Experiment: BHIS with Dimethyl Sulfoxide 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_13 and p5482_14 are separated by 11 nucleotides p5482_14 and p5482_15 are separated by 4 nucleotides p5482_15 and p5482_16 overlap by 4 nucleotides
p5482_13: p5482_13 - hypothetical protein (NCBI), at 12,723 to 13,496
_13
p5482_14: p5482_14 - hypothetical protein (NCBI), at 13,508 to 14,152
_14
p5482_15: p5482_15 - TraM-like protein (NCBI), at 14,157 to 15,047
_15
p5482_16: p5482_16 - TraN-like protein (NCBI), at 15,044 to 16,141
_16
Position (kb) 14
15
16 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 13.157 kb on + strand, within p5482_13 at 13.158 kb on - strand, within p5482_13 at 13.158 kb on - strand, within p5482_13 at 13.163 kb on - strand, within p5482_13 at 13.182 kb on + strand, within p5482_13 at 13.218 kb on + strand, within p5482_13 at 13.219 kb on - strand, within p5482_13 at 13.224 kb on - strand, within p5482_13 at 13.224 kb on - strand, within p5482_13 at 13.224 kb on - strand, within p5482_13 at 13.226 kb on - strand, within p5482_13 at 13.226 kb on - strand, within p5482_13 at 13.256 kb on - strand, within p5482_13 at 13.256 kb on - strand, within p5482_13 at 13.256 kb on - strand, within p5482_13 at 13.324 kb on - strand, within p5482_13 at 13.324 kb on - strand, within p5482_13 at 13.349 kb on - strand, within p5482_13 at 13.368 kb on - strand, within p5482_13 at 13.368 kb on - strand, within p5482_13 at 13.368 kb on - strand, within p5482_13 at 13.368 kb on - strand, within p5482_13 at 13.383 kb on - strand, within p5482_13 at 13.383 kb on - strand, within p5482_13 at 13.383 kb on - strand, within p5482_13 at 13.383 kb on - strand, within p5482_13 at 13.383 kb on - strand, within p5482_13 at 13.411 kb on - strand, within p5482_13 at 13.411 kb on - strand, within p5482_13 at 13.411 kb on - strand, within p5482_13 at 13.411 kb on - strand, within p5482_13 at 13.411 kb on - strand, within p5482_13 at 13.413 kb on - strand, within p5482_13 at 13.413 kb on - strand, within p5482_13 at 13.413 kb on - strand, within p5482_13 at 13.507 kb on + strand at 13.507 kb on + strand at 13.508 kb on - strand at 13.508 kb on - strand at 13.508 kb on - strand at 13.514 kb on - strand at 13.514 kb on - strand at 13.522 kb on + strand at 13.523 kb on - strand at 13.523 kb on - strand at 13.523 kb on - strand at 13.523 kb on - strand at 13.524 kb on + strand at 13.525 kb on - strand at 13.529 kb on - strand at 13.541 kb on - strand at 13.541 kb on - strand at 13.775 kb on - strand, within p5482_14 at 13.866 kb on - strand, within p5482_14 at 13.868 kb on + strand, within p5482_14 at 13.869 kb on - strand, within p5482_14 at 13.870 kb on + strand, within p5482_14 at 13.871 kb on - strand, within p5482_14 at 13.871 kb on - strand, within p5482_14 at 13.916 kb on - strand, within p5482_14 at 13.916 kb on - strand, within p5482_14 at 13.916 kb on - strand, within p5482_14 at 13.927 kb on - strand, within p5482_14 at 13.927 kb on - strand, within p5482_14 at 13.964 kb on + strand, within p5482_14 at 13.965 kb on - strand, within p5482_14 at 13.965 kb on - strand, within p5482_14 at 14.108 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.128 kb on - strand at 14.151 kb on - strand at 14.157 kb on - strand at 14.161 kb on - strand at 14.295 kb on - strand, within p5482_15 at 14.295 kb on - strand, within p5482_15 at 14.331 kb on - strand, within p5482_15 at 14.382 kb on + strand, within p5482_15 at 14.403 kb on - strand, within p5482_15 at 14.406 kb on + strand, within p5482_15 at 14.407 kb on - strand, within p5482_15 at 14.429 kb on - strand, within p5482_15 at 14.446 kb on + strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.560 kb on + strand, within p5482_15 at 14.561 kb on - strand, within p5482_15 at 14.657 kb on - strand, within p5482_15 at 14.705 kb on + strand, within p5482_15 at 14.706 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.761 kb on + strand, within p5482_15 at 14.796 kb on + strand, within p5482_15 at 14.797 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.844 kb on + strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.916 kb on - strand, within p5482_15 at 14.923 kb on - strand, within p5482_15 at 14.939 kb on - strand, within p5482_15 at 14.943 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 15.028 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.034 kb on + strand at 15.034 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.053 kb on - strand at 15.059 kb on - strand at 15.124 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.151 kb on - strand at 15.159 kb on + strand, within p5482_16 at 15.159 kb on + strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.323 kb on - strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.401 kb on + strand, within p5482_16 at 15.452 kb on + strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.455 kb on - strand, within p5482_16 at 15.457 kb on - strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.649 kb on - strand, within p5482_16 at 15.738 kb on + strand, within p5482_16 at 15.739 kb on - strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.743 kb on - strand at 15.815 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.878 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.884 kb on + strand, within p5482_16 at 15.885 kb on - strand, within p5482_16 at 15.887 kb on - strand, within p5482_16 at 15.897 kb on - strand, within p5482_16 at 16.025 kb on - strand, within p5482_16
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 10 vol% remove 13,157 + p5482_13 0.56 -5.5 13,158 - p5482_13 0.56 +0.1 13,158 - p5482_13 0.56 -3.8 13,163 - p5482_13 0.57 +0.2 13,182 + p5482_13 0.59 -2.9 13,218 + p5482_13 0.64 -4.0 13,219 - p5482_13 0.64 +1.4 13,224 - p5482_13 0.65 +0.1 13,224 - p5482_13 0.65 +1.4 13,224 - p5482_13 0.65 -3.3 13,226 - p5482_13 0.65 +1.9 13,226 - p5482_13 0.65 +0.5 13,256 - p5482_13 0.69 +0.4 13,256 - p5482_13 0.69 +0.4 13,256 - p5482_13 0.69 +1.0 13,324 - p5482_13 0.78 -2.6 13,324 - p5482_13 0.78 -2.0 13,349 - p5482_13 0.81 -3.9 13,368 - p5482_13 0.83 +1.9 13,368 - p5482_13 0.83 +1.1 13,368 - p5482_13 0.83 -3.5 13,368 - p5482_13 0.83 -3.8 13,383 - p5482_13 0.85 +0.8 13,383 - p5482_13 0.85 -3.2 13,383 - p5482_13 0.85 +0.4 13,383 - p5482_13 0.85 -1.5 13,383 - p5482_13 0.85 -1.5 13,411 - p5482_13 0.89 -2.8 13,411 - p5482_13 0.89 +1.1 13,411 - p5482_13 0.89 -2.8 13,411 - p5482_13 0.89 +1.1 13,411 - p5482_13 0.89 +1.3 13,413 - p5482_13 0.89 -0.9 13,413 - p5482_13 0.89 -3.1 13,413 - p5482_13 0.89 -2.3 13,507 + -0.2 13,507 + -5.8 13,508 - +0.7 13,508 - -3.7 13,508 - -0.6 13,514 - -3.3 13,514 - +2.6 13,522 + -0.6 13,523 - -3.3 13,523 - +0.9 13,523 - +0.4 13,523 - +1.5 13,524 + -3.2 13,525 - +0.0 13,529 - +1.0 13,541 - +0.9 13,541 - -3.0 13,775 - p5482_14 0.41 -2.9 13,866 - p5482_14 0.56 -2.6 13,868 + p5482_14 0.56 -2.8 13,869 - p5482_14 0.56 +0.8 13,870 + p5482_14 0.56 +1.4 13,871 - p5482_14 0.56 +0.4 13,871 - p5482_14 0.56 -1.2 13,916 - p5482_14 0.63 -2.8 13,916 - p5482_14 0.63 -0.4 13,916 - p5482_14 0.63 -1.5 13,927 - p5482_14 0.65 -2.2 13,927 - p5482_14 0.65 -3.7 13,964 + p5482_14 0.71 -2.2 13,965 - p5482_14 0.71 +0.4 13,965 - p5482_14 0.71 +0.4 14,108 - +0.7 14,110 - -1.2 14,110 - +0.1 14,110 - -3.0 14,128 - -2.2 14,151 - +0.6 14,157 - -4.2 14,161 - +2.3 14,295 - p5482_15 0.15 -0.3 14,295 - p5482_15 0.15 -0.2 14,331 - p5482_15 0.20 -2.6 14,382 + p5482_15 0.25 -2.4 14,403 - p5482_15 0.28 -2.9 14,406 + p5482_15 0.28 -2.4 14,407 - p5482_15 0.28 -2.1 14,429 - p5482_15 0.31 -2.5 14,446 + p5482_15 0.32 -1.5 14,447 - p5482_15 0.33 +1.1 14,447 - p5482_15 0.33 -3.0 14,560 + p5482_15 0.45 -2.4 14,561 - p5482_15 0.45 -0.1 14,657 - p5482_15 0.56 +0.3 14,705 + p5482_15 0.62 -3.3 14,706 - p5482_15 0.62 +0.4 14,760 - p5482_15 0.68 +0.4 14,760 - p5482_15 0.68 -2.4 14,760 - p5482_15 0.68 +0.6 14,760 - p5482_15 0.68 +0.4 14,760 - p5482_15 0.68 +0.1 14,761 + p5482_15 0.68 -1.6 14,796 + p5482_15 0.72 -3.0 14,797 - p5482_15 0.72 -3.0 14,841 - p5482_15 0.77 +1.0 14,841 - p5482_15 0.77 +0.2 14,841 - p5482_15 0.77 +0.4 14,841 - p5482_15 0.77 +1.4 14,843 - p5482_15 0.77 +1.2 14,843 - p5482_15 0.77 -1.0 14,843 - p5482_15 0.77 +0.9 14,844 + p5482_15 0.77 -3.7 14,845 - p5482_15 0.77 +0.5 14,845 - p5482_15 0.77 +0.8 14,845 - p5482_15 0.77 +0.7 14,845 - p5482_15 0.77 +0.6 14,845 - p5482_15 0.77 +0.4 14,845 - p5482_15 0.77 +1.1 14,845 - p5482_15 0.77 +1.5 14,845 - p5482_15 0.77 -2.4 14,916 - p5482_15 0.85 -2.0 14,923 - p5482_15 0.86 +1.7 14,939 - p5482_15 0.88 +4.2 14,943 - p5482_15 0.88 -2.9 14,952 - p5482_15 0.89 -3.2 14,952 - p5482_15 0.89 +0.6 15,028 - +1.3 15,029 + -1.6 15,030 - +0.7 15,034 + +0.4 15,034 + -1.0 15,052 + +0.4 15,052 + -4.9 15,052 + -5.6 15,052 + -2.7 15,053 - +0.5 15,059 - +1.6 15,124 + -2.9 15,150 + +0.4 15,150 + -4.2 15,150 + -3.5 15,151 - -1.4 15,159 + p5482_16 0.10 -3.0 15,159 + p5482_16 0.10 -2.4 15,160 - p5482_16 0.11 +0.1 15,160 - p5482_16 0.11 +1.6 15,160 - p5482_16 0.11 -0.1 15,179 + p5482_16 0.12 -4.8 15,179 + p5482_16 0.12 -3.0 15,179 + p5482_16 0.12 -5.0 15,181 + p5482_16 0.12 -3.2 15,181 + p5482_16 0.12 -4.0 15,232 + p5482_16 0.17 +0.4 15,232 + p5482_16 0.17 +0.4 15,232 + p5482_16 0.17 -4.6 15,233 - p5482_16 0.17 +0.9 15,233 - p5482_16 0.17 -4.4 15,323 - p5482_16 0.25 +1.4 15,332 + p5482_16 0.26 -2.8 15,332 + p5482_16 0.26 -1.2 15,333 - p5482_16 0.26 +0.4 15,333 - p5482_16 0.26 -2.6 15,333 - p5482_16 0.26 +0.9 15,333 - p5482_16 0.26 -2.8 15,352 + p5482_16 0.28 -2.2 15,352 + p5482_16 0.28 -1.2 15,352 + p5482_16 0.28 -4.0 15,352 + p5482_16 0.28 -4.3 15,353 - p5482_16 0.28 -3.2 15,353 - p5482_16 0.28 +0.6 15,373 + p5482_16 0.30 -3.3 15,373 + p5482_16 0.30 -3.2 15,384 + p5482_16 0.31 -3.3 15,384 + p5482_16 0.31 -2.2 15,384 + p5482_16 0.31 -2.6 15,385 - p5482_16 0.31 -0.6 15,385 - p5482_16 0.31 +0.4 15,385 - p5482_16 0.31 -1.2 15,397 + p5482_16 0.32 -2.4 15,397 + p5482_16 0.32 -2.0 15,398 - p5482_16 0.32 +0.8 15,398 - p5482_16 0.32 +0.9 15,398 - p5482_16 0.32 +0.2 15,398 - p5482_16 0.32 -1.7 15,398 - p5482_16 0.32 -3.6 15,401 + p5482_16 0.33 -3.6 15,452 + p5482_16 0.37 -4.2 15,453 - p5482_16 0.37 +0.7 15,453 - p5482_16 0.37 -0.0 15,453 - p5482_16 0.37 -4.4 15,453 - p5482_16 0.37 +0.6 15,453 - p5482_16 0.37 +1.5 15,455 - p5482_16 0.37 -3.0 15,457 - p5482_16 0.38 +0.4 15,477 + p5482_16 0.39 -3.1 15,477 + p5482_16 0.39 -0.6 15,483 + p5482_16 0.40 -0.8 15,483 + p5482_16 0.40 -0.6 15,483 + p5482_16 0.40 -1.1 15,484 - p5482_16 0.40 +1.9 15,484 - p5482_16 0.40 -4.4 15,484 - p5482_16 0.40 -3.3 15,484 - p5482_16 0.40 +0.9 15,484 - p5482_16 0.40 -4.1 15,509 - p5482_16 0.42 +0.4 15,509 - p5482_16 0.42 -0.1 15,597 - p5482_16 0.50 -0.9 15,597 - p5482_16 0.50 +1.1 15,649 - p5482_16 0.55 -1.8 15,738 + p5482_16 0.63 +0.4 15,739 - p5482_16 0.63 +1.4 15,740 + p5482_16 0.63 -5.6 15,740 + p5482_16 0.63 -1.2 15,740 + p5482_16 0.63 -1.4 15,741 - p5482_16 0.63 -0.6 15,741 - p5482_16 0.63 -3.9 15,741 - p5482_16 0.63 +0.4 15,741 - p5482_16 0.63 -3.1 15,741 - p5482_16 0.63 +0.8 15,741 - p5482_16 0.63 -2.9 15,743 - -1.6 15,815 - p5482_16 0.70 +1.3 15,817 - p5482_16 0.70 -2.4 15,817 - p5482_16 0.70 -0.1 15,817 - p5482_16 0.70 -2.1 15,817 - p5482_16 0.70 -2.6 15,817 - p5482_16 0.70 -2.4 15,817 - p5482_16 0.70 -1.6 15,819 - p5482_16 0.71 +1.0 15,819 - p5482_16 0.71 +0.2 15,819 - p5482_16 0.71 +0.9 15,873 - p5482_16 0.76 +0.3 15,873 - p5482_16 0.76 -1.4 15,878 - p5482_16 0.76 -3.4 15,880 - p5482_16 0.76 +1.0 15,880 - p5482_16 0.76 -4.1 15,880 - p5482_16 0.76 +0.9 15,880 - p5482_16 0.76 +0.9 15,880 - p5482_16 0.76 +0.4 15,880 - p5482_16 0.76 -1.6 15,884 + p5482_16 0.77 -3.1 15,885 - p5482_16 0.77 -3.2 15,887 - p5482_16 0.77 -0.1 15,897 - p5482_16 0.78 +0.4 16,025 - p5482_16 0.89 +0.8
Or see this region's nucleotide sequence