Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1186 and tRNA-Lys are separated by 122 nucleotides tRNA-Lys and BT1187 are separated by 332 nucleotides BT1187 and BT1188 are separated by 262 nucleotides BT1188 and BT1189 are separated by 20 nucleotides
BT1186: BT1186 - ABC transporter ATP-binding protein (NCBI ptt file), at 1,481,408 to 1,483,096
BT1186
BT_t20: tRNA-Lys - tRNA-Lys (RefSeq), at 1,483,219 to 1,483,291
tRNA-Lys
BT1187: BT1187 - transcriptional regulator (NCBI ptt file), at 1,483,624 to 1,484,181
BT1187
BT1188: BT1188 - putative transcription regulator (NCBI ptt file), at 1,484,444 to 1,484,755
BT1188
BT1189: BT1189 - conserved hypothetical protein (NCBI ptt file), at 1,484,776 to 1,485,246
BT1189
Position (kb)
1483
1484
1485 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1483.323 kb on - strand at 1483.344 kb on - strand at 1483.356 kb on + strand at 1483.357 kb on - strand at 1483.363 kb on - strand at 1483.374 kb on + strand at 1483.447 kb on + strand at 1483.480 kb on + strand at 1483.488 kb on - strand at 1483.700 kb on + strand, within BT1187 at 1483.700 kb on + strand, within BT1187 at 1483.778 kb on + strand, within BT1187 at 1483.826 kb on - strand, within BT1187 at 1483.834 kb on + strand, within BT1187 at 1483.941 kb on + strand, within BT1187 at 1483.958 kb on + strand, within BT1187 at 1483.962 kb on - strand, within BT1187 at 1484.087 kb on - strand, within BT1187 at 1484.140 kb on + strand at 1484.160 kb on - strand at 1484.203 kb on + strand at 1484.345 kb on - strand at 1484.491 kb on - strand, within BT1188 at 1484.588 kb on + strand, within BT1188 at 1484.648 kb on + strand, within BT1188 at 1484.649 kb on - strand, within BT1188 at 1484.682 kb on - strand, within BT1188 at 1484.683 kb on + strand, within BT1188 at 1484.684 kb on - strand, within BT1188 at 1484.734 kb on + strand at 1484.737 kb on - strand at 1484.737 kb on - strand at 1485.074 kb on + strand, within BT1189 at 1485.115 kb on + strand, within BT1189 at 1485.115 kb on + strand, within BT1189 at 1485.173 kb on + strand, within BT1189
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,483,323 - -2.1 1,483,344 - -0.9 1,483,356 + -0.1 1,483,357 - +2.5 1,483,363 - -2.0 1,483,374 + +1.0 1,483,447 + -2.4 1,483,480 + -2.1 1,483,488 - +0.8 1,483,700 + BT1187 0.14 -1.5 1,483,700 + BT1187 0.14 +2.7 1,483,778 + BT1187 0.28 -3.1 1,483,826 - BT1187 0.36 +1.5 1,483,834 + BT1187 0.38 +0.9 1,483,941 + BT1187 0.57 +3.0 1,483,958 + BT1187 0.60 -0.2 1,483,962 - BT1187 0.61 +0.1 1,484,087 - BT1187 0.83 +0.1 1,484,140 + -0.9 1,484,160 - +1.1 1,484,203 + +1.5 1,484,345 - +1.5 1,484,491 - BT1188 0.15 +0.4 1,484,588 + BT1188 0.46 -0.9 1,484,648 + BT1188 0.65 -1.3 1,484,649 - BT1188 0.66 -1.7 1,484,682 - BT1188 0.76 -0.3 1,484,683 + BT1188 0.77 -1.5 1,484,684 - BT1188 0.77 -1.9 1,484,734 + +0.4 1,484,737 - +0.5 1,484,737 - -0.7 1,485,074 + BT1189 0.63 +0.1 1,485,115 + BT1189 0.72 -1.2 1,485,115 + BT1189 0.72 -1.4 1,485,173 + BT1189 0.84 -0.1
Or see this region's nucleotide sequence