Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2140 and BT2141 are separated by 95 nucleotides BT2141 and BT2142 are separated by 8 nucleotides
BT2140: BT2140 - putative sodium-dependent transporter (NCBI ptt file), at 2,693,952 to 2,694,845
BT2140
BT2141: BT2141 - putative N-acetylmuramoyl-L-alanine amidase amiA (NCBI ptt file), at 2,694,941 to 2,696,203
BT2141
BT2142: BT2142 - conserved hypothetical protein, with a conserved domain (NCBI ptt file), at 2,696,212 to 2,697,105
BT2142
Position (kb)
2694
2695
2696
2697 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2693.944 kb on + strand at 2694.059 kb on + strand, within BT2140 at 2694.060 kb on - strand, within BT2140 at 2694.060 kb on - strand, within BT2140 at 2694.087 kb on - strand, within BT2140 at 2694.130 kb on + strand, within BT2140 at 2694.286 kb on + strand, within BT2140 at 2694.312 kb on + strand, within BT2140 at 2694.427 kb on - strand, within BT2140 at 2694.445 kb on - strand, within BT2140 at 2694.474 kb on - strand, within BT2140 at 2694.485 kb on + strand, within BT2140 at 2694.501 kb on - strand, within BT2140 at 2694.502 kb on + strand, within BT2140 at 2694.502 kb on + strand, within BT2140 at 2694.503 kb on - strand, within BT2140 at 2694.503 kb on - strand, within BT2140 at 2694.566 kb on + strand, within BT2140 at 2694.576 kb on - strand, within BT2140 at 2694.576 kb on - strand, within BT2140 at 2694.579 kb on - strand, within BT2140 at 2694.582 kb on + strand, within BT2140 at 2694.582 kb on + strand, within BT2140 at 2694.583 kb on - strand, within BT2140 at 2694.583 kb on - strand, within BT2140 at 2694.583 kb on - strand, within BT2140 at 2694.583 kb on - strand, within BT2140 at 2694.583 kb on - strand, within BT2140 at 2694.620 kb on - strand, within BT2140 at 2694.631 kb on + strand, within BT2140 at 2694.632 kb on - strand, within BT2140 at 2694.632 kb on - strand, within BT2140 at 2694.687 kb on - strand, within BT2140 at 2694.743 kb on + strand, within BT2140 at 2694.743 kb on + strand, within BT2140 at 2694.774 kb on - strand at 2694.774 kb on - strand at 2694.813 kb on - strand at 2694.907 kb on - strand at 2694.981 kb on + strand at 2694.981 kb on + strand at 2695.004 kb on + strand at 2695.009 kb on - strand at 2695.013 kb on + strand at 2695.013 kb on + strand at 2695.224 kb on - strand, within BT2141 at 2695.224 kb on - strand, within BT2141 at 2695.226 kb on - strand, within BT2141 at 2695.427 kb on + strand, within BT2141 at 2695.451 kb on + strand, within BT2141 at 2695.484 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.514 kb on + strand, within BT2141 at 2695.730 kb on + strand, within BT2141 at 2695.749 kb on - strand, within BT2141 at 2695.858 kb on + strand, within BT2141 at 2695.859 kb on - strand, within BT2141 at 2695.901 kb on - strand, within BT2141 at 2695.901 kb on - strand, within BT2141 at 2695.943 kb on - strand, within BT2141 at 2696.022 kb on - strand, within BT2141 at 2696.022 kb on - strand, within BT2141 at 2696.045 kb on + strand, within BT2141 at 2696.047 kb on + strand, within BT2141 at 2696.069 kb on + strand, within BT2141 at 2696.082 kb on + strand at 2696.197 kb on + strand at 2696.308 kb on - strand, within BT2142 at 2696.433 kb on - strand, within BT2142 at 2696.703 kb on - strand, within BT2142 at 2696.996 kb on - strand, within BT2142 at 2697.132 kb on + strand at 2697.161 kb on + strand at 2697.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 2,693,944 + -0.3 2,694,059 + BT2140 0.12 +0.5 2,694,060 - BT2140 0.12 -1.8 2,694,060 - BT2140 0.12 +1.8 2,694,087 - BT2140 0.15 -0.7 2,694,130 + BT2140 0.20 +0.4 2,694,286 + BT2140 0.37 -0.2 2,694,312 + BT2140 0.40 +0.4 2,694,427 - BT2140 0.53 -0.4 2,694,445 - BT2140 0.55 -2.8 2,694,474 - BT2140 0.58 -2.7 2,694,485 + BT2140 0.60 +1.0 2,694,501 - BT2140 0.61 -1.8 2,694,502 + BT2140 0.62 +0.7 2,694,502 + BT2140 0.62 -1.2 2,694,503 - BT2140 0.62 +0.7 2,694,503 - BT2140 0.62 -1.9 2,694,566 + BT2140 0.69 -0.9 2,694,576 - BT2140 0.70 -3.2 2,694,576 - BT2140 0.70 -2.0 2,694,579 - BT2140 0.70 +0.1 2,694,582 + BT2140 0.70 +0.6 2,694,582 + BT2140 0.70 -2.6 2,694,583 - BT2140 0.71 +0.2 2,694,583 - BT2140 0.71 +0.9 2,694,583 - BT2140 0.71 -0.7 2,694,583 - BT2140 0.71 -3.1 2,694,583 - BT2140 0.71 +1.4 2,694,620 - BT2140 0.75 +0.9 2,694,631 + BT2140 0.76 +2.9 2,694,632 - BT2140 0.76 -0.6 2,694,632 - BT2140 0.76 -0.5 2,694,687 - BT2140 0.82 -1.6 2,694,743 + BT2140 0.88 +1.4 2,694,743 + BT2140 0.88 +1.4 2,694,774 - +0.5 2,694,774 - -3.0 2,694,813 - +0.3 2,694,907 - -3.0 2,694,981 + -2.2 2,694,981 + -1.4 2,695,004 + -1.4 2,695,009 - -0.6 2,695,013 + -3.0 2,695,013 + +1.6 2,695,224 - BT2141 0.22 -1.8 2,695,224 - BT2141 0.22 -2.8 2,695,226 - BT2141 0.23 -2.3 2,695,427 + BT2141 0.38 +1.4 2,695,451 + BT2141 0.40 -3.0 2,695,484 + BT2141 0.43 -0.6 2,695,514 + BT2141 0.45 +0.6 2,695,514 + BT2141 0.45 -2.6 2,695,514 + BT2141 0.45 -3.2 2,695,730 + BT2141 0.62 -2.6 2,695,749 - BT2141 0.64 -1.0 2,695,858 + BT2141 0.73 -1.1 2,695,859 - BT2141 0.73 +1.4 2,695,901 - BT2141 0.76 -2.0 2,695,901 - BT2141 0.76 -2.4 2,695,943 - BT2141 0.79 -2.2 2,696,022 - BT2141 0.86 -1.0 2,696,022 - BT2141 0.86 -3.3 2,696,045 + BT2141 0.87 -1.2 2,696,047 + BT2141 0.88 -0.3 2,696,069 + BT2141 0.89 -1.9 2,696,082 + -2.3 2,696,197 + -2.7 2,696,308 - BT2142 0.11 -2.2 2,696,433 - BT2142 0.25 -1.6 2,696,703 - BT2142 0.55 -2.2 2,696,996 - BT2142 0.88 -1.8 2,697,132 + -2.0 2,697,161 + -0.5 2,697,184 + +1.1
Or see this region's nucleotide sequence