Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0920 and BT0921 are separated by 200 nucleotides BT0921 and BT0922 are separated by 48 nucleotides BT0922 and BT0923 are separated by 23 nucleotides
BT0920: BT0920 - putative O-sialoglycoprotein endopeptidase (NCBI ptt file), at 1,130,452 to 1,131,471
BT0920
BT0921: BT0921 - conserved hypothetical protein (NCBI ptt file), at 1,131,672 to 1,136,126
BT0921
BT0922: BT0922 - hypothetical protein (NCBI ptt file), at 1,136,175 to 1,137,056
BT0922
BT0923: BT0923 - putative periplasmic protein (NCBI ptt file), at 1,137,080 to 1,137,517
BT0923
Position (kb)
1131
1132
1133
1134
1135
1136
1137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1131.485 kb on - strand at 1131.503 kb on - strand at 1131.593 kb on - strand at 1132.404 kb on - strand, within BT0921 at 1132.422 kb on - strand, within BT0921 at 1132.581 kb on + strand, within BT0921 at 1132.669 kb on + strand, within BT0921 at 1133.079 kb on - strand, within BT0921 at 1133.407 kb on - strand, within BT0921 at 1133.449 kb on - strand, within BT0921 at 1133.520 kb on - strand, within BT0921 at 1133.570 kb on - strand, within BT0921 at 1133.570 kb on - strand, within BT0921 at 1133.601 kb on - strand, within BT0921 at 1134.271 kb on - strand, within BT0921 at 1134.363 kb on + strand, within BT0921 at 1134.728 kb on + strand, within BT0921 at 1134.734 kb on + strand, within BT0921 at 1134.790 kb on - strand, within BT0921 at 1134.883 kb on - strand, within BT0921 at 1135.145 kb on - strand, within BT0921 at 1135.212 kb on + strand, within BT0921 at 1135.213 kb on - strand, within BT0921 at 1135.214 kb on + strand, within BT0921 at 1135.215 kb on - strand, within BT0921 at 1135.310 kb on + strand, within BT0921 at 1135.389 kb on - strand, within BT0921 at 1135.504 kb on - strand, within BT0921 at 1136.090 kb on + strand at 1136.144 kb on - strand at 1136.145 kb on + strand at 1136.150 kb on + strand at 1136.177 kb on - strand at 1136.207 kb on + strand at 1136.229 kb on - strand at 1136.255 kb on - strand at 1136.255 kb on - strand at 1136.884 kb on + strand, within BT0922 at 1136.885 kb on - strand, within BT0922 at 1136.885 kb on - strand, within BT0922 at 1136.885 kb on - strand, within BT0922 at 1136.929 kb on + strand, within BT0922 at 1137.033 kb on + strand at 1137.034 kb on - strand at 1137.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 1,131,485 - +0.5 1,131,503 - +2.4 1,131,593 - +3.5 1,132,404 - BT0921 0.16 +1.1 1,132,422 - BT0921 0.17 -0.7 1,132,581 + BT0921 0.20 +0.6 1,132,669 + BT0921 0.22 +3.1 1,133,079 - BT0921 0.32 -1.3 1,133,407 - BT0921 0.39 +1.5 1,133,449 - BT0921 0.40 +1.1 1,133,520 - BT0921 0.41 +0.4 1,133,570 - BT0921 0.43 -0.5 1,133,570 - BT0921 0.43 -2.8 1,133,601 - BT0921 0.43 +0.3 1,134,271 - BT0921 0.58 +1.9 1,134,363 + BT0921 0.60 +2.6 1,134,728 + BT0921 0.69 +2.3 1,134,734 + BT0921 0.69 +1.9 1,134,790 - BT0921 0.70 -0.3 1,134,883 - BT0921 0.72 -1.1 1,135,145 - BT0921 0.78 +1.8 1,135,212 + BT0921 0.79 -1.2 1,135,213 - BT0921 0.79 +2.3 1,135,214 + BT0921 0.80 +0.9 1,135,215 - BT0921 0.80 +2.1 1,135,310 + BT0921 0.82 -2.1 1,135,389 - BT0921 0.83 -1.6 1,135,504 - BT0921 0.86 -2.5 1,136,090 + +0.9 1,136,144 - -2.1 1,136,145 + -0.3 1,136,150 + -1.2 1,136,177 - -1.3 1,136,207 + +0.7 1,136,229 - -1.0 1,136,255 - +1.1 1,136,255 - +0.7 1,136,884 + BT0922 0.80 +0.9 1,136,885 - BT0922 0.80 -0.3 1,136,885 - BT0922 0.80 +0.9 1,136,885 - BT0922 0.80 -0.6 1,136,929 + BT0922 0.85 -1.1 1,137,033 + +0.5 1,137,034 - -2.0 1,137,086 - +0.7
Or see this region's nucleotide sequence