Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1165 and BT1166 are separated by 3 nucleotides BT1166 and BT1167 are separated by 19 nucleotides BT1167 and BT1168 are separated by 155 nucleotides BT1168 and BT1169 overlap by 62 nucleotides
BT1165: BT1165 - hypothetical protein (NCBI ptt file), at 1,453,338 to 1,454,705
BT1165
BT1166: BT1166 - putative glycosyltransferase (NCBI ptt file), at 1,454,709 to 1,455,608
BT1166
BT1167: BT1167 - conserved hypothetical protein (NCBI ptt file), at 1,455,628 to 1,456,815
BT1167
BT1168: BT1168 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 1,456,971 to 1,457,414
BT1168
BT1169: BT1169 - hypothetical protein (NCBI ptt file), at 1,457,353 to 1,458,240
BT1169
Position (kb)
1455
1456
1457 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1454.830 kb on + strand, within BT1166 at 1454.831 kb on - strand, within BT1166 at 1454.875 kb on + strand, within BT1166 at 1454.875 kb on + strand, within BT1166 at 1454.876 kb on - strand, within BT1166 at 1454.876 kb on - strand, within BT1166 at 1454.991 kb on + strand, within BT1166 at 1455.135 kb on - strand, within BT1166 at 1455.282 kb on + strand, within BT1166 at 1455.400 kb on + strand, within BT1166 at 1455.401 kb on - strand, within BT1166 at 1455.401 kb on - strand, within BT1166 at 1455.496 kb on + strand, within BT1166 at 1455.631 kb on + strand at 1455.631 kb on + strand at 1455.632 kb on - strand at 1455.640 kb on - strand at 1455.695 kb on - strand at 1455.765 kb on - strand, within BT1167 at 1455.778 kb on + strand, within BT1167 at 1455.779 kb on - strand, within BT1167 at 1455.779 kb on - strand, within BT1167 at 1455.943 kb on - strand, within BT1167 at 1455.964 kb on - strand, within BT1167 at 1456.004 kb on + strand, within BT1167 at 1456.005 kb on - strand, within BT1167 at 1456.005 kb on - strand, within BT1167 at 1456.005 kb on - strand, within BT1167 at 1456.122 kb on + strand, within BT1167 at 1456.314 kb on - strand, within BT1167 at 1456.423 kb on + strand, within BT1167 at 1456.529 kb on + strand, within BT1167 at 1456.530 kb on - strand, within BT1167 at 1456.581 kb on - strand, within BT1167 at 1456.583 kb on + strand, within BT1167 at 1456.616 kb on - strand, within BT1167 at 1456.617 kb on + strand, within BT1167 at 1456.618 kb on - strand, within BT1167 at 1456.749 kb on - strand at 1456.755 kb on + strand at 1457.137 kb on - strand, within BT1168 at 1457.194 kb on + strand, within BT1168 at 1457.202 kb on - strand, within BT1168 at 1457.290 kb on + strand, within BT1168 at 1457.291 kb on - strand, within BT1168 at 1457.313 kb on - strand, within BT1168 at 1457.561 kb on + strand, within BT1169 at 1457.573 kb on + strand, within BT1169
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,454,830 + BT1166 0.13 +1.4 1,454,831 - BT1166 0.14 +1.1 1,454,875 + BT1166 0.18 -0.9 1,454,875 + BT1166 0.18 +0.1 1,454,876 - BT1166 0.19 -0.2 1,454,876 - BT1166 0.19 -1.2 1,454,991 + BT1166 0.31 -2.8 1,455,135 - BT1166 0.47 +0.4 1,455,282 + BT1166 0.64 -1.8 1,455,400 + BT1166 0.77 -1.0 1,455,401 - BT1166 0.77 +1.4 1,455,401 - BT1166 0.77 +0.9 1,455,496 + BT1166 0.87 -2.5 1,455,631 + -2.4 1,455,631 + -0.5 1,455,632 - -1.8 1,455,640 - -1.9 1,455,695 - -1.6 1,455,765 - BT1167 0.12 -1.4 1,455,778 + BT1167 0.13 -0.9 1,455,779 - BT1167 0.13 -0.6 1,455,779 - BT1167 0.13 -2.9 1,455,943 - BT1167 0.27 +0.1 1,455,964 - BT1167 0.28 -0.5 1,456,004 + BT1167 0.32 -0.3 1,456,005 - BT1167 0.32 -2.7 1,456,005 - BT1167 0.32 -0.8 1,456,005 - BT1167 0.32 -3.4 1,456,122 + BT1167 0.42 -2.2 1,456,314 - BT1167 0.58 -2.6 1,456,423 + BT1167 0.67 -2.0 1,456,529 + BT1167 0.76 +0.4 1,456,530 - BT1167 0.76 -3.7 1,456,581 - BT1167 0.80 +1.5 1,456,583 + BT1167 0.80 +0.9 1,456,616 - BT1167 0.83 +0.7 1,456,617 + BT1167 0.83 -2.8 1,456,618 - BT1167 0.83 -1.0 1,456,749 - +2.0 1,456,755 + -1.4 1,457,137 - BT1168 0.37 -1.8 1,457,194 + BT1168 0.50 +1.1 1,457,202 - BT1168 0.52 -1.6 1,457,290 + BT1168 0.72 -1.2 1,457,291 - BT1168 0.72 +1.0 1,457,313 - BT1168 0.77 +1.4 1,457,561 + BT1169 0.23 -0.9 1,457,573 + BT1169 0.25 -1.0
Or see this region's nucleotide sequence