Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1843 and BT1844 overlap by 4 nucleotides BT1844 and BT1845 are separated by 160 nucleotides BT1845 and BT1846 are separated by 8 nucleotides
BT1843: BT1843 - adenylosuccinate synthetase (NCBI ptt file), at 2,314,782 to 2,316,053
BT1843
BT1844: BT1844 - transcriptional regulator, putative iron uptake regulation protein (NCBI ptt file), at 2,316,050 to 2,316,541
BT1844
BT1845: BT1845 - conserved hypothetical protein (NCBI ptt file), at 2,316,702 to 2,317,367
BT1845
BT1846: BT1846 - putative dipeptidyl-peptidase III (NCBI ptt file), at 2,317,376 to 2,319,403
BT1846
Position (kb)
2316
2317
2318 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2315.705 kb on - strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.901 kb on + strand, within BT1843 at 2315.905 kb on + strand, within BT1843 at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.930 kb on + strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.931 kb on - strand at 2315.974 kb on + strand at 2315.985 kb on + strand at 2315.989 kb on - strand at 2316.034 kb on - strand at 2316.046 kb on - strand at 2316.092 kb on - strand at 2316.127 kb on + strand, within BT1844 at 2316.128 kb on - strand, within BT1844 at 2316.130 kb on - strand, within BT1844 at 2316.229 kb on + strand, within BT1844 at 2316.233 kb on + strand, within BT1844 at 2316.233 kb on + strand at 2316.233 kb on + strand, within BT1844 at 2316.274 kb on - strand, within BT1844 at 2316.337 kb on + strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.341 kb on - strand, within BT1844 at 2316.394 kb on - strand, within BT1844 at 2316.400 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.429 kb on + strand, within BT1844 at 2316.490 kb on - strand, within BT1844 at 2316.514 kb on + strand at 2316.568 kb on + strand at 2316.607 kb on + strand at 2316.612 kb on - strand at 2316.618 kb on + strand at 2316.619 kb on - strand at 2316.625 kb on + strand at 2316.628 kb on + strand at 2316.632 kb on + strand at 2316.633 kb on - strand at 2316.642 kb on - strand at 2316.642 kb on - strand at 2316.645 kb on + strand at 2316.682 kb on + strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.683 kb on - strand at 2316.687 kb on - strand at 2316.693 kb on + strand at 2316.697 kb on - strand at 2316.697 kb on - strand at 2316.700 kb on + strand at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.825 kb on - strand, within BT1845 at 2316.826 kb on + strand, within BT1845 at 2316.827 kb on - strand, within BT1845 at 2317.103 kb on + strand, within BT1845 at 2317.360 kb on - strand at 2317.382 kb on - strand at 2317.405 kb on - strand at 2317.437 kb on + strand at 2317.447 kb on + strand at 2317.501 kb on - strand at 2317.525 kb on - strand at 2317.549 kb on - strand at 2317.882 kb on - strand, within BT1846 at 2317.892 kb on - strand, within BT1846 at 2317.892 kb on - strand, within BT1846 at 2317.893 kb on + strand, within BT1846 at 2318.032 kb on - strand, within BT1846 at 2318.170 kb on + strand, within BT1846 at 2318.174 kb on - strand, within BT1846 at 2318.175 kb on + strand, within BT1846 at 2318.176 kb on - strand, within BT1846 at 2318.201 kb on - strand, within BT1846 at 2318.201 kb on - strand, within BT1846 at 2318.237 kb on - strand, within BT1846 at 2318.319 kb on - strand, within BT1846 at 2318.327 kb on + strand, within BT1846 at 2318.328 kb on - strand, within BT1846
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 2,315,705 - BT1843 0.73 +0.2 2,315,901 + BT1843 0.88 -1.4 2,315,901 + BT1843 0.88 +0.1 2,315,901 + BT1843 0.88 +0.5 2,315,905 + BT1843 0.88 +0.2 2,315,930 + +1.3 2,315,930 + -0.6 2,315,930 + +0.4 2,315,931 - -1.0 2,315,931 - +0.1 2,315,931 - -0.9 2,315,974 + -2.3 2,315,985 + -0.4 2,315,989 - -0.3 2,316,034 - +1.7 2,316,046 - +0.6 2,316,092 - +2.1 2,316,127 + BT1844 0.16 +0.3 2,316,128 - BT1844 0.16 -0.2 2,316,130 - BT1844 0.16 -0.2 2,316,229 + BT1844 0.36 +0.3 2,316,233 + BT1844 0.37 -5.8 2,316,233 + +1.3 2,316,233 + BT1844 0.37 +1.9 2,316,274 - BT1844 0.46 -1.5 2,316,337 + BT1844 0.58 -1.4 2,316,341 - BT1844 0.59 -0.3 2,316,341 - BT1844 0.59 -1.6 2,316,394 - BT1844 0.70 -2.8 2,316,400 + BT1844 0.71 +2.3 2,316,429 + BT1844 0.77 -0.5 2,316,429 + BT1844 0.77 -0.1 2,316,490 - BT1844 0.89 +0.3 2,316,514 + -2.7 2,316,568 + +2.4 2,316,607 + +1.3 2,316,612 - +0.8 2,316,618 + +0.2 2,316,619 - -0.7 2,316,625 + +2.3 2,316,628 + -3.9 2,316,632 + +1.5 2,316,633 - -2.2 2,316,642 - -1.2 2,316,642 - -1.3 2,316,645 + -2.4 2,316,682 + -0.3 2,316,683 - -2.6 2,316,683 - -2.2 2,316,683 - +1.3 2,316,687 - +0.9 2,316,693 + +0.7 2,316,697 - -0.1 2,316,697 - -0.3 2,316,700 + -0.6 2,316,825 - BT1845 0.18 -1.9 2,316,825 - BT1845 0.18 -1.9 2,316,825 - BT1845 0.18 +0.3 2,316,826 + BT1845 0.19 -2.2 2,316,827 - BT1845 0.19 -0.7 2,317,103 + BT1845 0.60 -1.7 2,317,360 - -1.7 2,317,382 - +2.3 2,317,405 - -1.3 2,317,437 + +0.3 2,317,447 + -0.3 2,317,501 - -2.2 2,317,525 - -1.7 2,317,549 - -3.2 2,317,882 - BT1846 0.25 -0.7 2,317,892 - BT1846 0.25 -0.3 2,317,892 - BT1846 0.25 -0.7 2,317,893 + BT1846 0.25 -0.3 2,318,032 - BT1846 0.32 -0.9 2,318,170 + BT1846 0.39 +1.5 2,318,174 - BT1846 0.39 -1.0 2,318,175 + BT1846 0.39 -2.0 2,318,176 - BT1846 0.39 +0.9 2,318,201 - BT1846 0.41 -1.9 2,318,201 - BT1846 0.41 -0.9 2,318,237 - BT1846 0.42 -0.4 2,318,319 - BT1846 0.46 -2.2 2,318,327 + BT1846 0.47 -0.3 2,318,328 - BT1846 0.47 +0.0
Or see this region's nucleotide sequence