Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4050 and BT4051 are separated by 120 nucleotides BT4051 and BT4052 are separated by 106 nucleotides
BT4050: BT4050 - beta-galactosidase (NCBI ptt file), at 5,274,028 to 5,278,056
BT4050
BT4051: BT4051 - conserved hypothetical protein (NCBI ptt file), at 5,278,177 to 5,279,094
BT4051
BT4052: BT4052 - putative ABC transporter ATP-binding protein (NCBI ptt file), at 5,279,201 to 5,281,090
BT4052
Position (kb)
5278
5279
5280 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5277.236 kb on - strand, within BT4050 at 5277.262 kb on + strand, within BT4050 at 5277.262 kb on + strand, within BT4050 at 5277.441 kb on + strand, within BT4050 at 5277.479 kb on - strand, within BT4050 at 5277.522 kb on + strand, within BT4050 at 5277.570 kb on + strand, within BT4050 at 5277.571 kb on - strand, within BT4050 at 5277.587 kb on - strand at 5277.588 kb on + strand, within BT4050 at 5277.589 kb on - strand, within BT4050 at 5277.589 kb on - strand, within BT4050 at 5277.594 kb on + strand, within BT4050 at 5277.666 kb on + strand at 5277.720 kb on + strand at 5277.739 kb on + strand at 5277.813 kb on + strand at 5277.818 kb on - strand at 5277.860 kb on - strand at 5277.860 kb on - strand at 5277.865 kb on - strand at 5277.909 kb on + strand at 5277.910 kb on - strand at 5277.977 kb on - strand at 5277.979 kb on - strand at 5278.054 kb on + strand at 5278.054 kb on + strand at 5278.070 kb on + strand at 5278.071 kb on - strand at 5278.071 kb on - strand at 5278.084 kb on - strand at 5278.084 kb on - strand at 5278.110 kb on - strand at 5278.233 kb on + strand at 5278.236 kb on - strand at 5278.258 kb on - strand at 5278.336 kb on - strand, within BT4051 at 5278.337 kb on + strand, within BT4051 at 5278.337 kb on + strand, within BT4051 at 5278.338 kb on - strand, within BT4051 at 5278.338 kb on - strand, within BT4051 at 5278.341 kb on + strand, within BT4051 at 5278.343 kb on + strand, within BT4051 at 5278.348 kb on - strand, within BT4051 at 5278.397 kb on + strand, within BT4051 at 5278.398 kb on - strand, within BT4051 at 5278.427 kb on + strand, within BT4051 at 5278.428 kb on - strand, within BT4051 at 5278.428 kb on - strand, within BT4051 at 5278.432 kb on + strand, within BT4051 at 5278.506 kb on - strand, within BT4051 at 5278.521 kb on - strand, within BT4051 at 5278.524 kb on + strand, within BT4051 at 5278.525 kb on - strand, within BT4051 at 5278.525 kb on - strand, within BT4051 at 5278.653 kb on + strand, within BT4051 at 5278.684 kb on - strand, within BT4051 at 5278.696 kb on - strand, within BT4051 at 5278.718 kb on - strand, within BT4051 at 5278.731 kb on + strand, within BT4051 at 5278.770 kb on + strand, within BT4051 at 5278.774 kb on + strand, within BT4051 at 5278.809 kb on + strand, within BT4051 at 5278.836 kb on + strand, within BT4051 at 5278.837 kb on - strand, within BT4051 at 5278.840 kb on + strand at 5278.960 kb on + strand, within BT4051 at 5279.106 kb on + strand at 5279.106 kb on + strand at 5279.113 kb on + strand at 5279.175 kb on - strand at 5279.187 kb on + strand at 5279.188 kb on - strand at 5279.219 kb on + strand at 5279.221 kb on + strand at 5279.264 kb on - strand at 5279.266 kb on - strand at 5279.266 kb on - strand at 5279.352 kb on - strand at 5279.450 kb on + strand, within BT4052 at 5279.450 kb on + strand, within BT4052 at 5279.451 kb on - strand, within BT4052 at 5279.451 kb on - strand, within BT4052 at 5279.558 kb on + strand, within BT4052 at 5279.559 kb on - strand, within BT4052 at 5279.606 kb on - strand, within BT4052 at 5279.697 kb on - strand, within BT4052 at 5279.730 kb on + strand, within BT4052 at 5279.752 kb on - strand, within BT4052 at 5279.752 kb on - strand, within BT4052 at 5279.760 kb on - strand, within BT4052 at 5279.786 kb on + strand, within BT4052 at 5280.086 kb on + strand, within BT4052
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 5,277,236 - BT4050 0.80 -0.4 5,277,262 + BT4050 0.80 +0.2 5,277,262 + BT4050 0.80 -2.9 5,277,441 + BT4050 0.85 +1.2 5,277,479 - BT4050 0.86 +0.6 5,277,522 + BT4050 0.87 -0.2 5,277,570 + BT4050 0.88 -0.0 5,277,571 - BT4050 0.88 +0.1 5,277,587 - +1.6 5,277,588 + BT4050 0.88 -2.0 5,277,589 - BT4050 0.88 +0.9 5,277,589 - BT4050 0.88 -1.9 5,277,594 + BT4050 0.89 -3.2 5,277,666 + +1.0 5,277,720 + -0.0 5,277,739 + -1.0 5,277,813 + +0.6 5,277,818 - -2.6 5,277,860 - -3.8 5,277,860 - -2.8 5,277,865 - +0.4 5,277,909 + +0.2 5,277,910 - -1.4 5,277,977 - -0.8 5,277,979 - +1.3 5,278,054 + +2.4 5,278,054 + -0.1 5,278,070 + +0.8 5,278,071 - -1.6 5,278,071 - +0.6 5,278,084 - -1.9 5,278,084 - +1.0 5,278,110 - -1.0 5,278,233 + -0.8 5,278,236 - -0.3 5,278,258 - +2.1 5,278,336 - BT4051 0.17 -0.5 5,278,337 + BT4051 0.17 -1.3 5,278,337 + BT4051 0.17 -0.3 5,278,338 - BT4051 0.18 -1.4 5,278,338 - BT4051 0.18 -1.7 5,278,341 + BT4051 0.18 +0.6 5,278,343 + BT4051 0.18 -0.5 5,278,348 - BT4051 0.19 -2.0 5,278,397 + BT4051 0.24 +1.6 5,278,398 - BT4051 0.24 +2.1 5,278,427 + BT4051 0.27 -0.0 5,278,428 - BT4051 0.27 -0.3 5,278,428 - BT4051 0.27 -0.4 5,278,432 + BT4051 0.28 +0.3 5,278,506 - BT4051 0.36 +0.4 5,278,521 - BT4051 0.37 -2.4 5,278,524 + BT4051 0.38 +2.1 5,278,525 - BT4051 0.38 -0.4 5,278,525 - BT4051 0.38 -2.7 5,278,653 + BT4051 0.52 +0.8 5,278,684 - BT4051 0.55 -2.7 5,278,696 - BT4051 0.57 +0.6 5,278,718 - BT4051 0.59 -1.9 5,278,731 + BT4051 0.60 -1.5 5,278,770 + BT4051 0.65 +0.3 5,278,774 + BT4051 0.65 +0.6 5,278,809 + BT4051 0.69 -2.7 5,278,836 + BT4051 0.72 -2.3 5,278,837 - BT4051 0.72 -2.7 5,278,840 + -1.4 5,278,960 + BT4051 0.85 +0.2 5,279,106 + +0.9 5,279,106 + -2.5 5,279,113 + -0.1 5,279,175 - -0.4 5,279,187 + +0.4 5,279,188 - +0.6 5,279,219 + +0.3 5,279,221 + +1.0 5,279,264 - -2.0 5,279,266 - +0.6 5,279,266 - -0.8 5,279,352 - -1.8 5,279,450 + BT4052 0.13 -1.3 5,279,450 + BT4052 0.13 -1.8 5,279,451 - BT4052 0.13 +1.6 5,279,451 - BT4052 0.13 -0.0 5,279,558 + BT4052 0.19 +0.6 5,279,559 - BT4052 0.19 +1.0 5,279,606 - BT4052 0.21 +0.6 5,279,697 - BT4052 0.26 -2.8 5,279,730 + BT4052 0.28 +1.6 5,279,752 - BT4052 0.29 -2.6 5,279,752 - BT4052 0.29 -0.5 5,279,760 - BT4052 0.30 -2.1 5,279,786 + BT4052 0.31 -1.3 5,280,086 + BT4052 0.47 +1.0
Or see this region's nucleotide sequence