Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1044 and BT1045 are separated by 9 nucleotides BT1045 and BT1046 are separated by 189 nucleotides
BT1044: BT1044 - hypothetical protein (NCBI ptt file), at 1,322,591 to 1,323,685
BT1044
BT1045: BT1045 - hypothetical protein (NCBI ptt file), at 1,323,695 to 1,324,885
BT1045
BT1046: BT1046 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 1,325,075 to 1,327,897
BT1046
Position (kb)
1323
1324
1325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1322.718 kb on + strand, within BT1044 at 1322.719 kb on - strand, within BT1044 at 1322.763 kb on - strand, within BT1044 at 1322.786 kb on - strand, within BT1044 at 1322.979 kb on + strand, within BT1044 at 1322.982 kb on + strand, within BT1044 at 1322.982 kb on + strand, within BT1044 at 1323.195 kb on - strand, within BT1044 at 1323.282 kb on + strand, within BT1044 at 1323.484 kb on + strand, within BT1044 at 1323.511 kb on + strand, within BT1044 at 1323.570 kb on + strand, within BT1044 at 1323.571 kb on - strand, within BT1044 at 1323.579 kb on + strand at 1323.580 kb on - strand at 1323.580 kb on - strand at 1323.583 kb on + strand at 1323.584 kb on - strand at 1323.587 kb on + strand at 1323.587 kb on + strand at 1323.587 kb on + strand at 1323.700 kb on + strand at 1323.719 kb on + strand at 1323.720 kb on - strand at 1323.742 kb on - strand at 1323.806 kb on + strand at 1323.806 kb on + strand at 1323.806 kb on + strand at 1323.807 kb on - strand at 1323.809 kb on - strand at 1323.814 kb on + strand, within BT1045 at 1323.817 kb on + strand, within BT1045 at 1323.953 kb on + strand, within BT1045 at 1323.954 kb on - strand, within BT1045 at 1323.956 kb on - strand, within BT1045 at 1323.973 kb on + strand, within BT1045 at 1323.977 kb on + strand, within BT1045 at 1323.977 kb on + strand, within BT1045 at 1323.979 kb on + strand, within BT1045 at 1323.979 kb on + strand, within BT1045 at 1323.984 kb on + strand, within BT1045 at 1323.992 kb on - strand, within BT1045 at 1324.057 kb on + strand, within BT1045 at 1324.086 kb on + strand, within BT1045 at 1324.115 kb on - strand, within BT1045 at 1324.161 kb on + strand, within BT1045 at 1324.161 kb on + strand, within BT1045 at 1324.186 kb on + strand, within BT1045 at 1324.252 kb on + strand, within BT1045 at 1324.364 kb on - strand, within BT1045 at 1324.395 kb on + strand, within BT1045 at 1324.395 kb on + strand, within BT1045 at 1324.399 kb on + strand, within BT1045 at 1324.488 kb on - strand, within BT1045 at 1324.490 kb on - strand, within BT1045 at 1324.490 kb on - strand, within BT1045 at 1324.506 kb on - strand, within BT1045 at 1324.665 kb on + strand, within BT1045 at 1324.676 kb on - strand, within BT1045 at 1324.719 kb on - strand, within BT1045 at 1324.798 kb on + strand at 1325.060 kb on + strand at 1325.089 kb on + strand at 1325.089 kb on + strand at 1325.089 kb on + strand at 1325.092 kb on + strand at 1325.303 kb on - strand at 1325.408 kb on + strand, within BT1046 at 1325.409 kb on - strand, within BT1046 at 1325.434 kb on + strand, within BT1046 at 1325.517 kb on + strand, within BT1046 at 1325.524 kb on + strand, within BT1046 at 1325.526 kb on + strand, within BT1046 at 1325.530 kb on + strand, within BT1046 at 1325.530 kb on + strand, within BT1046 at 1325.561 kb on + strand, within BT1046 at 1325.562 kb on - strand, within BT1046 at 1325.632 kb on + strand, within BT1046 at 1325.647 kb on + strand, within BT1046 at 1325.648 kb on - strand, within BT1046 at 1325.668 kb on + strand, within BT1046 at 1325.668 kb on + strand, within BT1046 at 1325.669 kb on - strand, within BT1046 at 1325.669 kb on - strand, within BT1046 at 1325.672 kb on - strand, within BT1046 at 1325.672 kb on - strand, within BT1046 at 1325.672 kb on - strand, within BT1046 at 1325.737 kb on + strand, within BT1046 at 1325.777 kb on - strand, within BT1046 at 1325.785 kb on - strand, within BT1046 at 1325.828 kb on + strand, within BT1046 at 1325.829 kb on - strand, within BT1046 at 1325.861 kb on - strand, within BT1046 at 1325.872 kb on + strand, within BT1046 at 1325.873 kb on - strand, within BT1046
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,322,718 + BT1044 0.12 +0.3 1,322,719 - BT1044 0.12 -1.0 1,322,763 - BT1044 0.16 -0.4 1,322,786 - BT1044 0.18 +0.0 1,322,979 + BT1044 0.35 -1.8 1,322,982 + BT1044 0.36 -1.5 1,322,982 + BT1044 0.36 -0.5 1,323,195 - BT1044 0.55 +1.0 1,323,282 + BT1044 0.63 -1.3 1,323,484 + BT1044 0.82 -1.2 1,323,511 + BT1044 0.84 -0.7 1,323,570 + BT1044 0.89 -1.7 1,323,571 - BT1044 0.89 +0.4 1,323,579 + -3.8 1,323,580 - +0.6 1,323,580 - -0.3 1,323,583 + +0.0 1,323,584 - -1.7 1,323,587 + -1.5 1,323,587 + +0.3 1,323,587 + -1.7 1,323,700 + -0.7 1,323,719 + -0.4 1,323,720 - -0.3 1,323,742 - -4.0 1,323,806 + -1.5 1,323,806 + -1.0 1,323,806 + +0.1 1,323,807 - -3.3 1,323,809 - -0.7 1,323,814 + BT1045 0.10 -1.0 1,323,817 + BT1045 0.10 -0.1 1,323,953 + BT1045 0.22 -1.3 1,323,954 - BT1045 0.22 -2.9 1,323,956 - BT1045 0.22 -2.4 1,323,973 + BT1045 0.23 -0.3 1,323,977 + BT1045 0.24 -2.1 1,323,977 + BT1045 0.24 -0.6 1,323,979 + BT1045 0.24 +0.7 1,323,979 + BT1045 0.24 +0.7 1,323,984 + BT1045 0.24 -2.4 1,323,992 - BT1045 0.25 -0.5 1,324,057 + BT1045 0.30 -2.9 1,324,086 + BT1045 0.33 -0.4 1,324,115 - BT1045 0.35 -0.6 1,324,161 + BT1045 0.39 -2.5 1,324,161 + BT1045 0.39 -0.5 1,324,186 + BT1045 0.41 +0.3 1,324,252 + BT1045 0.47 -2.7 1,324,364 - BT1045 0.56 -1.3 1,324,395 + BT1045 0.59 +1.7 1,324,395 + BT1045 0.59 -1.7 1,324,399 + BT1045 0.59 -1.3 1,324,488 - BT1045 0.67 +0.3 1,324,490 - BT1045 0.67 -2.8 1,324,490 - BT1045 0.67 +1.3 1,324,506 - BT1045 0.68 -1.4 1,324,665 + BT1045 0.81 -0.4 1,324,676 - BT1045 0.82 -0.7 1,324,719 - BT1045 0.86 -0.6 1,324,798 + +0.3 1,325,060 + -1.0 1,325,089 + -0.2 1,325,089 + +0.2 1,325,089 + -1.7 1,325,092 + -2.3 1,325,303 - -2.1 1,325,408 + BT1046 0.12 -1.5 1,325,409 - BT1046 0.12 -1.4 1,325,434 + BT1046 0.13 -1.7 1,325,517 + BT1046 0.16 -0.2 1,325,524 + BT1046 0.16 -1.7 1,325,526 + BT1046 0.16 -0.3 1,325,530 + BT1046 0.16 -2.5 1,325,530 + BT1046 0.16 -1.3 1,325,561 + BT1046 0.17 -2.3 1,325,562 - BT1046 0.17 -1.6 1,325,632 + BT1046 0.20 +0.8 1,325,647 + BT1046 0.20 -1.1 1,325,648 - BT1046 0.20 -3.4 1,325,668 + BT1046 0.21 +2.5 1,325,668 + BT1046 0.21 -1.5 1,325,669 - BT1046 0.21 -0.7 1,325,669 - BT1046 0.21 -1.0 1,325,672 - BT1046 0.21 -1.8 1,325,672 - BT1046 0.21 +0.8 1,325,672 - BT1046 0.21 -1.0 1,325,737 + BT1046 0.23 -2.0 1,325,777 - BT1046 0.25 -3.6 1,325,785 - BT1046 0.25 +2.5 1,325,828 + BT1046 0.27 -0.3 1,325,829 - BT1046 0.27 -0.4 1,325,861 - BT1046 0.28 +2.3 1,325,872 + BT1046 0.28 -2.9 1,325,873 - BT1046 0.28 -0.5
Or see this region's nucleotide sequence