Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0432 and tRNA-Thr are separated by 66 nucleotides tRNA-Thr and BT0433 are separated by 129 nucleotides BT0433 and BT0434 are separated by 339 nucleotides
BT0432: BT0432 - putative endonuclease BB0411 (NCBI ptt file), at 519,272 to 520,150
BT0432
BT_t02: tRNA-Thr - tRNA-Thr (RefSeq), at 520,217 to 520,289
tRNA-Thr
BT0433: BT0433 - putative xylose repressor (NCBI ptt file), at 520,419 to 521,627
BT0433
BT0434: BT0434 - conserved hypothetical protein (NCBI ptt file), at 521,967 to 523,850
BT0434
Position (kb) 520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 519.426 kb on + strand, within BT0432 at 519.428 kb on + strand, within BT0432 at 519.649 kb on + strand, within BT0432 at 519.989 kb on + strand, within BT0432 at 520.003 kb on + strand, within BT0432 at 520.004 kb on - strand, within BT0432 at 520.020 kb on + strand, within BT0432 at 520.032 kb on + strand, within BT0432 at 520.039 kb on - strand, within BT0432 at 520.060 kb on + strand, within BT0432 at 520.081 kb on + strand at 520.172 kb on + strand at 520.246 kb on + strand, within tRNA-Thr at 520.329 kb on - strand at 521.181 kb on - strand, within BT0433 at 522.048 kb on - strand at 522.053 kb on - strand at 522.062 kb on + strand at 522.065 kb on + strand at 522.290 kb on - strand, within BT0434 at 522.522 kb on + strand, within BT0434 at 522.587 kb on + strand, within BT0434 at 522.603 kb on - strand, within BT0434 at 522.620 kb on - strand, within BT0434
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 519,426 + BT0432 0.18 -3.5 519,428 + BT0432 0.18 -0.6 519,649 + BT0432 0.43 -2.9 519,989 + BT0432 0.82 -1.4 520,003 + BT0432 0.83 +0.6 520,004 - BT0432 0.83 -0.3 520,020 + BT0432 0.85 -1.3 520,032 + BT0432 0.86 -2.6 520,039 - BT0432 0.87 +0.4 520,060 + BT0432 0.90 -1.2 520,081 + -2.2 520,172 + +0.1 520,246 + tRNA-Thr BT_t02 0.40 -0.9 520,329 - -0.5 521,181 - BT0433 0.63 +0.4 522,048 - -0.8 522,053 - -0.1 522,062 + -0.8 522,065 + -1.2 522,290 - BT0434 0.17 -1.5 522,522 + BT0434 0.29 +1.4 522,587 + BT0434 0.33 -0.6 522,603 - BT0434 0.34 -1.2 522,620 - BT0434 0.35 -0.4
Or see this region's nucleotide sequence