT2413 0.28 +3.4 3,014,469 + BT2413 0.28 +0.5 3,014,469 + BT2413 0.28 -1.7 3,014,469 + BT2413 0.28 -1.6 3,014,470 - BT2413 0.28 +1.7 3,014,471 + BT2413 0.28 -2.8 3,014,472 - BT2413 0.28 -1.1 3,014,472 - BT2413 0.28 +0.5 3,014,539 + BT2413 0.36 +2.4 3,014,539 + BT2413 0.36 -2.3 3,014,540 - BT2413 0.36 -2.4 3,014,567 + BT2413 0.39 -2.0 3,014,567 + BT2413 0.39 +1.9 3,014,568 - BT2413 0.39 -0.4 3,014,568 - BT2413 0.39 -2.6 3,014,682 - BT2413 0.51 +0.2 3,014,707 + BT2413 0.54 +0.6 3,014,707 + BT2413 0.54 +2.7 3,014,707 + BT2413 0.54 -0.9 3,014,710 - BT2413 0.54 -0.3 3,014,712 - BT2413 0.54 -1.4 3,014,713 + BT2413 0.55 +1.6 3,014,714 - BT2413 0.55 -2.4 3,014,718 + BT2413 0.55 +2.5 3,014,719 - BT2413 0.55 -0.3 3,014,771 + BT2413 0.61 -2.9 3,014,772 - BT2413 0.61 -0.9 3,014,864 + BT2413 0.71 +2.9 3,014,877 + BT2413 0.72 +0.6 3,014,885 - BT2413 0.73 +1.4 3,014,904 + BT2413 0.75 +1.2 3,014,904 + BT2413 0.75 -2.3 3,014,985 + BT2413 0.84 +1.0 3,014,985 + BT2413 0.84 -0.2 3,015,058 - -1.9 3,015,130 - -0.8 3,015,138 + +1.4 3,015,138 + -1.6 3,015,139 - -0.7 3,015,140 + -1.0 3,015,140 + +1.7 3,015,140 + -2.6 3,015,141 - -0.9 3,015,141 - +2.4 3,015,141 - +1.4 3,015,141 - -0.6 3,015,143 + -1.5 3,015,181 + -0.9 3,015,182 - +0.1 3,015,197 + -0.6
Or see this region's nucleotide sequence